The American Journal of Digestive Diseases

, Volume 17, Issue 7, pp 603–611 | Cite as

Influence of cholinergic stimulation on gastric secretory response to histamine in ex vivo isolated canine stomach

  • K. Kowalewski
  • R. Scharf


Totally isolated canine stomach, included into a perfusion system containing a living “supporting” dog, serving as an oxygenator, is able to secrete juice rich in H+ for several hours. Blood circulating in this system is homologous blood and the stomach may be stimulated by intraarterial continuous infusion of a secretion stimulant. The ex vivo preparation was used for the study of secretion stimulated by histamine and/or bethanechol chloride, a stable cholinergic drug. All combinations of histamine plus bethanechol chloride used in this study gave higher outputs of HCl and pepsin than could be accounted for on the basis of summation of stimulatory effects. Outputs of HCl and pepsin were higher when two agents were given together than the maximal output of either HCl or pepsin during stimulation by one agent. Potentiation with regard to acid and pepsin secretion was evident when a cholinergic drug, in various doses, was superimposed on histamine stimulation.


Public Health Chloride Histamine Continuous Infusion Maximal Output 
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  1. 1.
    Fingl E, Woodbury DM: General principles, The Pharmacological Basis of Therapeutics. Edited by LS Goodman, A Gilman. MacMillan Company, 1970, p 24Google Scholar
  2. 2.
    Cooke AR: Potentiation of acid secretion in dogs. Am J Physiol 216:968, 1969PubMedGoogle Scholar
  3. 3.
    Uvnas B: The part played by the pyloric region in the cephalic phase of gastric secretion. Acta Physiol Scand [Suppl] 13:1, 1942Google Scholar
  4. 4.
    Grossman MI: Integration of neural and hormonal control of gastric secretion. Physiologist 6:349, 1963Google Scholar
  5. 5.
    Gillespie IE, Grossman MI: Potentiation between urecholine and gastrin extract and between urecholine and histamine in the stimulations of Heidenhain pouches. Gut 5:71, 1964Google Scholar
  6. 6.
    Marks IN, Komarov SA, Shay H: Influence of cholinergic stimuli on gastric secretory responses to histamine in the dog. Am J Dig Dis 11:122, 1966PubMedGoogle Scholar
  7. 7.
    Passaro EP, Gillespie IE, Grossman MI: Potentiation between gastrin and histamine in stimulation of gastric secretion. Proc Soc Exp Biol Med 114:50, 1963PubMedGoogle Scholar
  8. 8.
    Dritsas KG, Kowalewski K: Perfusion of the isolated canine stomach. Br J Surg 53:732, 1966PubMedGoogle Scholar
  9. 9.
    Dritsas KG, Bondar GF, Kowalewski K: Secretory response to sustained histamine stimulation of the isolated canine stomach. Br J Surg 53:798, 1966PubMedGoogle Scholar
  10. 10.
    Dritsas KG, Kowalewski K: Further studies on ex vivo isolated perfused canine stomach: Role of antrum as an inhibitor of gastric secretory function. Scand J Gastroenterol 3:99, 1968PubMedGoogle Scholar
  11. 11.
    Dritsas KG, Kowalewski K: The mechanism of pepsin secretion in the isolated perfused canine stomach. Can J Surg 11:85, 1968PubMedGoogle Scholar
  12. 12.
    Fisk RL, Brownlee RT, Brown DR, et al: Perfusion of isolated organs for prolonged functional preservation, Organ Perfusion and Preservation. Edited by JC Norman. Appleton-Century Crofts, New York, 1968, p 217Google Scholar
  13. 13.
    Fisk RL, Kowalewski K: Study of gastrone-like activity of intestinal secretion in the isolated perfused canine stomach. Can J Surg 13:72, 1970PubMedGoogle Scholar
  14. 14.
    Kowalewski K, Fish RL, Schier JF: Perfusion of the isolated canine stomach—evaluation of the usefulness of a new method for the study of gastric secretion. Q J Surg Sci 6:1, 1970Google Scholar
  15. 15.
    Fisk RL, McFarlane DF, Kowalewski K, Couves CM: Electronic feedback blood pump controls for isolated organ perfusion. J Appl Physiol 27:403, 1969PubMedGoogle Scholar
  16. 16.
    Anson MI: The estimation of pepsin, trypsin, papain and cathepsin with hemoglobin. J Gen Physiol 22:79, 1938CrossRefGoogle Scholar
  17. 17.
    Aitken MA, Spray GH, Walters G: Gastric pepsin and the excretion of uropepsinogen in anemia. Clin Sci 13:119, 1954PubMedGoogle Scholar
  18. 18.
    Stavraky GW: Some aspects of gastric secretion induced by Mecholyl. Rev Can Biol 2:59, 1943Google Scholar
  19. 19.
    Pevsner L, Grossman MI: The mechanism of vagal stimulation of gastric acid secretion. Gastroenterology 28:493, 1955PubMedGoogle Scholar

Copyright information

© Harper & Row, Publishers 1972

Authors and Affiliations

  • K. Kowalewski
    • 1
  • R. Scharf
    • 1
  1. 1.From the Surgical-Medical Research Institute and Department of SurgeryThe University of AlbertaEdmontonCanada

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