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, Volume 27, Issue 1–2, pp 52–54 | Cite as

Enhancement of the histamine releasing activity of mouse monoclonal anti-human IgE antibody xb6-16 when present in aggregated or complexed forms

  • A. Weyer
  • J. P. Dandeu
  • M. Etiévant
  • J. P. Bourgeois
  • F. Marchand
  • A. Prouvost-Danon
  • B. David
Histamine Release from Murine Serosal Mast Cells and Human Basophils

Abstract

FPLC purification of mouse monoclonal anti-human IgE antibody xb6-16 showed 2 major peaks of different molecular weight, peak 1 (>106 d) and peak 3 (1.6×105 d). Peak 1 consisted of IgG 1 and IgM, peak 3 of IgG 1 only. On a protein weight basis, peak 1 was 100 times more potent than peak 3 in inducing histamine release from human basophils. Preincubation of peak 3 with anti-IgG 1 enhanced the mediator release triggered by this fraction. On this basis, the potentiating effect of aggregated IgG 1 or IgG 1-IgM complexes on mediator release from basophils is discussed.

Keywords

Molecular Weight Histamine Weight Basis Major Peak Histamine Release 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Birkhäuser Verlag 1989

Authors and Affiliations

  • A. Weyer
    • 1
  • J. P. Dandeu
    • 1
  • M. Etiévant
    • 1
  • J. P. Bourgeois
    • 2
  • F. Marchand
    • 1
  • A. Prouvost-Danon
    • 1
  • B. David
    • 1
  1. 1.Unité d'Immuno-AllergieInstitut PasteurParis
  2. 2.Diagnostics PasteurMarnes-la-CoquetteFrance

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