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Paper electrophoresis of gastric juice in health and disease

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References

  1. Castle, W. B. Observations on etiologic relationship of achylia gastrica to pernicious anaemia. I. Effect of administration to patients with pernicious anaemia of contents of normal human stomach recovered after ingestion of beef muscle.Am. J. M. Sc. 178:748, 1929.

    Google Scholar 

  2. Ternberg, J. L., andEakin, R. E. Erythein and apoerythein and their relation to antipernicious anaemia principle.J. Am. Chem. Soc. 71:3858, 1949.

    Google Scholar 

  3. Hartmann, G. Group Antigens in Human Organs. Copenhagen, Munksgaard, 1941.

    Google Scholar 

  4. Dominici, G., Oliva, G., andTramontana, C. Influence of the gastric juice on tissue metabolism.Lancet 2:1105, 1954.

    Google Scholar 

  5. Feodorov, N. A., Namyatischeva, A. M., andKakhetelidze, M. G. A study of the gastric hematopoietic factor by hemoculture methods.Blood 13:926, 1958.

    PubMed  Google Scholar 

  6. Brunschwig, A., von Prohaska, J., Clarke, T. H., andKandel, E. V. A secretory depressant in gastric juice of patients with pernicious anemia.J. Clin. Invest. 18:415, 1939.

    Google Scholar 

  7. Blackburn, C. M., andCode, C. F. Inhibitors of gastric secretion occurring in gastric juice and gastric mucin.Am. J. Physiol. 155:427, 1948.

    Google Scholar 

  8. Smith, W. O., DuVal, M. K., Joel, W., Honska, W. L., andWolf, S. Gastric atrophy in dogs induced by administration of normal human gastric juice.Gastroenterology 39:55, 1960.

    PubMed  Google Scholar 

  9. Buchs, S. Fundamental observations on the existence, extraction and activation of gastric cathepsin.Enzymologia 16:193, 1954.

    Google Scholar 

  10. Merten, R. Die Bedeutung des Magenkathepsins bei Verdauungsstörungen. Basel, Karger, 1952, pp. 1–72.

    Google Scholar 

  11. Tang, J., Wolf, S., Caputto, R., andTrucco, R. E. Crystallization of gastricsin from human gastric juice.Fed. Proc. 18:337, 1959.

    Google Scholar 

  12. Taylor, W. H. Studies on gastric proteolysis. 4. Proteinase activity of gastric juice and gastric-mucosal extracts atpH 6 to 8.Biochem. J. 71:626, 1959.

    PubMed  Google Scholar 

  13. Glass, G. B. J. Gastric mucin and its constituents. Physico-chemical characteristics, cellular origin, and physiological significance.Gastroenterology 23:636, 1953.

    PubMed  Google Scholar 

  14. Glass, G. B. J., andBoyd, L. J. Enzymatic liquefaction and degradation of mucus in the stomach.Gastroenterology 27:670, 1954.

    Google Scholar 

  15. Capraro, V., Cantone, A., andCresseri, A. Über die Anwesenheit einer resorbierbaren, mit “clearing”-Vermögen versehenen Substanz im normalen Magensaft.Naturwissenschaften 43:36, 1956.

    Google Scholar 

  16. Kozawa, I. S., Iwatsuru, R., Kawaguchi, M., Katagami, M., andUmitsu, A. Über die Veränderung des Blutbildes bei Kanichen durch intravenöse Injektion des Magensaftes von Magenkrebs-Kranken.Folia haematol.57:251, 1937.

    Google Scholar 

  17. Nakahara, W., andFukuoka, F. A first study on toxohormone, a characteristic toxic substance produced by cancer tissue.Gann (Tokyo) 40:45, 1949.

    Google Scholar 

  18. Taylor, W. H. A propos d'une préparation a partir de l'estomac humain d'une substance a activité semblable a celle du facteur intrinsèque.Rev. hémat. 10:206, 1955.

    Google Scholar 

  19. Williams, W. L., Ellenbogen, L., andEsposito, R. G. Preparation of highly purified intrinsic factor.Proc. Soc. Exper. Biol. & Med. 87:400, 1954.

