Journal of Oceanography

, Volume 49, Issue 5, pp 571–580 | Cite as

Simplified technique for the rapid determination of phytoplankton pigments by reverse-phase high-performance liquid chromatography

  • Reiko Suzuki
  • Masayuki Takahashi
  • Ken Furuya
  • Takashi Ishimaru


A simplified technique for the rapid analysis of photosynthetic pigments in marine phytoplankton is described. The pigments extracted in N,N-dimethylformamide (DMF) are injected directly into the reverse-phase high-performance liquid chromatograph fitted with a photodiode array detector. We developed a new stepwise program; that is methanol-water (75∶25 v/v), methanol, methanol-acetone (80∶20) and methanol-acetone (65∶35). This program gives a good separation of at least 13 carotenoids, and 10 chlorophylls and their derivatives, completed within 20 min. The quantitative precision is high; the maximum standard error was less than 6%. The stability of pigments in DMF were also considered for application of this technique to the lab work on board the ship.


Methanol Chromatography Chlorophyll Phytoplankton Liquid Chromatograph 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Journal of the Oceanographic Society of Japan 1993

Authors and Affiliations

  • Reiko Suzuki
    • 1
  • Masayuki Takahashi
    • 2
  • Ken Furuya
    • 3
  • Takashi Ishimaru
    • 4
  1. 1.Ocean Research InstituteUniversity of TokyoNakano, TokyoJapan
  2. 2.Botany Department, Faculty of ScienceUniversity of TokyoTokyoJapan
  3. 3.Faculty of BioresourcesMie UniversityTsuJapan
  4. 4.Tokyo University of FisheriesMinato, TokyoJapan

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