Identification of genes expressed in the shoot apex ofBrassica campestris during floral transition
- 23 Downloads
The vegetative-to-floral transition ofBrassica campestris cv. Osome was induced by vernalization. Poly(A)+RNA was isolated from the transition shoot apex after 6 weeks of vernalization, the floral apex after 12 weeks of vernalization and the expanded leaves just before vernalization, and cDNAs were synthesized. These cDNAs were used for subtraction and differential screening to select cDNA preferentially present in the transition and floral apices. Nucleotide sequences of the resulting 14 cDNA clones were determined, and northern blot analysis was carried out on six cDNAs. Two cDNA clones which did not show significant similarity to known genes were shown to be preferentially expressed in the floral apex.
Key wordsBrassica campestris cDNA cloning Floral transition Shoot apex Vernalization
Unable to display preview. Download preview PDF.
- Kawashima I, Inokuchi Y, Chino M, Kimura M, Shimizu N (1991) Isolation of a gene for a metallothionein-like protein from soybean. Plant Cell Physiol 32:913–916Google Scholar
- Newman T, Bruijn FJ de, Green P, Keegstra K, Kende H, McIntosh L, Ohlrogge J, Raikhel N, Somerville S, Thomashow M, Retzel E, Somerville C (1994) Genes galore: a summary of methods for accessing results from large-scale partial sequencing of anonymousArabidopsis cDNA clones. Plant Physiol 106:1241–1255CrossRefPubMedGoogle Scholar
- Stafstrom JP, Sussex IM (1992) Expression of a ribosomal protein gene in axillary buds of pea seedlings. Plant Physiol 100:1494–1502Google Scholar
- Yui S, Yoshikawa H (1992) Breeding of bolting resistance in Chinese cabbage — critical day length for flower induction of latebolting material with no chilling requirement. J Jpn Soc Hortic Sci 61:565–568Google Scholar