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Journal of Oceanography

, Volume 50, Issue 2, pp 165–171 | Cite as

Does the water retained in a filter paper affect the chlorophylla measurement?

  • Yuh-Ling Lee Chen
  • Shigemitsu Hara
Article
  • 45 Downloads

Abstract

The present study investigated the effects of water seeped in a filter paper on the variations of chlorophylla measurement. As little as 184 μl water retained in a 25 mm diameter GF/F filter paper after filtration resulted in the settlement of 60% chlorophylla at the bottom 20% of the extractant (90% acetone) in the extraction tube. More than one order difference in concentration of chlorophylla was found between the uppermost 20% and the bottom 20% of the extractant. Chlorophylla is trapped at the bottom by a pycnocline created by the water seeped into the filter paper and by chlorophylla itself, which is heavier than the extractant. Once the extractant was well mixed, good reproducibility of measurement was obtained. White precipitation occurred during the mixing of seawater sample and the extractant and was not found to affect the measurement of chlorophylla. The dilution of the extractant by the seawater seeped in the filter paper during extraction probably did not affect the extraction efficiency.

Keywords

Precipitation Filtration Acetone Filter Paper Extraction Efficiency 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Jeffrey, S. W. and G. M. Hallegraeff (1987): Chlorophyllase distribution in ten classes of phytoplankton: a problem for chlorophyll analysis.Mar. Ecol. Prog. Ser.,35, 293–304.Google Scholar
  2. JGOFS (1989): Joint Global Ocean Flux Study Core Measurement Protocols, JGOFS REPORT No. 6. Scientific Committee on Oceanic Research, 40 pp.Google Scholar
  3. Lean, D. R. S. and B. K. Burnison (1979): An evaluation of errors in the14C method of primary production measurement.Limnol. Oceanogr.,24, 917–928.Google Scholar
  4. Li, W. K. W. (1990): Particles in “particle-free” seawater: Growth of ultraphytoplankton and implications for dilution experiments.Can. J. Fish. Aquat. Sci.,47, 1258–1268.Google Scholar
  5. Munawar, M., I. F. Munawar, P. E. Ross and A. Dagenais (1982): Microscopic evidence of phytoplankton passing through glass-fiber filters and its implication for chlorophyll analysis.Arch. Hydrobiol.,94, 520–528.Google Scholar
  6. Phinney, D. A. and C. S. Yentsch (1985): A novel phytoplankton chlorophyll technique: toward automated analysis.J. Plankton Res.,7, 633–642.Google Scholar
  7. Stockner, J. G., M. E. Klut and W. P. Cochlan (1990): Leaky filters: a warning to aquatic ecologist.Can. J. Fish. Aquat. Sci.,47, 16–23.Google Scholar
  8. Strickland, J. D. H. and T. R. Parsons (1972):A Practical Handbook of Seawater Analysis. 2nd ed. Bull. Fish. Res. Bd. Can., 167, 310 pp.Google Scholar
  9. Suzuki, R. and Y. Fujita (1986): Chlorophyll decomposition inSkeletonema costatum: a problem in chlorophyll determination of water samples.Mar. Ecol. Prog. Ser.,28, 81–85.Google Scholar
  10. Suzuki, R. and T. Ishimaru (1990): An improved method for the determination of phytoplankton chlorophyll using N, N-dimethylformamide.J. Oceanogr. Soc. Japan,46, 190–194.CrossRefGoogle Scholar
  11. Taguchi, S. and E. A. Laws (1988): On the microparticles which pass through glass fiber filter type GF/F in coastal and open waters.J. Plankton Res.,10, 999–1008.Google Scholar
  12. Venrick, E. L. (1987): On fluorometric determinations of filter-retained pigments.Limnol. Oceanogr.,32, 492–493.Google Scholar
  13. Venrick, E. L. and T. L. Hayward (1984): Determining chlorophyll on the 1984 CalCOFI surveys.CalCOFI Report,25, 74–79.Google Scholar
  14. Yentsch, C. S. and D. W. Menzel (1963): A method for the determination of phytoplankton chlorophyll and phaeophytin by fluorescence.Deep-Sea Res.,10, 221–231.Google Scholar

Copyright information

© Journal of the Oceanographic Society of Japan 1994

Authors and Affiliations

  • Yuh-Ling Lee Chen
    • 1
  • Shigemitsu Hara
    • 1
  1. 1.Department of Marine ResourcesNational Sun Yat-sen UniversityKaohsiungTaiwan, ROC

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