    Google Scholar 

  20. Caputto, R., Schultz, P., Karnes, J., andWolf, W. Chromatographic analysis of fasting human gastric content.Fed. Proc. 13:22, 1954.

    Google Scholar 

  21. Richmond, V., Caputto, R., andWolf, S. Fractionation of the nondialyzable, soluble components of gastric contents by chromatography on Amberlite IRC-50.Arch. Biochem. & Biophys. 66:155, 1957.

    Google Scholar 

  22. Grossberg, A. L., Komarov, S. A., andShay, H. Proteins of canine gastric juice. Electrophoretic characterization and separation of fractions.Am. J. Physiol. 165:1, 1951.

    PubMed  Google Scholar 

  23. Pugh, B. L., Glass, G. B. J., andWolf, S. Electrophoretic studies of the mucin fractions from human gastric juice.Proc. Soc. Exper. Biol. & Med. 79:674, 1952.

    Google Scholar 

  24. Mack, M. H., Wolf, S., andStern, K. G. Preliminary electrophoretic analysis of human gastric juice.J. Clin. Invest. 32:862, 1953.

    PubMed  Google Scholar 

  25. Gräsbeck, R. Studies on the vitamin B12-binding principle and other biocolloids of human gastric juice.Acta med. scandinav., Suppl. 314, 1956.

    Google Scholar 

  26. Katzka, I. The protein distribution of gastric juice electrophoresis; a distinctive pattern in pernicious anemia and achlorhydria.Gastroenterology 36:593, 1959.

    PubMed  Google Scholar 

  27. Pert, J. H. Personal communication.

  28. Jeffries, G., Hoskins, D. W., andSleisenger, M. H. Determination of the vitamin B12 binding capacity of gastric juice by starch gel electrophoresis.Clin. Research 9:153, 1961.

    Google Scholar 

  29. Glass, G. B. J., Stephanson, L., Rich, M., andLaughton, R. W. Intrinsic factor activity of human gastric juice after fractionation by continuous electrophoresis on paper curtain.Brit. J. Haemat. 3:401, 1957.

    PubMed  Google Scholar 

  30. Wada, T., Ohara, H., andEndo, K. Studies on gastric juice protein. III. Polarographical, electrophoretical and biochemical studies on fractionated gastric juice by continuous paper electrophoresis, with special reference to the functional precancerous stage of gastric juice.Gann (Tokyo) 49:249, 1958.

    Google Scholar 

  31. Norpoth, I., Clösges, J., Finger, M., andSchulze, M. Deutsche. med. Wchnschr. 77:561, 1952.

    Google Scholar 

  32. Henning, N., andKinzlmeier, H. Deutsche. med. Wchnschr. 77:998, 1952.

    Google Scholar 

  33. Henning, H., Kinzlmeier, H., andDemling, L. Über die elektrophoretisch darstellbaren Proteine normaler und pathologischer Magensäfte.Münchener med. Wchnschr. 95:423, 1953.

    Google Scholar 

  34. Latner, A. L., Ungley, C. C., Cox, E. V., McEvoy-Bowe, E., andRaine, L. Electrophoresis of human gastric juice in relation to Castle's intrinsic factor.Brit. M. J. 1:467, 1953.

    PubMed  Google Scholar 

  35. Schilling, R. F., andDeiss, W. P. Intrinsic factor studies. I. Paper electrophoresis of mixture of gastric juice and radioactive vitamin B12.Proc. Soc. Exper. Biol. & Med. 83:506, 1953.

    Google Scholar 

  36. Wada, T., Ohara, H., andYoshikawa, H. Studies on gastric juice protein. II. Electrophoretical studies on fractionated gastric juice, pertaining to histamine refractory anacidic specimen.Gann (Tokyo) 49:261, 1958.

