Journal of Biomedical Science

, Volume 7, Issue 6, pp 459–465 | Cite as

Apoptosis occurs in a new model of thermal brain injury

  • Jeff W. Chen
  • Julian Lin
  • N. Madamanchi
  • Todd T. Trier
  • Gerald Campbell
Original Paper


Apoptosis has been implicated recently as a prominent response of the brain to a variety of insults, such as ischemia and trauma. In this study, we demonstrate that apoptosis is a prominent part of the brain's response to a thermal insult. To examine the brain's response to a thermal insult, a new model of thermal brain injury in the laboratory rat was developed. Water heated to 60°C was passed over an area of thinned calvarium for 1 min. This resulted in an actual brain temperature of 47–48°C. A uniform area of 2,3,5-triphenyl-tetrazolium chloride pallor was demonstrated and pyknotic neurons were seen in the area of injury by hematoxylin-eosin staining. Apoptosis was demonstrated by the characteristic DNA fragmentation seen by agarose gel electrophoresis, ApopTag in situ staining and electron microscopy. The findings of apoptosis were localized to the area of thermal injury and were time dependent, starting 6 h after the insult and peaking approximately 18 h after the insult. This represents one of the first demonstrations that apoptosis occurs in the brain in response to a thermal injury.

Key Words

Apoptosis Thermal brain injury 


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Copyright information

© National Science Council 2000

Authors and Affiliations

  • Jeff W. Chen
    • 1
    • 3
  • Julian Lin
    • 1
  • N. Madamanchi
    • 1
  • Todd T. Trier
    • 1
  • Gerald Campbell
    • 2
  1. 1.Division of Neurosurgery, Department of SurgeryUniversity of Texas Medical Branch at GalvestonGalvestonUSA
  2. 2.Division of Neuropathology, Department of PathologyUniversity of Texas Medical Branch at GalvestonGalvestonUSA
  3. 3.Division of Neurotrauma, Department of SurgeryLegacy Emanuel HospitalPortlandUSA

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