Vesamicol, an acetylcholine uptake blocker in presynaptic vesicles, suppresses rapid eye movement (REM) sleep in the rat
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Vesamicol inhibits acetylcholine uptake in presynaptic vesicles and reduces its release. The present study was performed in order to test the effects of this drug in a cholinergic related function as rapid eye movement (REM) sleep. Wistar male rats were implanted for sleep recordings. In addition, a stainless steel cannula was implanted into the left lateral ventricle for intracerebroventricular (ICV) injections. In experiment 1, a dose-response curve was performed. Saline or vesamicol (20, 40, 80 and 100 µg) were injected. Following the ICV injections, animals' sleep was recorded for 8 h. In experiment 2, after adaptation and baseline recordings, animals received 50 µg vesamicol ICV at 1000 hours. every 24 h for 2 consecutive days. After each injection an 8-h sleep recording session was performed. Two subsequent recovery recordings were allowed. Results obtained in experiment 1 showed a dose-response reduction of REM sleep with significant values at 80 µg and 100 µg of vesamicol. The main findings in experiment 2 were a reduction in REM sleep time and an increases in REM sleep latency. On the recovery days, a dramatic rebound of REM sleep was observed. Vesamicol behaved as an anticholinergic drug. It produced a reduction in REM sleep time and a rebound of this sleep stage after its withdrawal.
Key wordsVesamicol Acetylcholine REM sleep Sleep Rats
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- Gillin JC, Salín-Pascual RJ, Velazquez-Moctezuma J, Shiromani P, Zoltoski R (1993) Cholinergic receptor subtypes and REM sleep in animals and normal controls. In: Cuello AC (ed) Progress in brain research 98. Elsevier, Amsterdam, pp 379–387Google Scholar
- Ursin R, Sterman MB (1968) A manual for standard scoring of sleep waking states in the adult cat. UCLA, Public Services Dept, Los Angeles, Calif.Google Scholar
- Velázquez-Moctezuma J, Gillin JC, Shiromani PJ (1989) Effect of Specific M1, M2 muscarinic receptor agonist on REM sleep generation. Brain Res 503:128–131Google Scholar