Psychopharmacology

, Volume 101, Issue 1, pp 27–33 | Cite as

Utility of an elevated plus-maze for the evaluation of memory in mice: effects of nootropics, scopolamine and electroconvulsive shock

  • Jiro Itoh
  • Toshitaka Nabeshima
  • Tsutomu Kameyama
Original Investigations

Abstract

An elevated plus-maze consisting of two open and two enclosed arms was employed for an evaluation of memory in mice. Mice in the plus-maze escaped from the open arm to the enclosed arm because mice apparently dislike open and high spaces. The time it took for the mice to move from the open arm to the enclosed arm (transfer latency) was recorded. The transfer latency after the 2nd day was significantly shorter than that on the 1st day when it was recorded at a rate of one trial a day for 5 days. The transfer latency on the 2nd day was significantly prolonged in the mice administered electroconvulsive shock (300 V, 1 s) or scopolamine (20 µg, ICV) immediately after the first trial compared to the transfer latency in the control group. The prolongation of transfer latency in the mice administered an electroconvulsive shock was reversed by pretreatment with aniracetam (20 mg/kg, PO), but not tacrine and physostigmine. The prolongation of transfer latency in the mice administered scopolamine was reversed by pretreatment with aniracetam (10 and 20 mg/kg, PO) tacrine (1 and 3 mg/kg, PO), or physostigmine (0.025–0.2 mg/kg, IP). These results suggest that transfer latency may be one of the parameters of learning and memory.

Key words

Memory Elevated plus-maze Aniracetam Tacrine (tetrahydroaminoacridine) Scopolamine 

