Skip to main content
SpringerLink
Log in

Inhibitory effect of cadmium on calcium absorption from the rat duodenum

  • Published:
Archives of Environmental Contamination and Toxicology Aims and scope Submit manuscript

Abstract

Male rats of the Wistar strain (6 weeks old) were divided into 2 groups; control group and experimental group. The control group was fed a cadmium-free diet. The experimental group was fed a diet containing 100 ppm of cadmium (CdCl2). During the 33-day period of cadmium intake, body weight was recorded. For the 7th to 10th, 16th to 19th, and 25th to 28th day of the experimental period, food consumption was recorded and at the same time urine and feces were sampled to obtain the calcium and phosphorus concentrations. On the 8th, 17th, and 26th day, about 0.2 ml of blood was sampled to determine the calcium and phosphorus concentration. The concentration of calcium remained stationary during the experimental period, but the concentration of phosphorus in the experimental group was significantly lower than that in the control group. On the 29th or 30th day45Ca(1ΜCi) was given orally with the cold calcium, and the 1- and 2-hr blood was collected by heart puncture to obtain the45Ca activity in the serum. The 1-hr activity in the control group was significantly higher than that in the experimental group. On the final day, the rats were sacrificed to obtain the duodenum and kidney calcium-binding protein. The binding activities of these proteins in the experimental group were significantly lower than that in the control group.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Allen, R. J. L.: The estimation of phosphorus. Biochem. J.34, 858 (1940).

    Google Scholar 

  • Doyle, J. J., W. H. Pfander, S. E. Graebing, and J. O. Pierce: Effect of dietary cadmium on growth, cadmium absorption and cadmium tissue of dietary cadmium on growth, cadmium absorption and cadmium tissue levels in growing lambs. J. Nutr.104, 160 (1974).

    PubMed  Google Scholar 

  • Drescher, D., and H. F. DeLuca: Vitamin D stimulated calcium binding protein from rat intestinal mucosa—purification and some properties. Biochemistry10, 2302 (1971).

    Article  PubMed  Google Scholar 

  • Harmeryer, J., and H. F. DeLuca: Calcium-binding protein and calcium absorption after vitamin D administration. Arch. Biochem. Biophys.133, 247 (1969).

    Article  PubMed  Google Scholar 

  • Ingersoll, R. J., and R. H. Wasserman: Vitamin D3-induced calcium-binding protein. J. Biol. Chem.246, 2808 (1971).

    PubMed  Google Scholar 

  • Kazantzis, G., F. V. Flynn, J. S. Spowage, and D. G. Trott: Renal tubular malfunction and pulmonary emphysema in cadmium pigment workers. Quant. J. Med.32, 165 (1963).

    Google Scholar 

  • Kimura, M., N. Otaki, S. Yoshiki, M. Suzuki, and T. Suda: The role of metallothionein in cadmium poisoning. Jap. J. Hyg.29, 66 (1974).

    Google Scholar 

  • Lowry, O. H., N. J. Rosebrough, A. L. Farr, and R. J. Randall: Protein measurement with folin phenol reagent. J. Biol. Chem.193, 265 (1951).

    PubMed  Google Scholar 

  • Nicaud, P., A. Lafitte, and A. Gros: Les troubles de l'intoxication chronique par le cadmium. Arch. Mal. prof.4, 192 (1942).

    Google Scholar 

  • Nordin, B. E. C., M. M. Young, C. Oxby, and L. Bulusu: Calculation of calcium absorption rate from plasma radioactivity. Clin. Sci.35, 177 (1968).

    PubMed  Google Scholar 

  • Piazolo, P., M. Schleyer, and H. E. Franz: Isolation and purification of a calcium-binding protein from human tissues. Hoppe-Seyler's Z. Physiol. Chem.352, 1480 (1971).

    PubMed  Google Scholar 

  • Powell, G. W., W. J. Miller, J. D. Morton, and C. M. Clifton: Influence of dietary cadmium level and supplemental zinc on cadmium toxicity in the bovine. J. Nutr.84, 205 (1964).

    PubMed  Google Scholar 

  • Sands, H., and R. H. Kessler: A calcium binding component of dog kidney cortex and its relationship to calcium transport. Proc. Soc. Exp. Biol. Med.137, 1267 (1971).

    PubMed  Google Scholar 

  • Sugawara, C., and N. Sugawara: Cadmium toxicity for rat intestine, especially on the absorption of calcium and phosphorus. Jap. J. Hyg.28, 511 (1974a).

    PubMed  Google Scholar 

  • Sugawara, N., and C. Sugawara: Cadmium accumulation in organs and mortality during a continued oral uptake. Arch. Toxicol.32, 297 (1974b).

    Article  PubMed  Google Scholar 

  • Tanaka, Y., and H. F. DeLuca: The control of 25-hydroxy vitamin D metabolism by inorganic phosphorus. Arch. Biochem. Biophys.154, 566 (1973).

    Article  PubMed  Google Scholar 

  • Taylor, A. N., and R. H. Wasserman: Vitamin D-induced calcium-binding protein—comparative aspects in kidney and intestine. Amer. J. Physiol.223, 110 (1972).

    PubMed  Google Scholar 

  • Wasserman, R. H., A. N. Corradino, and A. N. Taylor: Vitamin D-dependent calciumbinding protein—purification and some properties. J. Biol. Chem.243, 3978 (1968).

    PubMed  Google Scholar 

  • Wasserman, R. H., and A. N. Taylor: Vitamin D-dependent calcium-binding protein—response to some physiological and nutritional variables. J. Biol. Chem.243, 3987 (1968).

    PubMed  Google Scholar 

  • Weber, C. W., and B. L. Reid: Effect of dietary cadmium on mice. Toxicol. Appl. Pharmacol.14, 420 (1969).

    Article  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Public Health, Sapporo Medical College, S.1, W. 17, 060, Sapporo, Japan

    Naoki Sugawara

Authors
  1. Naoki Sugawara
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Sugawara, N. Inhibitory effect of cadmium on calcium absorption from the rat duodenum. Arch. Environ. Contam. Toxicol. 5, 167–175 (1977). https://doi.org/10.1007/BF02220899

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02220899

Keywords

Search

Navigation