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Involvement of protein kinase C in the histamine H1-receptor mediated contraction of guinea-pig lung parenchymal strips

  • Histamine Receptors and Antihistamines
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Abstract

In this study we investigated the role of protein kinase C (PKC) in the histamine H1-receptor mediated contraction of guinea-pig parenchymal lung strips. Lung strips contract after administration of the PKC activators phorbol-12-myristate-13-acetate (PMA) and dioctanoylglycerol. Prolonged stimulation (45 min.) with a high concentration of PMA abolished subsequent responses to PMA whereas H1-responses were only partially reduced. Administration of a PKC inhibitor (H-7) also led to an inhibition of H1-responses. Experiments with combined incubations with the PKC inhibitor H-7 and the Ca2+-entry blocker verapamil suggest that the PKC contribution to the response is possibly mediated via regulation of Ca2+ influx.

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Authors and Affiliations

  1. Department of Pharmacochemistry, Faculty of Chemistry, Vrije Universiteit, De Boelelaan 1083, 1081 HV, Amsterdam, The Netherlands

    R. Leurs, J. N. L. Go, A. Bast & H. Timmerman

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  1. R. Leurs
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  2. J. N. L. Go
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  3. A. Bast
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  4. H. Timmerman
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Leurs, R., Go, J.N.L., Bast, A. et al. Involvement of protein kinase C in the histamine H1-receptor mediated contraction of guinea-pig lung parenchymal strips. Agents and Actions 27, 180–183 (1989). https://doi.org/10.1007/BF02222233

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  • DOI: https://doi.org/10.1007/BF02222233

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