Plant and Soil

, Volume 118, Issue 1–2, pp 139–143 | Cite as

Host plant growth response to inoculation withFrankia

  • D. Bernie Steele
  • K. Ramirez
  • M. D. Stowers


Optimum growth conditions and inoculation regimes were determined for severalFrankia strains isolated from both Alnus and Casuarina host plants. Growth conditions were estabilished that allowed a reduction in generation time to less than 15 hours for certain Alnus derivedFrankia. Differences in plant growth response were observed with differing inoculum levels and soil mixtures. Elite strains of Alnus derivedFrankia were isolated that elicited similar growth reponses in allAlnus species tested; however, differences were observed betweenFrankia strains and plant growth response of variousCasuarina species tested.

Key words

actinorhizal Alnus Casuarina symbiosis 


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  1. Benson D R, Mazzucco C E and Browning T J 1985 Physiological aspects ofFrankia. In Nitrogen Fixation and CO2 Metabolism. Eds. P W Ludden and J E Burris. pp 175–182. Elsevier Science Publishing,Google Scholar
  2. Bradford M M 1976 A rapid and sensitive method for the quantification of microgram quantities of protein using the principle of protein dye binding. Anal. Biochem. 72, 248–254.PubMedGoogle Scholar
  3. Burggraaf A J P and Shipton W A 1982 Estimates ofFrankia growth under various pH and temperature regimes. Plant and Soil 69, 135–147.Google Scholar
  4. Davey C B and Wollum A G II 1984 Nitrogen fixation systems in forest plantations.In Nutrition of Plantation Forests. Eds. G D Bowen and E K S Nambiar. pp 361–377. Academic Press, London.Google Scholar
  5. Eckholm E 1980 Firewood Crops. pp 1–9. National Academy of Sciences, Washington, DC.Google Scholar
  6. Hoagland D R and Arnon D I 1950 The Water Culture Method for Growing Plants Without Soil. Calif. Agr. Exp. Stat. Circ. 347, Berkeley, CA.Google Scholar
  7. Lalonde M, Calvert H E and Pine S 1982 Isolation and use ofFrankia strains in actinorhizal formation.In Current Perspectives in Nitrogen Fixation. Eds. A H Gibson and W E Newton, pp 296–299. Australian Academy of Science, Canberra.Google Scholar
  8. Murry M A, Fontaine M S and Torrey J G 1984 Growth kinetics and nitrogenase induction inFrankia sp. HFP ArI3 grown in batch culture. Plant and Soil 78, 61–79.Google Scholar
  9. Myers N 1980 Conversion of Tropical Moist Forests. National Academy of Sciences, Washington, DC.Google Scholar
  10. Rosbrook P A and Bowen G D 1987 The abilities of threeFrankia isolates to nodulate and fix nitrogen with four species ofCasuarina. Physiol. Plant. 70, 373–377.Google Scholar
  11. Stowers M D 1987 Collection, isolation, cultivation and maintenance ofFrankia.In Symbiotic Nitrogen Fixation Technology. Ed. G H Elkan. Marcel Dekker, Inc., New York.Google Scholar
  12. Stowers M D, Kulkarni R K and Steele D B 1986 Intermediary carbon metabolism inFrankia. Arch. Microbiol. 143, 319–324.Google Scholar
  13. Stowers M D and Smith J E 1985 Inoculation and production container-grown red alder seedlings. Plant and Soil 87, 153–160.Google Scholar

Copyright information

© Kluwer Academic Publishers 1989

Authors and Affiliations

  • D. Bernie Steele
    • 1
  • K. Ramirez
    • 2
  • M. D. Stowers
    • 3
  1. 1.University of TennesseeKnoxvilleUSA
  2. 2.NPI, Salt Lake CityUSA
  3. 3.Eastman Kodak BioProductsRochesterUSA

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