Psychopharmacology

, Volume 110, Issue 1–2, pp 245–253 | Cite as

Attenuation of antipredator defensive behavior in rats following chronic treatment with imipramine

  • Robert J. Blanchard
  • Jon K. Shepherd
  • R. John Rodgers
  • Linda Magee
  • D. Caroline Blanchard
Original Investigations

Abstract

The anxiety/defense test battery has been developed to assess defensive reactions in rats to situations associated with a natural predator, the domestic cat. This comprises three paradigms designed to study the effects of cat exposure on general activity and location with respect to the cat (proxemic avoidance), the effects of cat exposure on non-defensive consummatory behavior, and the behavioral response to cat odor. In the present study subjects were exposed to 21 days pretreatment with imipramine (0, 5, and 15 mg/kg), before being assessed in the three experimental paradigms (carried out over a total period of 7 days). Imipramine treatment was maintained on a daily basis during the 7 days taken to complete the series of tests. The data indicated a behaviorally specific profile consistent with anxiety/fear reduction, but not with sedation, in three different paradigms, following treatment with 15 mg/kg imipramine. These behavioral changes included a reduction in freezing, proxemic avoidance and a disinhibition of suppressed feeding in response to cat presentation. Similarly, imipramine treatment (15 mg/kg) significantly reduced behaviors associated with risk assessment (e.g. flat back approach, stretch attend) during presentation of a cat odor stimulus. This behavioral profile suggests that chronic pretreatment with imipramine produces an attenuation of antipredator defensive behavior.

Key words

Imipramine Antipanic Tricyclic antidepressant Chronic treatment Anxiety Defense Fear Risk assessment Animal model Sex differences 

