Cell cycle related changes in the quantity of TMV virions bound to protoplasts ofNicotiana sylvestris
Attachment of virions of tobacco mosaic virus to protoplasts isolated from dividing suspension cultured cells ofNicotiana sylvestris was estimated using quantitative autoradiography of individual protoplasts. Additionally, the position of each protoplast in the cell cycle was assessed by Feulgen microspectrophotometry. At pH 5.6, after preincubation with 4 μg 1−1 poly-L-ornithine, protoplasts in the G1 and G2 phases bound more virions than protoplasts in the S-phase. The possibility that such differential binding was caused by cyclical variation in the net charge on the protoplast membrane has been investigated. It was found that S-phase protoplasts ofN. sylvestris can be separarated from protoplasts of other cycle stages by partition in aqueous, two-phase, immiscible polymer systems, presumably because they differ in charge. Also, electrophoretic studies suggest that G1 phase protoplasts bear higher surface charge than some non-G1 protoplasts.
KeywordsCell cycle Membrane charge Nicotiana Plant protoplasts Tobacco mosaic virus
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