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Proteolytic activity of human gastric juices at various pH's and its estimation by hemoglobin and electrophoretic methods

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References

  1. Schwann, T. Über das Wesen des Verdaungsprozesses.Arch. Anat. Physiol. Wiss. 90, 1836.

  2. Northrop, J. H. Crystalline pepsin: Isolation and tests of purity.J. Gen. Physiol. 13:739, 1929–1930.

    Google Scholar 

  3. Northrop, J. H. Crystalline pepsin.II. General properties and experimental methods. J. Gen. Physiol. 13:767, 1929–1930.

    Google Scholar 

  4. Van Vunakis, H., andLevine, L. Structural studies on pepsinogen and pepsin: An immunological approach.Ann. New York Acad. Sc. 103:735, 1963.

    Google Scholar 

  5. Arnon, R., andPerlman, G. E. Antibodies to pepsinogen and pepsinogen modification.Ann. New York Acad. Sc. 103:744, 1963.

    Google Scholar 

  6. Taylor, W. H. Studies on gastric proteolysis.1. The proteolytic activity of human gastric juice and pig and calf gastric mucosal extracts below pH 5. Biochem J. 71: 73, 1959.

    Google Scholar 

  7. Taylor, W. H. Studies on gastric proteolysis.2. The nature of the enzymesubstrate interaction responsible for gastric proteolytic pH-activity curves with two maxima. Biochem. J. 71: 373, 1959.

    Google Scholar 

  8. Taylor, W. H. Studies on gastric proteolysis.3. The secretion of different pepsins by fundic and pyloric glands of the stomach. Biochem. J. 71:384, 1959.

    Google Scholar 

  9. Taylor, W. H. Proteinases of the stomach in health and disease.Physiol. Rev. 42: 519, 1962.

    PubMed  Google Scholar 

  10. Richmond, V., Tang, J., Wolf, S., Trucco, R. E., andCaputto, R. Chromatographic isolation of gastricsin, the proteolytic enzyme from gastric juice with pH optimum 3.2. Biochim. et biophys. acta. 29:453, 1958.

    Google Scholar 

  11. Tang, J., andTang, K. I. Purification and properties of a zymogen from human gastric mucosa.J. Biol. Chem. 238:606, 1963.

    PubMed  Google Scholar 

  12. Tang, J., andWolf, S. Gastricsin (Editorial).Gastroenterology 44:908, 1963.

    Google Scholar 

  13. Tang, J., Wolf, S., Caputto, R., andTrucco, R. E. Isolation and crystallization of gastricsin from human gastric juice.J. Biol. Chem. 234:1174, 1959.

    PubMed  Google Scholar 

  14. Seijffers, M. J., Miller, L. L., andSegal, H. L. The separation of pepsins from human gastric juice.J. A. M. A. 183: 998, 1963.

    PubMed  Google Scholar 

  15. Seijffers, M. J., Segal, H. L., andMiller, L. L. Separation of 3 pepsinogens from human gastric mucosa.Am. J. Physiol. 205: 1099, 1963.

    PubMed  Google Scholar 

  16. Seijffers, M. J., Miller, L. L., andSegal, H. L. Some observations on the conversion of three different human pepsinogens to their respective pepsins.Biochemistry 3:1, 1964.

    PubMed  Google Scholar 

  17. Hirsch-Marie, H., andBurtin, P. Analyse immunoélectrophorétique des liquides gastriques normaux et pathologiques. InProtides Biol. Fluids, Proc. Colloq. Brugge. 1936. Elsevier, Amsterdam, 1964, p. 256.

    Google Scholar 

  18. Hirsch-Marie, H., Burtin, P., and Conte, M. Étude électrophorétique et immunoélectrophor étique des enzymes proteolytiques du suc gastrique normal et pathologique. 7th Congr. Internat. de l'Assoc. Soc. Nat. Europ. & Méditerran. d. Gastroenterologie. Bruxelles, June 1–6, 1964,Resumés des Communications, p. 3 (Abstract) , 1964.

  19. Kushner, I., Rapp, W., andBurtin, P. Separation and characterization of immunologically active constituents of normal human gastric mucosa.Protides Biol. Fluids, Proc. Colloq. Brugge, 1963. Elsevier, Amsterdam, 1964, p. 248.

    Google Scholar 

  20. Rapp, W., Aronson, S. B., andBurtin, P. Étude comparative des proteines de la muqueuse gastrique humaine normale et cancéreuse par électrophorése et immunoélectrophorése.Protides Biol. Fluids, Proc. Colloq. Brugge, 1963. Elsevier, Amsterdam, 1964, p. 252.

    Google Scholar 

  21. Freudenberg, E. Über das Kathepsin des Magensaftes.Enzymologia 8:385, 1940.

    Google Scholar 

  22. Freudenberg, E., andBuchs, S. Über die zweite Protease des Magensaftes, das Kathepsin.Schweiz. med. Wochnschr. 70: 249, 1940.

    Google Scholar 

  23. Buchs, S. Die Biologie des Magenkathepsins. Karger, Basel, 1947.

    Google Scholar 

  24. Buchs, S. Pepsin, cathepsin and parachymosin as equivalent and integrant constituents of stomach protease.Enzymologia 13:208, 1949.

    Google Scholar 

  25. Buchs, S. Fundamental observations on the existence, extraction and activation of gastric cathepsin.Enzymologia 16:193, 1953.

    PubMed  Google Scholar 

  26. Buchs, S. Weitere Untersuchungen uber die Extraktionsarten und die Stabilität der Magenprotease.Ztschr. Vitamin-Hormonu. Fermentforsch. 6:245, 1954.

