Determination of bradykinin-(1–5) in inflammatory exudate by a new ELISA as a reliable indicator of bradykinin generation
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We have developed an ELISA for BK-(1–5) (Arg1-Pro2-Pro3-Gly4-Phe5). In rat carrageenin-induced pleurisy, in which a plasma exudation peak was observed 5 h after carrageenin, BK levels in the exudates were negligible (<60 pg/rat). BK-(1–7) (des-Phe8-Arg9-BK) was detectable (900–400 pg/rat) over the entire course of the inflammation. However, a larger amount of BK-(1–5) was detectable in association with the increase in plasma exudation, showing a peak (8800±1200 pg/rat) 3 h after carrageenin. Bromelain (10 mg/kg, i.v.) and soy bean trypsin inhibitor (0.3 mg/rat, intra-pleural) significantly reduced BK-(1–5) levels (by 60–93%, 3, 7 and 19 h after carrageenin) and plasma exudation rates (by 61–74%, 3 and 7 h after carrageenin). Dexamethasone (0.3 mg/kg, i.p.) reduced BK-(1–5) levels (by 78%) and decreased plasma exudation (by 70%) 3 h after carrageenin. In nasal allergy patients, antigen challenge of nasal mucosa elevated BK-(1–5) levels and active kallikrein levels in nasal washes. These results verify that BK-(1–5) determined by ELISA is a good indicator for release of kinins in vivo.
Key wordsEnzyme-linked immunosorbent assay (ELISA) Bradykinin-(1–5) Carageenin-induced pleurisy Nasal allergy Kinin
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- Majima M, Ueno A, Sunahara N, Katori M. Measurement of des-Phe8-Arg9-bradykinin by enzyme immunoassay: A useful parameter of plasma kinin release. In: Abe K, Moriya H, Fujii S, editors Kinin V, Part B. Adv Exp Med Biol Vol 247B. New York: Plenum, 1989:535–40.Google Scholar
- Ferreira SH, Vane JR. The disappearance of bradykinin and eledoisin in the circulation and vascular beds of the cat. Br J Pharmacol 1967;30:417–24.Google Scholar
- Bonner G, Iwerson D, Shimamoto K. The analytical value for kinin concentration in blood depends on the antiserum used in the bradykinin radioimmunoassay. J Clin Chem Biochem 1987;25:39–43.Google Scholar
- Katori M, Majima M, Odoi-Adome R, Sunahara N, Uchida Y. Evidence for the involvement of a plasma kallikrein-kinin system in the immediate hypotension produced by endotoxin in anaesthetized rats. Br J Pharmacol 1989;78:1383–91.Google Scholar
- Naclerio RM, Meier HL, Kagey-Sobotka A, Adkinson NF, Meyers DA, Norman PS, et al. Mediator release after nasal airway challenge with allergen. Am Rev Resp Dis 1983;128:597–602.Google Scholar
- Okuda M. Nasal provocation testing. Am J Rhinol 1989;3:83–92.Google Scholar
- Katori M, Harada Y, Ueno A, Majima M, Oda T, Kawamura M. Modulation of plasma exudation by PGE2 and that of leukocyte migration by LTB4 in inflammatory models. Dermatologia 1989;179:60–3.Google Scholar
- Katori M, Majima M, Harada Y, Ueno A. A significant role of plasma kallikrein-kinin system in plasma exudation of rat carrageenin-induced pleurisy. In: Abe K, Moriya H, Fujii S, editors. Adv Exp Med Biol Vol 247A. New York: Plenum, 1989:137–44.Google Scholar