Enhancement of the histamine releasing activity of mouse monoclonal anti-human IgE antibody xb6-16 when present in aggregated or complexed forms
FPLC purification of mouse monoclonal anti-human IgE antibody xb6-16 showed 2 major peaks of different molecular weight, peak 1 (>106 d) and peak 3 (1.6×105 d). Peak 1 consisted of IgG 1 and IgM, peak 3 of IgG 1 only. On a protein weight basis, peak 1 was 100 times more potent than peak 3 in inducing histamine release from human basophils. Preincubation of peak 3 with anti-IgG 1 enhanced the mediator release triggered by this fraction. On this basis, the potentiating effect of aggregated IgG 1 or IgG 1-IgM complexes on mediator release from basophils is discussed.
KeywordsMolecular Weight Histamine Weight Basis Major Peak Histamine Release
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