Abstract
In order to evaluate the suitability ofAzospirillum spp. as a crop inoculant in temperate regions, the natural occurrence, distribution and survival ofAzospirillum after seed inoculation in Belgian agricultural soils was studied.Azospirillum was present in most of the fields examined, but concentrations never exceeded 1000 cfu per g soil or per g roots. Under field conditions none of the known species was found to be localized inside the roots of barley, wheat, rye, maize or grasses. Also, the distribution ofA. brasilense SpBr 14 within the root system of hydroponic-grown wheat was studied by immunofluorescence. From the rhizosphere samples of the field crops investigated, a number of microaërophilic, diazotrophic bacteria were isolated and identified asA. lipoferum, found only on maize and grass roots, andA. brasilense, present under all crops. In contrast toA. brasilense, A. lipoferum was able to use different amino-acids and some derivatives as sole carbon and nitrogen sources. Use of a peat-based seed inoculant resulted in the establishment of theAzospirillum spp. in the rhizosphere of field-grown winter barley and winter wheat. The established population survived during winter without appreciable change in numbers, but there was no indication of active growth during spring or summer.
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Bashan Y and Levanony H 1987 Interaction betweenAzospirillum brasilense Cd and wheat root cells during early stages of root colonization.In Azospirillum 4, Genetics, Physiology, Ecology. Ed. W Klingmüller, Springer Verlag.
Horemans S, De Coninck K, Neuray J, Hermans R and Vlassak K 1986 Production of plant growth substances byAzospirillum spp. and other rhizosphere bacteria. Symbiosis 2, 341–346.
Kersters K, Hinz K H, Hertle A, Segers P, Lievens A, Siegman O and Deley J 1984Bordetella avium sp. nov., isolated from the respiratory tracts of turkeys and other birds. Int. J. Syst. Bacteriol. 34, 57–70.
Markus P and Kramer J 1987 Importance of non-symbiotic nitrogen-fixing bacteria in organic farming systems.In Azospirillum 4, Genetics, Physiology, Ecology Ed. W Klingmüller, Springer Verlag.
Patriquin D G and Döbereiner J 1978 Light microscopy observations of tetrazolium-reducing bacteria in the endorhizosphere of maize and other grasses in Brazil. Can. J. Microbiol. 24, 734–742.
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De Coninck, K., Horemans, S., Randombage, S. et al. Occurrence and survival ofAzospirillum spp. in temperate regions. Plant Soil 110, 213–218 (1988). https://doi.org/10.1007/BF02226801
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DOI: https://doi.org/10.1007/BF02226801