Advertisement

Diseases of the Colon & Rectum

, Volume 42, Issue 10, pp 1334–1338 | Cite as

Technical manual for manufacturing autologous fibrin tissue adhesive

  • John J. Park
  • Jose R. Cintron
  • Karl H. Siedentop
  • Charles P. Orsay
  • Russell K. Pearl
  • Richard L. Nelson
  • Herand Abcarian
Original Contributions
  • 29 Downloads

Abstract

PURPOSE: The aim of this article is to provide a concise and simple technical manual for manufacturing autologous fibrin tissue adhesive derived from the precipitation of fibrinogen using a combination of ethanol and freezing for surgery. METHODS: All materials and equipment needed to manufacture ethanol-based autologous fibrin tissue adhesive are listed. In addition, step-by-step instructions are provided to allow for easy and rapid fibrin adhesive production. RESULTS: Ethanol-based autologous fibrin tissue adhesive can be manufactured in under 60 minutes. Furthermore, at our institution the startup cost for manufacturing ethanol-based autologous fibrin tissue adhesive was under $2,500.00. CONCLUSION: Ethanol-based autologous fibrin tissue adhesive is a safe, reliable, and easily manufactured autologous fibrin tissue adhesive that can be made by a trained technician in any blood bank, pharmacy, or surgical laboratory.

Key words

Fistula Autologous fibrin glue 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Park JJ, Siedentop KH, Chung S, Sanchez B, Bhattacharyya T. Comparison of the bonding power of various autologous fibrin tissue adhesives. Am J Otol 1997;18:655–9.PubMedGoogle Scholar
  2. 2.
    Siedentop KH, Harris DM, Sanchez B. Autologous fibrin tissue adhesive. Laryngoscope 1985;95:1074–6.PubMedGoogle Scholar
  3. 3.
    Harris DM, Siedentop KH, Ham KR, Sanchez B. Autologous fibrin tissue adhesive: biodegradation and systemic effects. Laryngoscope 1987;97:1141–4.PubMedGoogle Scholar
  4. 4.
    Siedentop KH, Park JJ, Sanchez B. An autologous fibrin tissue adhesive with greater bonding power. Arch Otolaryngol Head Neck Surg 1995;121:769–72.PubMedGoogle Scholar
  5. 5.
    Weis-Fogh US. Fibrinogen prepared from small blood samples for autologous use in a tissue adhesive system. Eur Surg Res 1988;20:381–9.PubMedGoogle Scholar
  6. 6.
    Dahlstrom KK, Weis-Fogh US, Medgyesi S, Rostgaard J, Sorensen H. The use of autologous fibrin adhesive in skin transplantation. Plast Reconstr Surg 1992;89:968–72.PubMedGoogle Scholar
  7. 7.
    Kjaergard HK, Weis-Fogh US. Autologous fibrin glue for sealing vascular prostheses of high porosity. Cardiovasc Surg 1994;2:45–7.PubMedGoogle Scholar
  8. 8.
    Siedentop KH, Chung SE, Park JJ, Sanchez B, Bhattacharya T, Marx G. Evaluation of pooled fibrin sealant for ear surgery. Am J Otol 1997;18:660–4.PubMedGoogle Scholar
  9. 9.
    Cintron JR, Park JJ, Orsay CP, Pearl RK, Nelson RL, Abcarian H. Repair of fistulas in ano using autologous fibrin tissue adhesive. Dis Colon Rectum 1999;42:607–13.PubMedGoogle Scholar
  10. 10.
    Christie RJ, Carrington L, Alving B. Postoperative bleeding induced by topical bovine thrombin: report of two cases. Surgery 1997;121:708–10.PubMedGoogle Scholar

Copyright information

© The American Society of Colon and Rectal Surgeons 1999

Authors and Affiliations

  • John J. Park
    • 1
  • Jose R. Cintron
    • 1
    • 2
  • Karl H. Siedentop
    • 1
  • Charles P. Orsay
    • 2
  • Russell K. Pearl
    • 3
  • Richard L. Nelson
    • 1
  • Herand Abcarian
    • 1
  1. 1.From the Department of SurgeryThe University of Illinois Hospital and ClinicsUSA
  2. 2.Department of OtolaryngologyThe University of Illinois Hospital and ClinicsUSA
  3. 3.Department of Surgery, Division of Colon and Rectal SurgeryCook County HospitalChicago

Personalised recommendations