The American Journal of Digestive Diseases

, Volume 13, Issue 8, pp 753–761 | Cite as

Determination of histamine dose causing maximal gastric secretion

Study in rats with gastric fistulas
  • K. Kowalewski
  • G. Chmura


1. Adult male rats with permanent gastric fistulas were infused intravenously for 24 hr. with histamine dihydrochloride in normal saline. The infusion rate was 100 ml./kg./24 hr.; doses of histamine tested in this study were 5, 10, 25, 50, 75, 100, 125, 250, 500, 1000, 1500, and 2000 mg./kg./24 hr.

2. Peak response in the output of gastric HCl and pepsin occurred with the dose of histamine 100 mg./kg./24 hr. and was due to the large volume of secretion obtained at this dosage level.

3. Concentrations of HCl and pepsin were essentially the same in the rats infused with various doses of histamine; no dose-response curve was observed under the conditions of this study.

4. A remarkable absence of fatigue of gastric parietal and chief cells during prolonged stimulation with histamine is discussed in view of the results of the results of the present experiment.


Fatigue Histamine Present Experiment Large Volume Dosage Level 
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  1. 1.
    Conard, V., Kowalewski, K., andVan Geertruyden, J. Action de doses croissantes d'histamine sur la sécrétion gastrique chez des malades protégés par l'antistine.Acta Gastroent Belg 12:97, 1949.Google Scholar
  2. 2.
    Kowalewski, K., Conard, V., andVan Geertruyden, J. Réactions de la muqueuse gastrique à des doses élévees d'histamine: Achlorhydrie absolue et achlorhydrie relative.C R Soc Biol (Paris) 144:291, 1950.Google Scholar
  3. 3.
    Roe, J. H., andDyer, H. M. Relation of nutrition to gastric function: I. An experimental method.Proc Soc Exp Biol Med 41:603, 1939.Google Scholar
  4. 4.
    Kyle, J., andWelbourne, R. B. The effect of histamine on gastric secretion and ulceration in the pylorus ligated rat.Gastroenterology 30:593, 1956.PubMedGoogle Scholar
  5. 5.
    Lane, A., Ivy, A. C., andIvy, E. K. Response of the chronic gastric fistula rat to histamine.Amer J Physiol 190:221, 1957.PubMedGoogle Scholar
  6. 6.
    Aarsen, P. N., andVan Noordwijk, J. The effect of atropine, propantheline and poldine on the vagally stimulated gastric motility and the histamine-stimulated acid gastric secretion in the rat.Brit J Pharmacol 17:41, 1961.PubMedGoogle Scholar
  7. 7.
    Valberg, L. S., andWitts, L. T. The histamine test meal in the rat.Gut 2:32, 1961.PubMedGoogle Scholar
  8. 8.
    Alphin, R. S., andLin, T. M. Response of chronic denervated gastric pouches of rats to food and histamine.Amer J Physiol 203:641, 1962.PubMedGoogle Scholar
  9. 9.
    Ridley, P. Electrolytes, pepsin and gastric motility after histamine and insulin in chronic fistula rats. (abst.)Fed Proc 22:1088, 1963.PubMedGoogle Scholar
  10. 10.
    Baume, P. E., andLaw, D. H. Effect of histamine on gastric secretion in the pylorus ligated rat.Proc Soc Exp Biol Med 119:461, 1965.PubMedGoogle Scholar
  11. 11.
    Thompson, J. H., andLee, Y. H. Determination of the dose of histamine causing maximal gastric acid secretion in the pylorus-ligated rat.Amer J Dig Dis 12:449, 1967.PubMedGoogle Scholar
  12. 12.
    Kowalewski, K. Effect of infusion of histamine in normal saline and in glucose on gastric secretion in rats with permanent gastric fistulas.Arch Int Physiol 75:450, 1967.PubMedGoogle Scholar
  13. 13.
    Kowalewski, K. Effect of long-term treatment with an anticholinergic drug on gastric secretion in rats.Amer J Dig Dis 12:988, 1967.PubMedGoogle Scholar
  14. 14.
    Ambrus, J. L., Ambrus, C. M., andHarrisson, J. W. E. Gastric acid secretion and gastric ulcer formation of rats and guinea pigs.Amer J Physiol 173:95, 1953.PubMedGoogle Scholar
  15. 15.
