Skip to main content

Effect of vagotomy and/or antrectomy on gastric secretion stimulated by intravenous infusion of bethanechol chloride

Study on rats with gastric fistula

The American Journal of Digestive Diseases volume 16, pages 19–26 (1971)Cite this article

Abstract

The interdigestive phase of gastric secretion was investigated for 24 hr in rats bearing permanent gastric fistulas. Intact controls, vagotomized, antrectomized and vagotomized-antrectomized animals were studied. Vagotomy, alone or combined with antrectomy, abolished the secretory function of parietal and zymogenic cells during the spontaneous interdigestive phase of secretion. This was considered due to the fact that the interdigestive secretion is vagus dependent and represents a true cephalic phase. Antrectomy did not result in the decrease of spontaneous secretion of HCl or pepsin. Intra-arterial stimulation with a stable choline ester, bethanechol, produced adequate secretory response in intact rats and partially restored the function of parietal cells in vagotomized rats. The action of bethanechol on HCl secretion in antrectomized rats was less marked. However, bethanechol has a very marked stimulatory effect on the secretion of pepsin in intact, vagotomized and antrectomized rats. Sensitivity of gastric mucosa to bethanechol was nearly abolished in vagotomized-antrectomized rats.

It appears from this experiment that interdigestive, spontaneous secretion of HCl and pepsin in rats is vagus dependent. Under the conditions of the present study, no action of endogenous gastrin was demonstrated during the interdigestive phase of gastric secretion.

This is a preview of subscription content, access via your institution.

Access options

Buy single article

Instant access to the full article PDF.

43,34 €

Price includes VAT (Finland)
Tax calculation will be finalised during checkout.

References

  1. Grossman MI: Physiological role of gastrin. Fed Proc 27:1312, 1968

    PubMed  Google Scholar 

  2. Gregory RA: Secretory mechanisms of the digestive tract. Annual Rev Physiol 27:395, 1965

    Article  PubMed  Google Scholar 

  3. Schofield B, Redford M, Grabham AH, et al: Neural factors in the control of gastrin release, Gastric Secretion, Mechanisms and Control. Edited by TK Shnitka, JAL Gilbert, RC Harrison. Oxford, Pergamon Press, 1967, p 91

    Google Scholar 

  4. Grossman ME: Some physiological actions of gastrin, Gastric Secretion, Mechanisms and Control. Edited by TK Shnitka, JAL Gilbert, RC Harrison. Oxford, Pergamon Press, 1967, p 317

    Google Scholar 

  5. Griffen WO Jr: Antral regulatory mechanism—an hypothesis. Surg Clin North Amer 47:1335, 1967

    Google Scholar 

  6. Peskin GW, Thompson JC: The gastric antrum. Amer J Med Sci 239:151, 1960

    Google Scholar 

  7. Robertson CR, Langlois K, Martin CG, et al: Release of gastrin in response to bathing the pyloric mucosa with acetylcholine. Amer J Physiol 163:27, 1950

    PubMed  Google Scholar 

  8. Redford M, Schofield B: The effect of local anaesthesia of the pyloric antral mucosa on acid inhibition of gastrin-mediated acid secretion. J Physiol 180:304, 1965

    PubMed  Google Scholar 

  9. Morton GM, Stavraky GW: A histo-physiological study of the effect of intra-arterial injection of acetylcholine upon the gastric mucosa of the dog. Gastroenterology 12:808, 1949

    Google Scholar 

  10. Redford M, Schofield B: Inhibition of the acid response to urecholin by antral acidification in dogs. J Physiol 153:70P, 1960

    Google Scholar 

  11. Passaro EP, Jr, Grossman MI: Effect of vagal innervation on acid and pepsin response to histamine and gastrin. Amer J Physiol 206:1068, 1964

    PubMed  Google Scholar 

  12. Emas S, Grossman MI: Difference in responses between dogs and cats to large doses of gastrin on gastric secretion. Gut 8:267, 1967

    PubMed  Google Scholar 

  13. Bennett A, Hogbin BM: The effect of gastrin on secretion of acid by the rat stomach. J Physiol 198:91P, 1968

    Google Scholar 

  14. Adashek K, Grossman MI: Response of rats with gastric fistulas to injection of gastrin. Proc Soc Exp Biol Med 112:629, 1963

    PubMed  Google Scholar 

  15. Legros G, Griffith CA: The abdominal vagal system in rats: an anatomical study with emphasis upon the distribution of the gastric vagi to the stomach. J Surg Res 9:183, 1969

    Article  PubMed  Google Scholar 

  16. Komarov SA, Shay H, Rayport M, et al: Some observations on gastric secretion in normal rats. Gastroenterology 3:406, 1944

