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Clinical Rheumatology

, Volume 15, Issue 1, pp 31–34 | Cite as

Human gelatinase B, a marker enzyme in rheumatoid arthritis, is inhibited by D-penicillamine: Anti-rheumatic activity by protease inhibition

  • K. Norga
  • B. Grillet
  • S. Masure
  • L. Paemen
  • G. Opdenakker
Originals

Summary

The direct and indirect inhibitory potential of D-penicillamine toward human neutrophil and synovial fluid gelatinase B, a marker enzyme for disease severity in RA, was investigated. Gelatinase and plasminogen activator activities were assessed by SDS-polyacrylamide gel electrophoresis zymography. D-penicillamine significantly inhibits purified and synovial fluid gelatinase Bin vitro at concentrations attainablein vivo and also blocksin vitro plasminogen activation. Protease inhibition may be a mechanism of action for D-penicillamine as DMARD.

Key words

Gelatinase Matrix Metalloproteinases Penicillamine Protease Inhibitor Plasminogen Activator Rheumatoid Arthritis 

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Copyright information

© Clinical Rheumatology 1996

Authors and Affiliations

  • K. Norga
    • 1
    • 2
  • B. Grillet
    • 2
  • S. Masure
    • 1
    • 2
  • L. Paemen
    • 1
    • 2
  • G. Opdenakker
    • 1
    • 2
  1. 1.From the Rega Institute for Medical ResearchLeuvenBelgium
  2. 2.the Division of Rheumatology, Faculty of MedicineUniversity of LeuvenBelgium

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