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References

  1. Ballenger JC (1990) Neurobiology of panic disorder. Wiley-Liss, New YorkGoogle Scholar
  2. Bernstein JG (1987) Induction of obesity by psychotropic drugs. In: Wurtman RJ, Wurtman JJ (eds) Human obesity. Ann NY Acad Sci 499:203–215Google Scholar
  3. Blanchard RJ, Blanchard DC (1989) Antipredator defensive behaviors in a visible burrow system. J Comp Psychol 103:70–82Google Scholar
  4. Blanchard RJ, Blanchard DC, Hori K (1989) Ethoexperimental approaches to the study of defensive behavior. In: Blanchard RJ, Brain PF, Blanchard DC (eds) Ethoexperimental approaches to the study of behavior. Martinus Nijhoff, Dordecht, pp 114–136Google Scholar
  5. Blanchard DC, Blanchard RJ, Tom P, Rodgers RJ (1990a) Diazepam changes risk assessment in an anxiety/defense test battery. Psychopharmacology 101:511–518Google Scholar
  6. Blanchard RJ, Blanchard DC, Weiss S (1990b) Ethanol effects in an anxiety/defense test battery. Alcohol 7:375–381Google Scholar
  7. Blanchard RJ, Blanchard DC, Weiss SM, Mayer S (1990c) Effects of ethanol and diazepam on reactivity to predatory odors. Pharmacol Biochem Behav 35:775–780Google Scholar
  8. Blanchard DC, Shepherd JK, Rodgers RJ, Blanchard RJ (1992) Evidence for differential effects of 8-OH-DPAT on male and female rats in the anxiety/defense test battery. Psychopharmacology (in press)Google Scholar
  9. Blanchard DC, Young B, Blanchard RJ, Rodgers RJ (1991b) Gepirone effects on defensive behaviors in an anxiety/defense test battery. Pharmacol Biochem Behav (in press)Google Scholar
  10. Bodnoff SR, Suranyi-Cadotte B, Aitken DH, Quirion R, Meaney MJ (1988) The effects of chronic antidepressant treatment in an animal model of anxiety. Psychopharmacology 95:298–302Google Scholar
  11. Bodnoff SR, Suranyi-Cadotte B, Quirion R, Meaney MJ (1989) A comparison of the effects of diazepam versus several typical and atypical antidepressant drugs in an animal model of anxiety. Psychopharmacology 97:277–279Google Scholar
  12. Cassella JV, Davis M (1985) Fear-enhanced acoustic startle is not attenuated by acute or chronic imipramine treatment in rats. Psychopharmacology 87:278–282Google Scholar
  13. Chouinard G, Annable L, Fontaine R, Solyom L (1982) Alprazolam in the treatment of generalized anxiety and panic disorders: a double-blind, placebo-controlled study. Psychopharmacology 77:229–233Google Scholar
  14. Egan J, Earley CJ, Leanord BE (1979) The effects amitriptyline and mianserin (ORG GB94) on food-motivated behavior of rats trained in a runway: possible correlations with biogenic amine concentration in the limbic system. Psychopharmacology 61:143–147Google Scholar
  15. Fernstrom MH (1989) Depression, antidepressants and body weight change. In: Schneider LH, Cooper SJ, Halmi KA (eds) The psychobiology of human eating disorders. Ann NY Acad Sci 575:31–40Google Scholar
  16. File SE (1990) Preclinical studies of the mechanisms of anxiety and its treatment. In: Ballenger (ed) Neurobiology of panic disorder. Wiley-Liss, New York, pp 31–50Google Scholar
  17. File SE, Johnston AL (1987) Chronic treatment with imipramine does not reverse the effects of 3 anxiogenic compounds in a test of anxiety in the rat. Neuropsychobiology 17:187–192Google Scholar
  18. Fontana DJ, Commissaris RL (1988) Effects of acute and chronic imipramine administration on conflict behavior in the rat: a potential “animal model” of panic disorder? Psychopharmacology 95:147–150Google Scholar
  19. Fontana DJ, Carbary TJ, Commissaris RL (1989) Effects of acute and chronic anti-panic drug administration on conflict behavior in the rat. Psychopharmacology 98:157–162Google Scholar
  20. Gloger S, Grunhaus L, Bimacher B, Troudart T (1981) Treatment of spontaneous panic attacks with clomipramine. Am J Psychiatry 138:1215–1217Google Scholar
  21. Hendrie CA, Neill JC (1991) An animal model of panic disorder. Paper presented at the Annual Meeting of the British Association for Psychopharmacology, York, July 21–24Google Scholar
  22. Johnston AL (1990) The implication of noradrenaline in anxiety. In: Briley P, File, SE (eds) New concepts in anxiety. Macmillan, London, pp 347–365Google Scholar
  23. Kilts CD, Commissaris RL, Rech RH (1981) Comparison of anticonflict drug effects in three experimental models of anxiety. Psychopharmacology 74:290–296Google Scholar
  24. Lader M, Petursson H (1983) Long-term effects of benzodiazepines. Neuropharmacology 22:527–533Google Scholar
  25. Nobrega JN, Coscina DV (1982) Effects of chronic antidepressant treatment on feeding behavior in rats. In: Hoebel BG, Novin D (eds) The neural basis of feeding and reward. Haer Institute, pp 525–534Google Scholar
  26. Nutt DJ (1990) Anxiety and its therapy: today and tomorrow. In: Briley P, File SE (eds) New concepts in anxiety. Macmillan, London, pp 1–13Google Scholar
  27. Ookuma K, Sakata T, Fujimoto K (1990) Evidence for feeding elicited through antihistaminergic effects of tricyclic antidepressants in the rat hypothalamus. Psychopharmacology 101:481–485Google Scholar
  28. Owen RT, Tyrer P (1983) Benzodiazepine dependence: a review of the evidence. Drugs 25:385–398Google Scholar
  29. Pellow S, File SE (1987) Can anti-panic drugs antagonize the anxiety produced in the rat by drugs acting at the GABA-benzodiazepine receptor complex? Neuropsychobiology 17:60–65Google Scholar
  30. Poschel BPH (1971) A simple and specific screen for benzodiazepine like drugs. Psychopharmacologia 19:1193–1203Google Scholar
  31. Rickels K and Schweizer E (1987) Current pharmacotherapy of anxiety and panic. In: Meltzer HY (ed) Psychopharmacology the third generation of progress. Raven Press, New York, pp 1193–1203Google Scholar
  32. Sheehan DV (1986) The tricyclic antidepressants in the treatment of panic and anxiety disorders. Psychomimetics 27:10–16Google Scholar
  33. Sheehan DV, Ballenger J, Jacobsen G (1980) Treatment of endogenous anxiety with phobic, hysterical and hypochondrial symptoms. Arch Gen Psychiatry 37:51–59Google Scholar
  34. Shepherd JK, Flores T, Rodgers RJ, Blanchard RJ, Blanchard DC (1991) The anxiety/defense test battery: influence of gender and ritanserin treatment on antipredator defensive behavior. Physiol Behav (submitted)Google Scholar
  35. Tyrer P (1986) Drug treatment in psychiatry. In: Hill P, Murray R, Thorley A (eds) Essentials of postgraduate psychiatry, 2nd edn. Grunne and Stratton, London, pp 641–666Google Scholar
  36. Von Voightlander PF, Straw RN (1985) Alprazolam: review of pharmacological, pharmacokinetic and clinical data. Drug Dev Res 6:1–12Google Scholar
  37. Westenberg HGM, den Boer JA, Kahn RS (1987) Psychopharmacology of anxiety disorders: on the role of serotonin in the treatment of anxiety states and phobic disorders. Psychopharmacol Bull 23:145–149Google Scholar
  38. Willner P, Montgomery T (1980) Attenuation of amphetamine anorexia in rats following subchronic treatment with a tricyclic antidepressant. Commun Psychopharmacol 4:101–106Google Scholar

Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • Robert J. Blanchard
    • 1
    • 3
  • Jon K. Shepherd
    • 1
  • R. John Rodgers
    • 4
  • Linda Magee
    • 1
  • D. Caroline Blanchard
    • 1
    • 2
  1. 1.Bekesy Laboratory of NeurobiologyUSA
  2. 2.Department of Anatomy and Reproductive BiologyJohn A. Burns School of MedicineUSA
  3. 3.Department of PsychologyUniversity of HawaiiHonoluluUSA
  4. 4.Department of PsychologyUniversity of LeedsLeedsUK

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