    Google Scholar 

  17. Alphin RS, Lin TM: Preparation of chronic denervated gastric pouches in the rat. Amer J Physiol 197:257, 1959

    PubMed  Google Scholar 

  18. Lin TM, Alphin RS: Cephalic phase of gastric secretion in the rat. Amer J Physiol 192:23, 1958

    PubMed  Google Scholar 

  19. Shay H, Komarov SA, Gruenstein M: Effects of vagotomy in the rat. AMA Arch Surg 59:210, 1949

    Google Scholar 

  20. Lambert R: Surgery of the Digestive System in the Rat. Charles C Thomas, Springfield, 1965

    Google Scholar 

  21. Kowalewski K, Chmura G: A method permitting prolonged and repeated studies of rat's gastric secretion. Arch Int Physiol Biochim 77:10, 1969

    PubMed  Google Scholar 

  22. Kowalewski K, Chmura G: Determination of histamine dose causing maximal gastric secretion: study in rats with gastric fistulas. Amer J Dig Dis 13:753, 1968

    Article  PubMed  Google Scholar 

  23. Kowalewski K, Chmura G, Schier J: Experimental deficiency of gastric “mucus barrier.” Its effect on gastric secretion and on the formation of acute gastric ulcer in rats infused with histamine for 24 hours. Amer J Dig Dis 14:788–796, 1969

    PubMed  Google Scholar 

  24. Bollman JL: A cage which limits the activity of rats. J Lab Clin Med 33:1348, 1948

    Google Scholar 

  25. Anson MI: The estimation of pepsin, trypsin, papain and cathepsin with hemoglobin. J Gen Physiol 22:79, 1938

    Article  Google Scholar 

  26. Aitken MA, Spray GH, Walters G: Gastric pepsin and the excretion of uropepsinogen in anemia. Clin Sci 13:119, 1954

    PubMed  Google Scholar 

  27. Bralow SP, Komarov SA: Parietal cell mass and distribution in stomachs of Wistar rats. Amer J Physiol 203:550, 1962

    PubMed  Google Scholar 

  28. Baron JH, Burrows L, Wildstein W, et al: The effect of antrectomy and vagotomy on maximally stimulated canine Heidenhain pouches. Amer J Gastroent 44:467, 1965

    PubMed  Google Scholar 

  29. Ritchie WP, Cheng JWB, Delaney JP: Changes in parietal and chief cell populations following vagotomy and antrectomy. Surgical Forum 20:319, 1969

    PubMed  Google Scholar 

  30. Bunch WH, Ott G, Goodale RL, et al: Changes in composition of gastric cellular membranes after vagotomy. Surg Forum 20:320, 1969

    PubMed  Google Scholar 

  31. Jordan PH Jr, De La Rosa C: Magnitude of the antrum's role in the cephalic phase of gastric secretion, Gastric Secretion, Mechanisms and Control. Edited by TK Shnitka, JAL Gilbert, RC Harrison. Oxford, Pergamon Press, 1967, p 119

    Google Scholar 

  32. Olbe L: Vagal effects on gastric secretion, Gastric Secretion Mechanisms and Control. Edited by TK Shnitka, JAL Gilbert, RC Harrison. Oxford, Pergamon Press, 1967, p 83

    Google Scholar 

  33. Andersson S, Grossman MI: Effect of antrectomy on gastric secretion of acid and pepsin in response to histamine and gastrin in dogs. Gastroenterology 49:246, 1965

    PubMed  Google Scholar 

  34. Andersson S, Grossman MI: Effect of vagal denervation of pouches on gastric secretion in dogs with intact or resected antrums. Gastroenterology 48:449, 1965

    PubMed  Google Scholar 

  35. De La Rosa C, Linares CA, Woodward ER, et al: Effect of vagotomy on the gastric secretory response to endogenous gastrin. Arch Surg 93:583, 1966

    PubMed  Google Scholar 

Download references

Author information

Affiliations

  1. From the Surgical-Medical Research Institute, The University of Alberta, Edmonton, Alberta, Canada

    K. Kowalewski PhD, MD

Authors
  1. K. Kowalewski PhD, MD
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

The technical assistance of Mr. G. Chmura, Mrs. T. Hoogen, Mrs. M. McCubbin and Mr. T. Pachkowski is gratefully acknowledged; Merck Sharp and Dohme of Canada Limited, Montreal, Canada, is thanked for the supply of Bethanechol chloride (Urecholine).

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Kowalewski, K. Effect of vagotomy and/or antrectomy on gastric secretion stimulated by intravenous infusion of bethanechol chloride. Digest Dis Sci 16, 19–26 (1971). https://doi.org/10.1007/BF02233784

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02233784

Keywords

  • Choline
  • Parietal Cell
  • Gastric Secretion
  • Secretory Response
  • Secretory Function