Plant and Soil

, Volume 118, Issue 1–2, pp 221–229 | Cite as

Detection of pectolytic activity andpel homologous sequences inFrankia

  • A. Séguin
  • M. Lalonde


Using a cup-plate pectin agar assay, pectolytic activity was detected in nodule filtrates obtained fromAlnus rugosa (DuRoi) Spreng,A. glutinosa (L.) Gaertn andA. crispa (Ait.) Pursh seedlings after infection with twoFrankia strains (ACN1 AG , CpI1). Pectolytic activity was also detected in cultures filtrates of the same twoFrankia isolates afterin vitro-cultivation on Qmod pectin liquid medium. When Southern blots of Frankia total DNAs from 3 isolates ofF. alni subsp.Pommerii (ACN1 AG , ArI3, and CPX32b) and 3 isolates ofF. elaeagni (EUN1 pec, SCN 10a and TX31e HR ) were hybridized withPelBDA probes fromErwinia chrysanthemi, positive signals were found in all 7 Frankiae tested.

Key words

actinorhizal plants DNA-DNA hybridization Frankia pectate lyase pectolytic activity root symbiosis 


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Copyright information

© Kluwer Academic Publishers 1989

Authors and Affiliations

  • A. Séguin
    • 1
  • M. Lalonde
    • 1
  1. 1.Centre de Recherche en Biologie Forestières, Faculté de Foresterie et de GéodésieUniversité LavalCanada

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