Journal of Biomedical Science

, Volume 5, Issue 3, pp 185–191 | Cite as

Bcl-2 blocks apoptotic signal of transforming growth factor-β in human hepatoma cells

  • Yu-Lun Huang
  • Chen-Kung Chou
Original Paper


Transforming growth factor-β (TGF-β) has been shown to induce apoptosis on normal hepatocytes and hepatoma cells both in vitro and in vivo. However, how the TGF-β induces apoptosis is still not clear. We examined the expression of anti-apoptosis proteins and sensitivity to TGF-β in three well differentiated human hepatoma cell lines. Two TGF-β sensitive cell lines Hep3B and HuH7 totally lacked Bcl-2. In contrast, the TGF-β resistant HepG2 cells expressed a substantial amount of Bcl-2. All three cell lines expressed equal amounts of Bcl-XL, Bcl-XS and Bax. Overexpression of Bcl-2 in Hep3B and HuH7 cells protected them from TGF-β-induced apoptosis. TGF-β treatment increased intracellular peroxide production and suppressed the expression of glutathione-S-transferase in the Hep3B cells, and these effects were partially suppressed by the overexpression of Bcl-2. These results suggest that Bcl-2 may protect cell from TGF-β-F-induced apoptosis by interfering TGF-β generated signals leading to induce reactive oxygen species production.

Key Words

TGF-β Bcl-2 Apoptosis Antioxidative enzyme Reactive oxygen species 


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Copyright information

© National Science Council 1998

Authors and Affiliations

  • Yu-Lun Huang
    • 1
  • Chen-Kung Chou
    • 1
    • 2
    • 3
  1. 1.Institute of BiochemistryNational Yang-Ming UniversityTaiwan
  2. 2.Department of Medical ResearchVeterans General HospitalTaipeiTaiwan
  3. 3.Division of Molecular and Genomic MedicineNational Health Research InstitutesTaipeiTaiwan

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