Journal of Biomedical Science

, Volume 3, Issue 4, pp 275–279 | Cite as

Endothelin-1 and insulin induce cellular inactivation of protein kinase FA/glycogen synthase kinase-3α in a common signaling pathway

  • Low-Tone Ho
  • You-Chung Chou
  • Jau-Song Yu
  • Shiaw-Der Yang
Original Paper


In this study, we investigate the effects of endothelin-1 (ET-1) and insulin on the cellular activity of protein kinase FA/glycogen synthase kinase-3α (kinase FA/GSK-3α) in rat adipocytes. The cellular activity of kinase FA/GSK-3α is inhibited to ∼ 50% of control within 30 min when cells are treated with 1 nM ET-1 at 37°C; in addition, significant inhibition to ∼ 60% of control is observed at as low as 1 pM ET-1. Conversely, ET-1 at concentrations up to 1 nM has no direct effect on purified kinase FA/GSK-3α in vitro. Immunoblotting analysis further reveals that the protein level of this kinase is not significantly changed when treated with 1 nM ET-1 for 30 min. Similar to ET-1, insulin as low as 10 nM can also induce inactivation of kinase FA/GSK-3α to ∼ 50% of control in adipocytes when processed under identical conditions. Most importantly, when treated with both insulin and ET-1, the activity of kinase FA/GSK-3α can be decreased only to ∼ 50% of control. Taken together, the results provide initial evidence that ET-1 and insulin may regulate this important multisubstrate/multifunctional protein kinase in a common signaling pathway in cells.

Key Words

Endothelin-1 Insulin Protein Kinase FA/GSK-3α MAP kinase signaling pathway Rat adipocytes 


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Copyright information

© National Science Council 1996

Authors and Affiliations

  • Low-Tone Ho
    • 1
  • You-Chung Chou
    • 2
  • Jau-Song Yu
    • 3
  • Shiaw-Der Yang
    • 4
  1. 1.Department of Medical Research and EducationVeterans General Hospital-TaipeiTaipei
  2. 2.Graduate Institute of Medical SciencesNational Defense Medical CenterTaipei
  3. 3.Department of Cell and Molecular Biology, Institute of Basic MedicineChang Gung Medical CollegeTao-Yuan
  4. 4.Institute of Biomedical SciencesNational Tsing Hua UniversityHsinchuTaiwan (ROC)

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