    Google Scholar 

  37. Jarnum, S., andSchwartz, M. Hypoalbuminemia in gastric carcinoma.Gastroenterology 38:769, 1960.

    PubMed  Google Scholar 

  38. Hollander, F., andHorowitz, M. I. Serum albumin (Alb) in canine gastric acetylcholine-mucus (ACh-Mu) and whole stomach aspirate.Fed. Proc. 19:181, 1960.

    Google Scholar 

  39. Glass, G. B. J., Stephanson, L., andRich, M. Paper-electrophoretic analysis of gastric juice.Fed. Proc. 15:75, 1956.

    Google Scholar 

  40. Glass, G. B. J., Stephanson, L., andRich, M. Paper-electrophoretic analysis of normal human gastric juice under basal conditions and following humoral and vagal stimulation. Abstract of communication to XX International Physiology Congress, Brussels, July 30–Aug. 4, 1956, pp. 346–347.

  41. Glass, G. B. J., Stephanson, L., andRich, M. Paper-electrophoretic analysis of gastric juice in health and disease and its physiological and clinical significance.Gastroenterologia 86:384, 1956.

    PubMed  Google Scholar 

  42. Glass, G. B. J., Rich, M., andStephanson, L. Comparative study of mucoproteins of human gastric juice and serum.Gastroenterology 34:598, 1958.

    PubMed  Google Scholar 

  43. Glass, G. B. J., Stephanson-Liounis, L., Rich, M., andMitchell, S. E. Quantitation of pepsin in gastric juice by paper-electrophoresis.Fed. Proc. 17:55, 1958.

    Google Scholar 

  44. Glass, G. B. J., Stephanson-Liounis, L., Rich, M., andMitchell, S. E. “Paper electrophoretic analysis of gastric juice in gastric anacidity, gastric atrophy and cancer of the stomach,” inProceedings of the World Congress of Gastroenterology. Baltimore, Williams & Wilkins, 1958, pp. 994, 1002.

    Google Scholar 

  45. Glass, G. B. J., andUchino, H. Vitamin B12 binders in human gastric juice.Fed. Proc. 18:53, 1959.

    Google Scholar 

  46. Glass, G. B. J., Uchino, H., andSchwartz, G. Study of vitamin B12 binders in gastric juice of normals, pernicious anemia patients and individuals with atrophic gastritis and histamine-fast anacidity. Abstract, pp. 262–264, Hemopoietic Factors, 8th International Congress of Hematology, Sept. 4–10, 1960, Tokyo, Japan.

  47. Glass, G. B. J., andIshimori, A. Paper electrophoretic detection of leakage of albumin into the gastric juice in protein-losing gastropathies and gastric cancer.Fed. Proc. 19:191, 1960.

    Google Scholar 

  48. Glass, G. B. J., andIshimori, A. Passage of serum proteins into the stomach under normal conditions and in protein-losing gastropathies, including gastric cancer; its detection by paper electrophoresis of gastric juice.Am. J. Digest. Dis. 6:103, 1961.

    PubMed  Google Scholar 

  49. Ishimori, A., andGlass, G. B. J. Paper electrophoresis of native gastric juice after its concentration by dialysis against Carbowax 6000.Clin. Chemistry 7:457, 1961.

    Google Scholar 

  50. Hitzelberger, A. L., andGlass, G. B. J. Effect of corticosteroids on non-dialyzable substances of gastric juice.Fed. Proc. 19:190, 1960.

    Google Scholar 

  51. Hitzelberger, A. L., andGlass, G. B. J. Effect of corticosteroids in humans on the secretion of large molecular substances of gastric juice.J. Lab. & Clin. Med., submitted for publication.

  52. Ohara, H., andGlass, G. B. J. In preparation.

  53. Williams, F. G., Jr., Pickets, E. G., andDurrum, E. L. Improved hanging strip paper electrophoresis technique.Science 121:928, 1955.

    Google Scholar 

  54. Rideout, L. A., andPrichard, R. W. Inexpensive stain for paper electrophoresis.Science 121:374, 1955.

    PubMed  Google Scholar 

  55. Köiw, E., andGrönwall, A. Staining of protein-bound carbohydrates after electrophoresis of serum on filter paper.Scandinav. J. Clin. & Lab. Invest. 4:244, 1952.

