Abstract
The present study examined the effects of electroconvulsive shock (ECS) on interstitial concentrations of dopamine (DA), its metabolites DOPAC and HVA and the serotonin metabolite 5-HIAA in the striatum of freely moving rats using on-line microdialysis. DA increased sharply following a single ECS. Interstitial concentrations of DOPAC, HVA and 5-HIAA also increased significantly. The ECS-induced increase in DA varied as a function of days following implantation of the microdialysis probe, being 1300%, 305% and 300% of baseline 24, 48 and 72 h after surgery, respectively. In contrast, the response of the metabolites to ECS did not differ across days following surgery, being approximately 130%, 140% and 110% of baseline for DOPAC, HVA and 5-HIAA, respectively. Seizure activity induced by the convulsant agent flurothyl did not influence dialysate DA concentrations, suggesting that the ECS-induced DA release was related to the passage of current and not to the seizure activity. Interstitial concentrations of acetylcholine and choline in the striatum increased by approximately 20% and 140%, respectively, in response to a single ECS. The DA (but not the DOPAC or HVA) response to ECS was refractory to a second ECS delivered 2 h after the first. A second ECS delivered 24 h after the first produced the normal increase in DA. The ECS-induced increase in DA was attenuated following repeated ECS (eight treatments, one every second day). Baseline DOPAC and HVA concentrations were significantly elevated by repeated ECS. These results demonstrate that acute ECS produces a transient and somewhat selective increase in extracellular concentrations of DA, that this effect is reduced after repeated ECS, and that the increase is not due to the seizure activity itself. They also indicate that repeated ECS produces presynaptic change compatible with increase DA synthesis and/or turnover. These findings may be relevant to recent reports of ECS efficacy in the treatment of Parkinson's disease.
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Abbreviations
- DA :
-
dopamine
- DOPAC :
-
3,4-dihydroxyphenylacetic acid
- HVA :
-
homovanillic acid
- 5-HIAA :
-
5-hydroxyindoleacetic acid
- ACh :
-
acetylcholine
- ECS :
-
electroconvulsive shock
- ECT :
-
electroconvulsive therapy
References
Abrams R (1988) Electroconvulsive therapy. Oxford University Press, New York
Abrams R (1989) ECT for Parkinson's disease. Am J Psychiatry 46:1391–1393
Anderson K, Balldin J, Gottfried CG (1987) A double-blind evaluation of electroconvulsive therapy in Parkinson's disease with “on-off” phenomena. Acta Neurol Scand 76:191–199
Atterwill CK (1984) The effect of ECS on central cholinergic and interrelated neurotransmitter systems. In: Lerer B, Weiner RD, Belmaker RD (eds) ECT: basic mechanisms. Libbey, London pp 79–88
Benveniste H (1989) Brain microdialysis. J Neurochem 52:1667–1679
Chiodo LA, Antelman SM (1980) Electroconvulsive shock: progressive dopamine autoreceptor subsensitivity independent of repeated treatment. Science 210:799–801
Damsma G, Day J, Fibiger HC (1989) Lack of tolerance to nicotine-induced dopamine release in the nucleus accumbens. Eur J Pharmacol 168:363–368
Damsma G, Westerink BHC, de Vries JB, van den Berg CJ, Horn AS (1987) Measurement of acetylcholine release in freely moving rats by means of automated intracerebral dialysis. J Neurochem 48:1523–1528
Douyon R, Serby M, Klutchko B, Rotrosen J (1989) ECT and Parkinson's disease revisited: a “naturalistic” study. Am J Psychiatry 146:1451–1455
Fibiger HC (1990) The dopamine hypothesis of schizophrenia and mood disorders: contradictions and speculations. In: Willner P, Scheel-Kruger J (eds) The mesolimbic dopamine system: from motivation to action. Wiley, Chichester, England in press
Fink M (1979) Convulsive therapy: theory and practice. Raven Press, New York
Fochtmann LJ, Cruciani R, Aiso M, Potter WZ (1989) Chronic electroconvulsive shock increases D-1 receptor binding in rat substantia nigra. Eur J Pharmacol 167:305–306
Glue P, Costello MJ, Pert A, Mele A, Nutt DJ (1990) Regional neurotransmission responses after acute and chronic electronconvulsive shock. Psychopharmacology 100:60–65
Grahame-Smith DG (1984) The neuropharmacological effects of electroconvulsive shock and their relationship to the therapeutic effect of electroconvulsive therapy in depression. Adv Biochem Psychopharmacol 39:327–343
Green AR, Nutt DJ (1987) Psychopharmacology of repeated seizures: Possible relevance to the mechanism of action of electroconvulsive therapy. In: Iversen LL, Iversen SD, Snyder SH (eds) Handbook of Psychopharmacology. Vol. 19, Plenum Press, New York, pp 375–419
Imperato A, Di Chiara G (1984) Trans-striatal dialysis coupled to reverse phase high performance liquid chromatography with electrochemical detection: a new method for the study of the in vivo release of endogenous dopamine and metabolites. J Neurosci 4:966–977
Lerer B (1987) Neurochemical and other neurobiological consequences of ECT: implications for the pathogenesis and treatment of affective disorders. In: Meltzer HY (ed) Psychopharmacology: the third generation of progress. Raven Press, New York, pp 577–587
Modigh K, Balldin J, Eriksson E, Granerus AK, Walinder J (1984) Increased responsiveness of dopamine receptor after ECT: A review of experimental and clinical evidence. In: Lerer B, Weiner RD, Belmaker RH (eds) ECT: basic mechanisms. Libbey, London, pp 18–27
Nomikos GG, Zis AP, Damsma G, Fibiger HC (1990) Electroconvulsive shock produces large increases in interstitial concentrations of dopamine in the rat striatum an in vivo microdialysis study. Neuropsychopharmacology (in press)
Paxinos G, Watson C (1986) The rat brain in stereotaxic coordinates. Academic Press, London
Reiziz J, Mena MA, Bazam E, Muradas V, Lerma J, Delgado JMR, De Yebenes JG (1989) Temporal profile of levels of monoamines and their metabolites in striata of rats implanted with dialysis tubes. J Neurochem 53:789–792
Serra G, Argiolas A, Fadda F, Melis MR, Gessa GL (1981) Repeated electroconvulsive shock prevents the sedative effect of small doses of apomorphine. Psychopharmacology 73:194–196
Westerink BHC, Tuinte MHJ (1986) Chronic use of intracerebral dialysis for the in vivo measurement of 3,4-dihydroxyphenylethylamine and its metabolite 3,4-dihydroxyphenylacetic acid. J Neurochem 46:181–185
Westerink BHC, de Vries JB (1988) Characterization of in vivo dopamine release as determined by brain microdialysis after acute and subchronic implantations: methodological aspects. J Neurochem 51:683–687
Westerink BHC, Damsma G, Rollema H, de Vries JB, Horn AD (1987) Scope and limitations of in vivo brain dialysis: a comparison to its applications to various neurotransmitter systems. Life Sci 41:1763–1776
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Zis, A.P., Nomikos, G.G., Damsma, G. et al. In vivo neurochemical effects of electroconvulsive shock studied by microdialysis in the rat striatum. Psychopharmacology 103, 343–350 (1991). https://doi.org/10.1007/BF02244288
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DOI: https://doi.org/10.1007/BF02244288