A brief method for analyzing Rotorod® samples for pollen content
- 153 Downloads
Analyzing Rotorod® pollen samples can be time consuming when one uses the standard method of evaluating an entire collector rod. This present investigation explored an abbreviated analysis method which is indicated by Poisson statistical theory. The authors systematically analyzed 18 Rotorod pollen samples from Spring 1994 which contained 408–7394 pollen grains per rod. The atmospheric pollen concentration (pollen grains/m3) was calculated from the number of pollen grains contained on the entire rod surface (Ptotal) and a sub-area containing at least 400 grains (P>400). The estimate of the atmospheric pollen concentration resulting from Ptotal and P>400 for each rod did not vary by more than ±9% (mean, 2.0%±3.7). These data indicate that pollen grains populated the sample rods rather uniformly, suggesting a mode of random recovery from the atmosphere. This study's results are consistent with the expectations concerning a Poisson process and support analyzing collector rods until a threshold number of pollen grains is counted.
KeywordsRotorod Sampler Pollen grains Poisson statistics Aerobiology
Unable to display preview. Download preview PDF.
- Brown, T., Frenz, D.A., Wimpsett, T.L., Barr, D.W. and Hokanson, J.M. (1993) Operating Instructions for the Rotorod Sampler. Sampling Technologies, Inc., Minnetonka.Google Scholar
- Chapman, J.A. (1982) The enhancement of the practice of clinical allergy with daily pollen and spore counts. Immunol. Allergy Pract. 4, 13–18.Google Scholar
- Gay, L.N., Curtis, H., and Norris, T. (1941) A pollen survey of the island of Bermuda. Bull. Johns Hopkins Hosp. 68, 179–189.Google Scholar
- Girshick, M.A., Rubin, H. and Sitgreaves, R. (1955) Estimates of bounded relative error in particle counting. Ann. Math. Stat. 26, 276–285.Google Scholar
- Grinnell, S.W., Perkins, W.A. and Vaughan, L.M. (1961) Sampling Apparatus and Method. Patent no. 2,973,642. United States Patent Office, Washington.Google Scholar
- Noll, K.E. (1970) A rotary inertial impactor for sampling giant particles in the atmosphere. Atmos. Environ. 4, 9–19.Google Scholar
- Segale, J.D. (1994) The AAAI Aeroallergen Network 1994 Information Sheet. American Academy of Allergy and Immunology, Milwaukee.Google Scholar
- Solomon, W.R., Burge, H.A., Boise, J.R. and Becker, M. (1980) Comparative particle recoveries by the retracting Rotorod, Rotoslide and Burkard Spore Trap sampling in a compact array. Int. J. Biometeorol. 24, 107–116.Google Scholar