Current Genetics

, Volume 29, Issue 2, pp 122–129 | Cite as

The cloning and sequencing of thealcB gene, coding for alcohol dehydrogenase II, inAspergillus nidulans

  • Gary D. Hunter
  • I. Gwyn Jones
  • Heather M. Sealy-Lewis
Original Paper


Alcohol dehydrogenase II (ADH II, structural genealcB) was purified from a strain H1035,biA1; alcE1; alc500 alcD1, which produces 100-times more ADH II activity than thealcAalcR deletion strain (alc500). Antibodies were raised against this ADH, and were used to screen a cDNA library in γgt11. We have isolated the gene for an ADH which is over-expressed in H1035, and which we believe to be thealcB gene; cDNA and genomic clones were sequenced. The sequence contains three introns and encodes a protein of 367 amino acids. This protein shows a clear level of identity to a range of alcohol dehydrogenases, but is no more closely related to the ADH I and ADH III previously described inA. nidulans than to the ADHs ofS. pombe andS. cerevisiae. The significance of consensus sequences found in the 5′ region of the gene is discussed in relation to the regulation of the gene.

Key words

Alcohol dehydrogenase II Aspergillus nidulans 


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Copyright information

© Springer-Verlag 1996

Authors and Affiliations

  • Gary D. Hunter
    • 1
  • I. Gwyn Jones
    • 1
  • Heather M. Sealy-Lewis
    • 1
  1. 1.Department of Applied BiologyUniversity of HullHullUK

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