    Google Scholar 

  27. Merten, R., andRatzer, H. Zur Charakterisierung des Magen- und Harnkathepsins des Erwachsenen.Klin. Wchnschr. 27:587, 1949.

    Google Scholar 

  28. Miller, L. L., Segal, H. L., andPlumb, E. J. Proteolytic enzyme activity. II. Gastric and urinary proteolytic activities at pH 1.5 and 3.5.Gastroenterology 33:566, 1957.

    PubMed  Google Scholar 

  29. Ryle, A. P. Parapepsinogen. II. The zymogen of parapepsin II.Biochem. J. 75: 145, 1960.

    PubMed  Google Scholar 

  30. Ryle, A. P., andPorter, R. R. Parapepsins; two proteolytic enzymes associated with porcine pepsin.Biochem. J. 73:75, 1959.

    PubMed  Google Scholar 

  31. Merten, R., Schramm, G., Grassmann, W., andHannig, K. Untersuchungen zur Reindarstellung des Magenkathepsins.Z. Physiol. Chem. 289:173, 1952.

    Google Scholar 

  32. Grossberg, A. L., Komarov, S. A., andShay, H. Mucoproteins of gastric juice. Electrophoretic characterization and separation of fractions.Am. J. Physiol. 165:1, 1951.

    PubMed  Google Scholar 

  33. Heinrich, W. D. Über Papierelektrophorese von Pepsin.Biochem. Ztschr. 323:469, 1953.

    Google Scholar 

  34. Norpoth, L., Surmann, T., andClösges, J. Über den Ort der Magenfermente im Elcktrophoresediagramm.Klin. Wchnschr. 31: 1005, 1953.

    Google Scholar 

  35. Gräsbeck, R. Studies on the vitamin B12-binding principle and other biocolloids of human gastric juice.Acta med. scandinav. Suppl. 314:1, 1956.

    Google Scholar 

  36. Horowitz, M. I., andHollander, F. Serum proteins in anacid gastric mucinous secretion.J. Biol. Chem. 236:770, 1961.

    PubMed  Google Scholar 

  37. Jeffries, G. H., Smith, F. W., Hoskins, D. W., andSleisenger, M. H. Fractionation of the non-dialyzable components of normal human gastric juice by starch gel electrophoresis.Gastroenterology 41: 467, 1961.

    PubMed  Google Scholar 

  38. Glass, G. B. J. Paper electrophoresis of gastric juice in health and disease.Am. J. Digest. Diseases 6:1131, 1961; In “Current Gastroenterology” (G. McHardy, ed.), pp. 90–151. Harper (Hoeber) , New York, 1962.

    Google Scholar 

  39. Glass, G. B. J., Stephanson-Liounis, L., Rich, M., andMitchell, S. E. Quantitation of pepsin in gastric juice by paper electrophoresis.(Abst.) Fed. Proc. 17: 54, 1958.

    Google Scholar 

  40. Ivy, A. C. Studies on the secretion of the pyloric end of the stomach.Am. J. Physiol. 49:142, 1919.

    Google Scholar 

  41. Linderstrom-Land, K., andHolter, H. Studies on enzymatic histochemistry. XI. The distribution of peptidase in the gastric and duodenal mucosa of the pig.Compt. Rend. Trav. Lab. Carlsberg 20:42, 1935.

    Google Scholar 

  42. Lim, R. K. S., andDott, N. M. Observations on the isolated pyloric segment and on its secretion.Quart. J. Exper. Physiol. 13: 159, 1923.

    Google Scholar 

  43. Meulengracht, E., andSchiodt, E. Pepsin and renin activity of preparations of dried stomach from cardia, fundus and pyloris respectively.Acta med. scandinav. 82:375, 1934.

    Google Scholar 

  44. Grossman, M. I., andMarks, I. N. Secretion of pepsinogen by the pyloric glands of the dog, with some observations on the histology of the gastric mucosa.Gastroenterology 38:343, 1960.

    PubMed  Google Scholar 

  45. Glass, G. B. J., Pugh, B. L., andWolf, S. A new modification of the hemoglobin technic for the determination of pepsin in gastric juice adapted for a wide range of valucs.Rev. Gastroenterol. 18:670, 1951.

    PubMed  Google Scholar 

  46. Glass, G. B. J., Stephanson, L., andRich, M. Paper-electrophoretic analysis of gastric juice in health and disease and its physiological and clinical significance.Gastroenterologia 86:384, 1956.

    PubMed  Google Scholar 

  47. Castro-Curel, Z., andGlass, G. B. J. Fractionation of intrinsic factor and proteolytic enzymes of human gastric mucosa on DEAE-cellulose column.Fed. Proc. 23: 439, 1964.

    Google Scholar 

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Authors and Affiliations

  1. From the Gastroenterology Research Laboratory and Section of Gastroenterology, Department of Medicine, New York Medical College-Metropolitan Hospital Medical Center, New York, N. Y.

    George B. Jerzy Glass M.D. & Akira Ishimori M.D.

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  1. George B. Jerzy Glass M.D.
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  2. Akira Ishimori M.D.
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  3. Marilyn Rich A.B.
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Additional information

Supported by Research Grant-in-Aid AM-00068 (10–12) from the National Institute of Arthritis and Metabolic Diseases, U. S. Public Health Service, and grant P-306 (C-E) from the American Cancer Society.

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Glass, G.B.J., Ishimori, A. & Rich, M. Proteolytic activity of human gastric juices at various pH's and its estimation by hemoglobin and electrophoretic methods. Digest Dis Sci 10, 766–789 (1965). https://doi.org/10.1007/BF02233770

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