Labelling of mouse spermatozoa with L-arginine-14C(U) monohydrochloride
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Two mouse males were injected with L-arginine-14C(U) monohydrochloride, one with a dose of 100 μ Ci, the other with a dose of 500 μ Ci. Mating of both males was accomplished at regular intervals. Sperm was collected from uteri, smeared and coated for grain density autoradiography. Autoradiography revealed a sharp peak of the proportion of labelled spermatozoa in the ejaculate on the 13th day after injection followed by a rapid decrease on the 16th and 20th day. The most heavily labelled spermatozoa from the peak and ova fertilized by them were further investigated by means of track autoradiography. Spermatozoa in the ejaculate smear and spermatozoa and male pronuclei in histologic sections from the ampullar part of the oviduct were checked for the presence of tracks. The number of tracks originating from spermatozoa in the vicinity of the fertilized ova was about the same as that observed in freshly ejaculated spermatozoa. By contrast, no tracks were detected in male pronuclei. This observation is interpreted as an evidence of a possible loss of the argininerich histone of the sperm at the time of pronuclear formation.
Key-WordsSpermatozoa Labelling Arginine-14C
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