Journal of Neuro-Oncology

, Volume 3, Issue 2, pp 181–186 | Cite as

Immunolocalization of monoclonal antibody-defined extracellular matrix antigens in human brain tumors

  • Rodney D. McComb
  • Darell D. Bigner


The extracellular matrix is involved in many aspects of tumor cell biology, including tumor invasion and metastasis. 2A6 and 81C6 are murine monoclonal antibodies that identify glioma-mesenchymal extracellular matrix antigens. The 81C6 antigen is a high molecular weight glycoprotein composed of Mr 230,000 subunits. The expression of 2A6 antigen, 81C6 glycoprotein, fibronectin (FN), and laminin (LN) was examined immunohistochemically in ten malignant gliomas (MG) and four medulloblastomas (MBT). 2A6 and 81C6 were expressed in similar patterns by the neoplastic neuroepithelial cells in 9/10 MG and 1/4 MBT. The staining was typically diffuse and amorphous, without visualization of distinct cell bodies or processes. Less frequently, antigen was detected within tumor cell cytoplasm. In most tumors the staining was greatest in the perivascular regions. In two MG, 2A6 and 81 C6 were expressed only by a subpopulation of neoplastic cells. Although intense staining was also associated with hyperplastic vascular and mesenchymal cells, many small and medium size blood vessels stained weakly or not at all. In contrast, FN and LN were expressed uniformly and intensely in the tumor vasculature, but were not expressed by neoplastic neuroepithelial cells. The 2A6 antigen and 81C6 glycoprotein are immunohistochemically distinct from FN and LN. These monoclonal antibody-defined antigens are heterogeneously expressed by neoplastic neuroepithelial cells and hyperplastic vascular-mesenchymal elements in MG and MBT. The 2A6 and 81C6 monoclonal antibodies will be useful reagents in the investigation of the extracellular matrix of malignant neuroepithelial neoplasms.

Key words

extracellular matrix monoclonal antibody fibronectin laminin brain neoplasm invasion 


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Copyright information

© Martinus Nijhoff Publishers 1985

Authors and Affiliations

  • Rodney D. McComb
    • 1
  • Darell D. Bigner
    • 2
  1. 1.Department of Pathology and Laboratory MedicineUniversity of Nebraska Medical CenterOmaha
  2. 2.Department of PathologyDuke University Medical CenterDurham

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