    Google Scholar 

  56. Northcote, D. H. Electrophoresis of some neutral polysaccharides.Biochem. J. 58:353, 1954.

    PubMed  Google Scholar 

  57. Smithies, O. Zone electrophoresis in starch gels; group variations in the serum proteins of normal human adults.Biochem. J. 61:629, 1955.

    PubMed  Google Scholar 

  58. Pert, J. H., Engle, R. E., Jr., Woods, K. R., andSleisenger, M. H. Preliminary studies on quantitative zone electrophoresis in starch gel.J. Lab. & Clin. Med. 54:572, 1959.

    Google Scholar 

  59. Verschure, J. C. M. “Comparative studies of the protein fractions from human gastrointestinal juices with paper electrophoresis combined with various detection methods,” inProtides of the Biologic Fluids, Edited by H. Peeters. Amsterdam, Elsevier, 1959, pp. 194–201.

    Google Scholar 

  60. Jacob, T. A., Williams, D. H., Howe, E. E., andGlass, G. B. J. Ultracentrifugation and paper-electrophoresis of highly active intrinsic factor preparations.Arch. Biochem. & Biophys. 81:522, 1959.

    Google Scholar 

  61. Glass, G. B. J., Jacob, T. A., Williams, D. E., andHowe, E. E. Correlation of intrinsic factor activity of hog stomach preparations with their paper-electrophoretic patterns, sedimentation constants and B12-binding capacity.Tohoku J. Exper. Med. 71:1, 1959.

    Google Scholar 

  62. Glass, G. B. J. Localization of intrinsic factor and the sites of B12-binding in the paper electrophoresis of human gastric juices and concentrates from hog gastric mucosa.Haematol. Latina 2:231, 1959.

    Google Scholar 

  63. Glass, G. B. J., andSkoryna, S. C. Paper-electrophoretic analysis of the secretion from the explanted gastric mucosa in rats.Clin. Res. Proc. 6:270, 1958.

    Google Scholar 

  64. Glass, G. B. J., andSkoryna, S. C. Paper electrophoretic study of the proteinlosing secretions from explanted gastric mucosa in rats.Proc. Soc. Exper. Biol. & Med. 107:560, 1961.

    Google Scholar 

  65. Norpoth, L., Surmann, T., andClösges, J. Über den Ort der Magenfermente im Elektrophoresediagramm.Klin. Wchnschr. 31:1005, 1953.

    Google Scholar 

  66. Wunderly, C. Control of the staining procedure after paper electrophoresis. Comparison of the spreading on paper of protein fractions.Acta. haemat. 18:272, 1957.

    PubMed  Google Scholar 

  67. Kinersly, T. Preliminary paper electrophoretic study of saliva.Yale J. Biol. & Med. 26:211, 1953.

    Google Scholar 

  68. Sasai, T., Kakei, M., Shinohara, H., Kubo, K., andIsogawa, K. Studies on the gastric juice protein. On the “peptide portion” of the gastric juice by the paper-electrophoresis polarogram.Bull. Inst. Chem. Res. Kyoto Univ. 37:15, 1959.

    Google Scholar 

  69. Kakei, M. Studies on the gastric juice protein. II. Paper electrophoresis-polarographic study of gastric juice protein.Arch. Japan. Chirurgie 28:2621, 1959.

    Google Scholar 

  70. Gullberg, R., andOlhagen, B. Electrophoresis of human gastric juice.Nature, London 184:1848, 1959.

    Google Scholar 

  71. Glass, G. B. J., Ishimori, A., Hitzelberger, A. L., Rich, M., andStephanson-Liounis, L. Study of the proteolytic enzymes of gastric juice.Clin. Res. 8:202, 1960.

    Google Scholar 

  72. Holman, H., Nickel, W. F., andSleisenger, M. H. Hypoproteinemia antedating intestinal lesions, and possibly due to excessive serum protein loss into the intestine.Am. J. Med. 27:963, 1959.

