Abstract
The present study was undertaken to set up an experimental system in which barriers to infection of a non-host plant related to the presence of the cell wall, at the level of recognition and/or the necessity of penetrating the cell wall, might be bypassed. Co-cultures betweenFrankia alni subsp.pommerii (strain ACN1AG) andBetula papyrifera protoplasts were established. Betula protoplasts remained viable after 2 weeks with no substantial cell wall regeneration. Suppression of the wall barrier was not sufficient to allowFrankia infection under the conditions tested. The non-infectivity ofFrankia on Betula protoplasts may also reflect difficulties inherent to thein vitro environment, which might not permit duplication of infection mechanisms.
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Abbreviations
- 2,4-D:
-
2,4-dichlorophenoxyacetic acid
- NAA:
-
naphtaleneacetic acid
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Laliberté, S., Lalonde, M. Co-culture ofFrankia alni subsp.pommerii (strain ACN1AG) with birch (Betula papyrifera) protoplasts. Plant Soil 118, 189–197 (1989). https://doi.org/10.1007/BF02232806
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DOI: https://doi.org/10.1007/BF02232806