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References

  1. Appleyard ME, Green AR, Greenfield SA (1987) Acetylcholinesterase activity rises in rat cerebrospinal fluid post-ictally; effect of a substantia nigra lesion on this rise and on seizure threshold. Br J Pharmacol 91:149–154PubMedGoogle Scholar
  2. Balfour DJK, Graham CA, Vale AL (1986) Studies on the possible role of brain 5-HT systems and adrenocortical activity in behavioural responses to nicotine and diazepam in an elevated X-maze. Psychopharmacology 90:528–532Google Scholar
  3. Bartus RT, Dean RL (1988) Tetrahydroaminoacridine, 3,4-diaminopyridine and physostigmine: direct comparison of effects on memory in aged primates. Neurobiol Aging 9:351–356PubMedGoogle Scholar
  4. Crutcher KA, Kesner RP, Novak JM (1983) Medial septal lesions, radial arm maze performance, and sympathetic sprouting: a study of recovery of function. Brain Res 262:91–98CrossRefPubMedGoogle Scholar
  5. Cumin R, Bandle EF, Gamzu E, Haefely WE (1982) Effects of the novel compound aniracetam (Ro 13-5057) upon impaired learning and memory in rodents. Psychopharmacology 78:104–111Google Scholar
  6. DeNoble VJ, Repetti SJ, Gelpke LW, Wood LM, Keim KL (1986) Vinpocetine: nootropic effects on scopolamine-induced and hypoxia-induced retrieval deficits of a step-through passive avoidance response in rats. Pharmacol Biochem Behav 24:1123–1128CrossRefPubMedGoogle Scholar
  7. Eclancher F, Ramirez JJ, Stein DG (1985) Neonatal brain damage and recovery: intraventricular injection of NGF at time of injury alters performance of active avoidance. Dev Brain Res 19:227–235CrossRefGoogle Scholar
  8. Ennaceur A, Delacour J (1988) A new one-trial test for neurobiological studies of memory in rats. 1: behavioral data. Behav Brain Res 31:47–59Google Scholar
  9. Flood JF, Landry DW, Jarvik ME (1981) Cholinergic receptor interactions and their effects on long-term memory processing. Brain Res 215:177–185Google Scholar
  10. Flood JF, Smith GE, Cherkin A (1988) Two-drug combinations of memory enhancers: effect of dose ratio upon potency and therapeutic window, in mice. Life Sci 42:2145–2154CrossRefPubMedGoogle Scholar
  11. Green AR, Metz A, Minchin MCW, Vincent ND (1987a) Inhibition of the rate of GABA synthesis in regions of rat brain following a convulsion. Br J Pharmacol 92:5–11PubMedGoogle Scholar
  12. Green AR, Heal DJ, Vincent ND (1987b) The effects of single and repeated electroconvulsive shock administration on the release of 5-hydroxytryptamine and noradrenaline from cortical slice of rat brain. Br J Pharmacol 92:25–30PubMedGoogle Scholar
  13. Haley TJ, McCormick WG (1957) Pharmacological effects produced by intracerebral injection of drugs in the conscious mouse. Br J Pharmacol 12:12–15Google Scholar
  14. Kameyama T, Nabeshima T, Kozawa T (1986) Step-down-type passive avoidance- and escape-learning method suitability for experimental amnesia models. J Pharmacol Methods 16:39–52CrossRefPubMedGoogle Scholar
  15. Krivanek J, McGaugh JL (1968) Effects of pentylenetetrazol on memory storage in mice. Psychopharmacologia 12:303–321CrossRefPubMedGoogle Scholar
  16. Linden D, Martinez Jr JL (1986) Leu-enkephalin impairs memory of an appetitive maze response in mice. Behav Neurosci 100:33–38CrossRefPubMedGoogle Scholar
  17. Lister RG (1987) The use of a plus-maze to measure anxiety in the mouse. Psychopharmacology 92:180–185PubMedGoogle Scholar
  18. LoConte G, Bartolini L, Casamenti F, Maroncini-Pepeu I, Pepeu G (1982) Lesions of cholinergic forebrain nuclei: changes in avoidance behavior and scopolamine actions. Pharmacol Biochem Behav 17:933–937CrossRefPubMedGoogle Scholar
  19. McGaugh JL, Alpern HP (1966) Effects of electroshock on memory: amnesia without convulsions. Science 152:665–666Google Scholar
  20. Miyamoto M, Shintani M, Nagaoka A, Nagawa Y (1985) Lesioning of the rat basal forebrain leads to memory impairments in passive and active avoidance tasks. Brain Res 328:97–104CrossRefPubMedGoogle Scholar
  21. Morris R (1984) Developments of a water-maze procedure for studying spatial learning in the rat. J Neurosci Methods 11:47–60CrossRefPubMedGoogle Scholar
  22. Moye TB, Vanderryn J (1988) Physostigmine accelerates the development of associative memory processes in the infant rat. Psychopharmacology 95:401–406CrossRefPubMedGoogle Scholar
  23. Nabeshima T, Yoshida S, Kameyama T (1988) Effects of the novel compound NIK-247 on impairment of passive avoidance response in mice. Eur J Pharmacol 154:263–269CrossRefPubMedGoogle Scholar
  24. Pellow S, File SE (1986) Anxiolytic and anxiogenic drug effects on exploratory activity in an elevated plus-maze: a novel test of anxiety in the rat. Pharmacol Biochem Behav 24:525–529CrossRefPubMedGoogle Scholar
  25. Pellow S, Chopin P, File SE, Briley M (1985) Validation of open: closed arm entries in an elevated plus-maze as a measure of anxiety in the rat. J Neurosci Methods 14:149–167CrossRefPubMedGoogle Scholar
  26. Perio A, Terranova JP, Worms P, Bluthe RM, Dantzer R, Biziere K (1989) Specific modulation of social memory in rats by cholinomimetic and nootropic drugs, by benzodiazepine inverse agonists, but not by psychostimulations. Psychopharmacology 97:262–268CrossRefPubMedGoogle Scholar
  27. Porsolt RD, Platel A (1982) Habituation of exploratory activity in mice a screening test for memory enhancing drugs. Psychopharmacology 78:346–352Google Scholar
  28. Porsolt RD, Lenègre A, Avril I, Stéru L, Doumont G (1987) The effects of exifone, a new agent for senile memory disorder, on two models of memory in the mouse. Pharmacol Biochem Behav 27:253–256CrossRefPubMedGoogle Scholar
  29. Quinton EE, Kramarcy NR (1977) Memory impairment correlates closely with cycloheximide dose and degree of inhibition of protein synthesis. Brain Res 131:184–190CrossRefPubMedGoogle Scholar
  30. Spangler EL, Rigby P, Ingram DK (1986) Scopolamine impairs learning performance of rats in a 14-unit T-maze. Pharmacol Biochem Behav 25:673–679Google Scholar
  31. Spignoli G, Pepeu G (1986) Oxiracetam prevents electroshock-induced decrease in brain acetylcholine and amnesia. Eur J Pharmacol 126:253–257CrossRefPubMedGoogle Scholar
  32. Spignoli G, Pepeu G (1987) Interactions between oxiracetam, aniracetam and scopolamine on behavior and brain acetylcholine. Pharmacol Biochem Behav 27:491–495Google Scholar
  33. Summers WK, Majovski LV, Marsh GM, Tachiki K, Kling A (1986) Oral tetrahydroaminoacridine in long-term treatment of senile dementia, Alzheimer type. New Engl J Med 315:1241–1245PubMedGoogle Scholar
  34. Wirsching BA, Beninger RJ, Jhamandas K, Boegman RJ, El-Defrawy SR (1984) Differential effects of scopolamine on working and reference memory of rats in the radial maze. Pharmacol Biochem Behav 20:659–662CrossRefPubMedGoogle Scholar
  35. Wolthuis OL (1981) Behavioural effects of etiracetam in rats. Pharmacol Biochem Behav 15:247–255CrossRefPubMedGoogle Scholar
  36. Yamada K, Inoue T, Tanaka M, Furukawa T (1985) Prolongation of latencies for passive avoidance response in rats treated with aniracetam or piracetam. Pharmacol Biochem Behav 22:645–648CrossRefPubMedGoogle Scholar

Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • Jiro Itoh
    • 3
  • Toshitaka Nabeshima
    • 1
    • 2
  • Tsutomu Kameyama
    • 1
  1. 1.Department of Chemical Pharmacology, Faculty of Pharmaceutical SciencesMeijo UniversityNagoyaJapan
  2. 2.Department of PharmacologyNippon Roche Research CenterKamakuraJapan
  3. 3.Dept. Hospital PharmacyNagoya University, School of MedicineNagoyaJapan

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