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Tissue-specific extinguisher loci in the murine genome: A screening study based on a rat/mouse microcell hybrid panel

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Somatic Cell and Molecular Genetics

Abstract

Extinction of tissue-specific traits in intertypic somatic cell hybrids is a well-known phenomenon. In the past few years, microcell hybrids have been used in attempts to dissect this phenotype genetically, and tissue-specific extinguisher loci have been mapped to two different mouse chromosomes. When transferred from fibroblasts into hepatoma cells by microcell fusion, these loci down-regulate expression of specific liver genes intrans. However, other liver genes that are extinguished in genotypically complete hybrids seem not to be extinguished in monochromosomal hybrids. To assess the generality of monochromosomal extinction phenotypes, we assembled a collection of rat hepatoma/mouse fibroblast microcell hybrids that represent most of the mouse chromosome complement, and we screened them for expression of a large number of liver-specific genes. Phosphoenolpyruvate carboxykinase gene expression was down-regulated in hybrids containing mouse chromosome 7 or mouse chromosome 11, but other extinction phenotypes were not readily apparent. These results indicate that extinction of many liver genes may be a polygenic trait.

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Author notes

  1. Alan J. Schafer

    Present address: Department of Genetics, University of Cambridge, Downing Street, CB2 3EH, Cambridge, U.K.

  2. Gary A. Bulla

    Present address: Division of Cell and Developmental Biology, Pediatric Research Institute, 3662 Park Avenue, St., 63110, Louis, Missouri

Authors and Affiliations

  1. Department of Molecular Medicine, Fred Hutchinson Cancer Research Center, 1124 Columbia Street, 98104, Seattle, Washington

    Alan J. Schafer, Gary A. Bulla & R. E. K. Fournier

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Schafer, A.J., Bulla, G.A. & Fournier, R.E.K. Tissue-specific extinguisher loci in the murine genome: A screening study based on a rat/mouse microcell hybrid panel. Somat Cell Mol Genet 20, 195–213 (1994). https://doi.org/10.1007/BF02254760

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