, Volume 100, Issue 4, pp 558–559 | Cite as

Antianxiety effect of cannabidiol in the elevated plus-maze

  • F. S. Guimarães
  • T. M. Chiaretti
  • F. G. Graeff
  • A. W. Zuardi
Rapid Communications


In order to assess the presence of anxiolytic properties in cannabidiol (CBD) the drug was tested in an elevated plus-maze model of anxiety, in rats. Doses of 2.5, 5.0 and 10.0 mg/kg significantly increased the entry ratio (open/total number of entries), an anxiolytic-like effect. CBD at a dose of 20.0 mg/kg was no longer effective. None of the doses of CBD used changed total number of entries, a measure of total exploratory activity. Diazepam (2.0 mg/kg) also caused an anxiolytic-like effect in this model. These results indicate that CBD causes a selective anxiolytic effect in the elevated plusmaze, within a limited range of doses.

Key words

Cannabidiol Anxiety Elevated plus-maze 


  1. Cunha JM, Carlini EA, Pereira AE, Ramos OL, Pimentel C, Gagliardi R, Sannito WL, Lander N, Mechoulam R (1980) Chronic administration of cannabidiol to healthy volunteers and epileptic patients. Pharmacology 21:175–185Google Scholar
  2. Dewey WL (1986) Cannabidiol pharmacology. Pharmacol Rev 38:151–178Google Scholar
  3. Grlie L (1976) A comparative study on some chemical and biological characteristics of various samples of cannabis resin. Bull Narc 14:37–46Google Scholar
  4. Pellow S, File SE (1986) Anxiolytic and anxiogenic drug effects on exploratory activity in an elevated plus-maze. A novel test of anxiety in the rat. Pharmacol Biochem Behav 24:525–529Google Scholar
  5. Pellow S, Chopin P, File SE, Briley M (1985) Validation of open: closed arm entries in an elevated plus-maze as a measure of anxiety in the rat. J Neurosci Methods 14:149–167Google Scholar
  6. Silveira Filho NG, Tufik S (1981) Comparative effects between cannabidiol and diazepam on neophobia, food intake and conflict behavior. Res Commun Psychol Psychiatr Behav 6:25–66Google Scholar
  7. Sofia RD, Kubena RK, Barry H (1971) Comparison of four vehicles for intraperitoneal administration of 1-tetrahydrocannabinol. J Pharm Pharmacol 23:889–891Google Scholar
  8. Zuardi AW, Karniol IG (1983) Changes in the conditioned emotional response of rats induced by 9-THC, CBD and mixture of the two cannabinoids. Arq Biol Tecnol 26:391–397Google Scholar
  9. Zuardi AW, Shirakawa I, Finkelfarb E, Karniol IG (1982) Action of cannabidiol on the anxiety and other effects produced by 9-THC in normal subjects. Psychopharmacology 76:245–250Google Scholar

Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • F. S. Guimarães
    • 1
  • T. M. Chiaretti
    • 1
  • F. G. Graeff
    • 3
  • A. W. Zuardi
    • 2
  1. 1.Department of PharmacologyCampus of the University of São PauloRibeirão PretoBrazil
  2. 2.Department of NeuropsychiatryFMRP, Campus of the University of São PauloRibeirão PretoBrazil
  3. 3.Laboratory of Psychobiology, FFCLRPCampus of the University of São PauloRibeirão PretoBrazil

Personalised recommendations