Clinical Rheumatology

, Volume 7, Issue 3, pp 378–383 | Cite as

Anticollagen antibodies in patients with juvenile chronic arthritis

  • J. Svobodová
  • R. Bečvář
  • J. Novotná
  • S. Havelka
  • M. Adam


Using ELISA antibodies, titres to collagen type I, II and III in sera of patients with juvenile chronic arthritis (JCA) were assayed. Results were compared with titres of patients with rheumatoid arthritis (RA). Increased antibodies titres to all three collagen types were found in comparison with healthy individuals of the same age. Average titres in patients with RA were higher than those of the JCA group. When antibodies titres were correlated with erythrocyte sedimentation rate in 1 hour (ESR/1h) statistical significance was found only in RA group for collagen type II.

Key words

Anticollagen Antibodies Juvenile Chronic Arthritis Rheumatoid Arthritis 


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  1. 1.
    Menzel, C., Steffen, C., Kolarz, R., Eberl, R., Frank, O., Thumb, N. Demonstration of antibodies to collagen and of collagen-anticollagen immune complexes in rheumatoid arthritis synovial fluid. Ann Rheum Dis 1976, 35, 446–450.Google Scholar
  2. 2.
    Clague, R.B., Shaw, M.J., Holt, P.J.L. Incidence and correlation between serum IgG and IgM antibodies to native type II collagen in patients with chronic inflammatory arthritis. Ann Rheum Dis 1981, 40, 6–10.PubMedGoogle Scholar
  3. 3.
    Novotna, J., Adam, M. Anticollagen antibodies in patients with osteoarthrosis. Conn Tiss Dis. In press.Google Scholar
  4. 4.
    Brewer, E.J., Bass, J., Baum, J., Current proposed revision of JRA criteria. Arthritis Rheum 1977, 20, 195–199.PubMedGoogle Scholar
  5. 5.
    Arnett, F.C., Edworthy, S., Bloch, D.A., McShane, D.S., Fries, J.F. and the ARA Rheumatoid Arthritis Criteria Subcommittee: The 1987 Revised ARA Criteria for Rheumatoid Arthritis. 51st Annual Meeting of ARA Proc., Washington, 1987, S17.Google Scholar
  6. 6.
    Adam, M., Fietzek, P., Kuhn, K. Investigation on the reaction of metals with collagen in vivo. 2. The formation of cross-links in the collagen of lathyric rats after gold treatment in vivo. Eur J Biochem 1968, 3, 411–417.CrossRefPubMedGoogle Scholar
  7. 7.
    Deyl, Z., Adam, M., Preparation of insoluble collagen. In: The Methodology of Connective Tissue Research, Editor: Hall D.A., Oxford, Joynson-Bruvves Ltd. 1976, 1–23.Google Scholar
  8. 8.
    Adam, M., Deyl, Z., Macek, K. Ways of collagen separation in the pathologically altered tissue. J Chromatogr 1979, 162, 136–142.Google Scholar
  9. 9.
    Laemmli, V.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970, 173, 680–687.CrossRefGoogle Scholar
  10. 10.
    Engwall, E., Perlmann, P. Enzyme-linked immunosorbent assay (ELISA). Quantitative assay of immunoglobulin G. Immunochemistry 1971, 8, 871–874.CrossRefPubMedGoogle Scholar
  11. 11.
    Rosenberg, A.M., Hunt, D.W.C., Petty, R.E., Antibodies to native type I collagen in childhood rheumatic diseases. J Rheumatol 1984, 11, 421–424.PubMedGoogle Scholar
  12. 12.
    Rosenberg, A.M., Hunt, D.W.C., Petty, R.E. Antibodies to native and denatured type II collagen in children with rheumatic diseases. J Rheumatol 1984, 11, 425–431.PubMedGoogle Scholar
  13. 13.
    Petty, R.E., Hunt, D.W.C., Rosenberg, A.M. Antibodies to type IV collagen in rheumatic diseases. J Rheumatol 1986, 13, 246–253.PubMedGoogle Scholar
  14. 14.
    Danieli, M.G., Gabrielli, A., Caniglia, M.L., DeLustro, F., Giorgi, P.L. Antibodies to type II collagen in juvenile rheumatoid arthritis. XI European Congress of Rheumatology Proc., Athens, 1987, 18.Google Scholar
  15. 15.
    Trentham, D.E., Townes, A.S., Kang, A.H., David, J.R. Humoral and cellular sensitivity to collagen in type II collagen-induced arthritis in rats. J Clin Invest 1978, 61, 89–96.PubMedGoogle Scholar
  16. 16.
    Trentham, D.E. Collagen arthritis as a relevant model for rheumatoid arthritis. Arthritis Rheum 1982, 25, 911–916.PubMedGoogle Scholar
  17. 17.
    Boissier, M.C., Feng, X.Z., Carlioz, A., Rondier, R., Fournier, C. Experimental autoimmune arthritis in mice. I. Homologous type II collagen is responsible for self-perpetuating chronic polyarthritis. Ann Rheum Dis 1987, 46, 691–700.PubMedGoogle Scholar
  18. 18.
    Wooley, P.H., Luthra, H.S., Singh, S.K., Huse, A.R., Stuart, S.M., David, C.S. Passive transfer of arthritis to mice by injection of human anti-type II collagen antibody. Mayo Clin Proc 1984, 59, 737–743.PubMedGoogle Scholar
  19. 19.
    Watson, W.C., Cremer, M.A., Wooley, P.H., Townes, A.S. Assessment of the potential pathogenicity of type II collagen autoantibodies in patients with rheumatoid arthritis. Evidence of restricted IgG3 subclass expression and activation of complement C5 to C5a. Arthritis Rheum 1986, 29, 1316–1321.PubMedGoogle Scholar
  20. 20.
    Goto, M., Miysaka, N., Nishioka, K. Stimulation of interleukin 1 release from human monocytes by CB peptides of type II collagen. 51st Annual Meeting of ARA Proc., Washington, 1987, S 58.Google Scholar

Copyright information

© Acta Medica Belgica 1988

Authors and Affiliations

  • J. Svobodová
    • 1
  • R. Bečvář
    • 1
  • J. Novotná
    • 1
  • S. Havelka
    • 1
  • M. Adam
    • 1
  1. 1.Research Institute for Rheumatic DiseasesPraha 2Czechoslovakia

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