    Friedman, M. H. F. Histamine ineffective in the rat as a gastric secretory stimulant.Proc Soc Exp Biol Med 54:42, 1943.Google Scholar
  16. 16.
    Komarov, S. A., Shay, H., Rayport, M., andFels, S. S. Some observations on gastric secretion in normal rats.Gastroenterology 3:406, 1944.Google Scholar
  17. 17.
    Lin, T. M., Alphin, R. S., Henderson, F. G., andChen, K. K. 3-β-Aminoethyl-1,2,4-triazole, a potent stimulant of gastric secretion.J Pharmacol Exp Ther 134:88, 1961.PubMedGoogle Scholar
  18. 18.
    Lozzio, B. B., Blempica, L., Royer, M., andGorodisch, S. Action of aminoguanidine on the stomach of pylorus-ligated rats.Amer J Dig Dis 6:534, 1961.PubMedGoogle Scholar
  19. 19.
    Brodie, D. A., andKnapp, P. G. The mechanism of the inhibition of gastric secretion produced by esophageal ligation in the pylorus-ligated rat.Gastroenterology 50:787, 1966.PubMedGoogle Scholar
  20. 20.
    Brodie, D. A. The mechanism of gastric hyperacidity produced by pylorus ligation in the rat.Amer J Dig Dis 11:231, 1966.PubMedGoogle Scholar
  21. 21.
    Komarov, S. A., Bralow, S. P., andBoyd, E. A permanent rat gastric fistula.Proc Soc Exp Biol Med 112:451, 1963.PubMedGoogle Scholar
  22. 22.
    Adam, H. M., Card, W. I., Riddell, M. J., Roberts, M., Strong, J. A., andWoolf, B. Dose-response curves for the effect of histamine on acid gastric secretion in man.Brit J Pharmacol 9:329, 1954.PubMedGoogle Scholar
  23. 23.
    Wyllie, J. H., andSmith, G. Histamine-infusion test.Lancet 2:823, 1965.PubMedGoogle Scholar
  24. 24.
    Bollman, J. L. A cage which limits the activity of rats.J Lab Clin Med 33:1348, 1948.Google Scholar
  25. 25.
    Kowalewski, K. Importance of dosage of stimulant in histamine test of gastric function.DVA Treatment Serv Bull 8:325, 1953.Google Scholar
  26. 26.
    Anson, M. L. The estimation of pepsin, trypsin, papain and cathepsin with hemoglobin.J Gen Physiol 22:79, 1938.Google Scholar
  27. 27.
    Aitken, M. A., Spray, G. H., andWalters, G. Gastric pepsin and the execretion of uropepsinogen in anemia.Clin Sci 13:119, 1954.PubMedGoogle Scholar
  28. 28.
    Kolthoff, I. M., andSandell, E. B. Textbook of Quantitative Inorganic Analysis. MacMillan, New York, 1952, p. 429.Google Scholar
  29. 29.
    Wilcoxon, F. Individual comparison by ranking methods.Biometrics 1:80, 1945.Google Scholar
  30. 30.
    Kowalewski, K., andBain, G. O. Prevention of post-histaminic gastric ulcers in guinea pigs by posterior pituitary extract.Acta Gastroent Belg 17:539, 1954.PubMedGoogle Scholar
  31. 31.
    Christiansen, P. M. Measurement of gastric acidity.Lancet 2:778, 1967.Google Scholar
  32. 32.
    Lubran, M. Measurement of gastric acidity.Lancet 2:1070, 1966.PubMedGoogle Scholar
  33. 33.
    Hollander, F. The chemistry and mechanics of hydrochloric acid formation in the stomach.Gastroenterology 1:401, 1943.Google Scholar
  34. 34.
    Dritsas, K. G., andKowalewski, K. Perfusion of the isolated canine stomach.Brit J Surg 53:732, 1966.PubMedGoogle Scholar
  35. 35.
    Dritsas, K. G., Bondar, G. F., andKowalewski, K. Secretory response to sustained histamine stimulation of the isolated canine stomach.Brit J Surg 53:798, 1966.PubMedGoogle Scholar
  36. 36.
    Dritsas, K. G., andKowalewski, K. The mechanism of pepsin secretion in the isolated perfused canine stomach.Canad J Surg 11:85, 1968.PubMedGoogle Scholar
  37. 37.
    Kowalewski, K., Bondar, G. F., andUrushibara, O. A method of producing pyloric stenosis in dogs.Canad J Surg 10:483, 1967.PubMedGoogle Scholar

Copyright information

© Hoeber Medical Division • Harper & Row, Publishers, Incorporated 1968

Authors and Affiliations

  • K. Kowalewski
    • 1
  • G. Chmura
    • 1
  1. 1.From the Surgical-Medical Research InstituteUniversity of AlbertaEdmontonCanada

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