    PubMed  Google Scholar 

  73. Werner, I. Studies on glycoproteins from mucous epithelium and epithelial secretion.Acta Soc. med. upsal. 58:1, 1953.

    Google Scholar 

  74. Blix, G. Mucopolysaccharides and glycoproteins.Scandinav. J. Clin. & Lab. Invest. 10:Suppl. 31, 128, 1957.

    Google Scholar 

  75. Glass, G. B. J., andBoyd, L. J. Studies on dissolved mucin of the gastric juice. IV. Relationship of the mucoid of the visible gastric mucus, its split products, and the salivary mucin to the dissolved gastric mucoproteose and mucoprotein of the gastric juice.Bull. New York Med. Coll. 11:8, 1948.

    Google Scholar 

  76. Glass, G. B. J., andBoyd, L. J. The three main components of the human gastric mucin: Dissolved mucoproteose, dissolved mucoprotein, and mucoid of the gastric visible mucus. I. Differentiation; some physical and chemical characteristics; classification. II. Method for separation and quantitative determination of each mucous component of the gastric juice. III. Preliminary data on physiological and clinical significance of separate quantitative determination of the dissolved mucoproteose and dissolved mucoprotein in the gastric juice of man.Gastroenterology 12:821, 1949.

    Google Scholar 

  77. Glass, G. B. J., Pugh, B. L., Grace, W. J., andWolf, S. Observations on the treatment of human gastric and colonic mucus with lysozyme.J. Clin. Invest. 29:12, 1950.

    PubMed  Google Scholar 

  78. Janowitz, H. D., andHollander, F. Viscosity of cell-free canine gastric mucus.Gastroenterology 26:582, 1954.

    PubMed  Google Scholar 

  79. Glass, G. B. J. Large molecule substances of gastric juice: their fractionation by electrophoresis and other physico-chemical technics.Advances Clin. Chem. vol. 4, in press.

  80. Babkin, B. P. Secretory Mechanisms of the Digestive Glands. New York, Hoeber, 1950.

    Google Scholar 

  81. Glass, G. B. J., Speer, F. D., Nieburgs, H. E., Ishimori, A., Jones, E. L., Baker, H., Schwartz, S. A., andSmith, R. Gastric atrophy, atrophic gastritis and gastric secretory failure: correlative study by suction biopsy and exfoliative cytology of gastric mucosa, paper electrophoretic and secretory assays of gastric secretion, and measurements of intestinal absorption and blood levels of vitamin B12.Gastroenterology 39:429, 1960.

    PubMed  Google Scholar 

  82. Citrin, Y., Sterling, K., andHalsted, J. A. The mechanism of hypoproteinemia associated with giant hypertrophy of gastric mucosa.New England J. Med. 257: 906, 1957.

    Google Scholar 

  83. Solomon, E. Deutsche. med. Wchnschr. 1903, p. 546. (Cited in reference 33.)

  84. Wolff, W., andJunghans, P. Berl. Klin. Wchnschr., 1911, p. 978. (Cited ibidem.)

  85. Katsch, G. Klin. Wchnschr., 1935, p. 1561. (Cited ibidem.)

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I wish to acknowledge the courtesy of Dr. F. Kern. Jr., of the V. A. Hospital, Denver, Colo., Dr. H. Janowitz of the Mount Sinai Hospital, New York, N. Y.. Dr. H. Colcher of the Goldwater Memorial Hospital (Columbia Division), New York, N. Y.. and Dr. I. Arias of the Albert Einstein College of Medicine, New York, N. Y., in providing specimens of gastric juice from patients with protein-losing gastropathies. and of Dr. Cyrus Rubin, who supplied gastric juices of patients with “juvenile pernicious anemia.”

Supported by Research Grant in Aid A-68 (C3–9) from the Institute of Arthritis and Metabolic Disease. National Institutes of Health. U.S.P.H.S. and the American Cancer Society.

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Glass, G.B.J. Paper electrophoresis of gastric juice in health and disease. Digest Dis Sci 6, 1131–1192 (1961). https://doi.org/10.1007/BF02231812

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