Effect of yeast extract, peptone, and certain nitrogen compounds on sporulation ofSaccharomyces cerevisiae
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Aeration of cells for 24 hrs. previous to placing them in 0.1% sodium acetate solution diminished sporulation, but this decrease was overcome by the addition of 0.1% yeast extract to the acetate solution. Cells starved by growth on Czapek solution agar +0.03% peptone formed very few ascospores in acetate solution. The addition of yeast extract or peptone in low content to the acetate solution increased the yields. However, the cells did not form as many ascospores as well-nourished cells in acetate solution.
A comparison was made of the sporulation of cells from basal presporulation medium containing, separately, 18 nitrogen sources. In general, nitrogen sources that supported growth gave cells that sporulated well. Tyrosine and tryptophan were exceptions.
Cells multiplied in basal medium with the nitrogen source deleted formed no asci in 0.1% acetate solution. When nitrogen sources were added to the acetate solution, many stimulated sporulation. Yields of asci in these sporulation cultures were, however, lower than the yield obtained from well-nourished cells in 0.1% acetate solution.
KeywordsAgar Tyrosine Tryptophan Yeast Extract Aeration
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