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Diseases of the Colon & Rectum

, Volume 43, Issue 8, pp 1107–1115 | Cite as

Electrocautery snare resection stimulates cellular proliferation of residual colorectal tumor

An increasing gene expression related to tumor growth
  • Masaki Kunihiro
  • Shinji Tanaka
  • Ken Haruma
  • Yasuhiko Kitadai
  • Masaharu Yoshihara
  • Koji Sumii
  • Goro Kajiyama
  • Masahiko Nishiyama
Original Contributions
  • 34 Downloads

Abstract

PURPOSE: Recently, endoscopic mucosal resection has been performed commonly for colorectal tumors. However, incomplete endoscopic mucosal resection produces a residual tumor that grows rapidly. The aim of this study was to clarify the characteristics of the residual tumor using the nude mouse model. METHODS: Human colon cancer cells (colo201 or colo320DM) were implanted subcutaneous into nude mice. We then removed more than one-half of the tumor with an electrocautery snare or a surgical knife, and compared the tumor growth rate with that of control tumors. Before and after resection, we examined the Ki-67 labeling index of the tumors with an immunohistochemical assay and mRNA expression for epidermal growth factor receptor, vascular endothelial growth factor, and transforming growth factor alpha. RESULTS: Residual tumors showed a higher growth rate in tumor volume than control tumors using both methods (electrocautery snare and surgical knife). Colo201 groups showed a higher total volume change per day than colo320DM groups after resection. Furthermore, these tumors also showed a higher Ki-67 labeling index, and a stronger epidermal growth factor receptor and transforming growth factor alpha mRNA expression than primary and control tumors in the colo201 implanted groups. There was no significant difference in vascular endothelial growth factor mRNA expression between groups implanted with colo201 or colo320DM. CONCLUSION: Our results suggest that residual tumors caused by incomplete endoscopic mucosal resection may have a higher growth potential than the tumors before resection.

Key words

Residual colorectal cancer Cellular proliferative activity Endoscopic treatment 

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Copyright information

© The American Society of Colon and Rectal Surgeons 2000

Authors and Affiliations

  • Masaki Kunihiro
    • 1
  • Shinji Tanaka
    • 3
  • Ken Haruma
    • 1
  • Yasuhiko Kitadai
    • 3
  • Masaharu Yoshihara
    • 1
  • Koji Sumii
    • 1
  • Goro Kajiyama
    • 1
  • Masahiko Nishiyama
    • 2
  1. 1.From the First Department of Internal MedicineHiroshima University School of MedicineHiroshimaJapan
  2. 2.the Department of Biochemistry and Biophysics, Division of Molecular Biology, Research Institute for Radiation Biology and MedicineHiroshima UniversityHiroshimaJapan
  3. 3.Department of EndoscopyHiroshima University School of MedicineHiroshimaJapan

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