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Insulin decreases the secretion of apoB-100 from hepatic HepG2 cells but does not decrease the secretion of apoB-48 from intestinal CaCo-2 cells

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Journal of Biomedical Science

Abstract

We compared the acute effect of insulin on the human colonic intestinal epithelial cell line CaCo-2 and the transformed human hepatic cell line HepG2. Over 24 h, 100 nM and 10 µM insulin significantly inhibited the secretion of apolipoprotein (apo) B-100 from HepG2 cells to 63 and 49% of control, respectively. Insulin had no effect on the secretion of apoB-48 from CaCo-2 cells. There was no effect of insulin on the cholesterol ester or free cholesterol concentrations in HepG2 or CaCo-2 cells. HepG2 and CaCo-2 cells bound insulin with high affinity, leading to similar stimulation of insulin receptor protein tyrosine kinase activation. Protein kinase C or mitogen-activated protein kinase activity in the presence or absence of insulin was not correlated with apoB-48 production in CaCo-2 cells. Therefore, insulin acutely decreases the secretion of apoB-100 in hepatic HepG2 cells, but does not acutely modulate the production or secretion of apoB-48 from CaCo-2 intestinal cells.

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Authors and Affiliations

  1. Laboratory for Cancer Medicine, University Department of Medicine, University of Western Australia, Royal Perth Hospital, Australia

    Andrew M. Thomson

  2. Western Australian Biomedical Research Institute, School of Biomedical Sciences, Curtin University of Technology, Perth, Australia

    Erik Helmerhorst

  3. Department of Nutrition, Dietetics and Food Sciences, Curtin University of Technology, GPO Box U1987, 6845, Perth, W.A., Australia

    Emma M. Allister, Sebely Pal &  John C. L. Mamo

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  1. Emma M. Allister
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Allister, E.M., Pal, S., Thomson, A.M. et al. Insulin decreases the secretion of apoB-100 from hepatic HepG2 cells but does not decrease the secretion of apoB-48 from intestinal CaCo-2 cells. J Biomed Sci 11, 789–798 (2004). https://doi.org/10.1007/BF02254364

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  • DOI: https://doi.org/10.1007/BF02254364

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