Journal of Oceanography

, Volume 49, Issue 5, pp 571–580 | Cite as

Simplified technique for the rapid determination of phytoplankton pigments by reverse-phase high-performance liquid chromatography

  • Reiko Suzuki
  • Masayuki Takahashi
  • Ken Furuya
  • Takashi Ishimaru
Article

Abstract

A simplified technique for the rapid analysis of photosynthetic pigments in marine phytoplankton is described. The pigments extracted in N,N-dimethylformamide (DMF) are injected directly into the reverse-phase high-performance liquid chromatograph fitted with a photodiode array detector. We developed a new stepwise program; that is methanol-water (75∶25 v/v), methanol, methanol-acetone (80∶20) and methanol-acetone (65∶35). This program gives a good separation of at least 13 carotenoids, and 10 chlorophylls and their derivatives, completed within 20 min. The quantitative precision is high; the maximum standard error was less than 6%. The stability of pigments in DMF were also considered for application of this technique to the lab work on board the ship.

Keywords

Methanol Chromatography Chlorophyll Phytoplankton Liquid Chromatograph 

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Copyright information

© Journal of the Oceanographic Society of Japan 1993

Authors and Affiliations

  • Reiko Suzuki
    • 1
  • Masayuki Takahashi
    • 2
  • Ken Furuya
    • 3
  • Takashi Ishimaru
    • 4
  1. 1.Ocean Research InstituteUniversity of TokyoNakano, TokyoJapan
  2. 2.Botany Department, Faculty of ScienceUniversity of TokyoTokyoJapan
  3. 3.Faculty of BioresourcesMie UniversityTsuJapan
  4. 4.Tokyo University of FisheriesMinato, TokyoJapan

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