AP1-Related factors interact with simian immunodeficiency virus long terminal repeats
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The SF1 element (nucleotide −135 to −131) in the U3 region of the simian immunodeficiency virus (SIV) 5′ to the NF-κB binding site plays a role in basal expression of SIV [Winandy et al., J Virol 66:5216–5223, 1992]. Using the electrophoretic mobility shift assay, we have characterized a specific SF1 binding factor (sf1) in the nuclear fractions of transformed T cells as well as monocytic and macrophage leukemic cells. A strong SF1 binding activity was demonstrated in all cell types. The SF1 binding activity was unaffected by treatment with PMA, PHA, or Ca2+ ionophore. The HIV-1 long terminal repeat (LTR) 5′ to the NF-κB site contained a homologous element which showed a specific binding complex of different mobility. The sf1 interacting with SIV LTR was composed of multiple proteins judged from UV crosslinking, some of which might be related to Jun/Fos proteins.
Key WordsAIDS Retrovirus Transcription factor Affinity chromatography Gel mobility shift assay UV crosslinking
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