Abstract
Fixation and transfer of nitrogen (N) from alfalfa (Medicago sativa L.) to different grass species including timothy (Phleum pratense L.) and bromegrass (Bromus inermis Leyss) were studied under field conditions, using the15N dilution technique.
The percentage of alfalfa N derived from fixation (%NF) increased throughout the growing seasons and ranged from 62 to 83%. Nitrogen transfer (NT) from alfalfa to associated grasses was evident and contributed 26,46 and 38% of the total annual N yield of associated grasses or represented absolute amounts of 5, 20 and 19 kg N ha−1 during the first, second and third year, respectively. The gradual and consistent percentage of NT that occurred before first harvest indicated that this transfer is a result of a direct excretion of N compounds from alfalfa root systems. Decomposition of root and nodule debris seems to contribute to the NT from alfalfa to associated grasses in the later cuts. All grass species benefitted similarly from alfalfa, although earlier maturing species with greater competitive ability were slightly more responsive.
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References
Baylor J E 1974 Satisfying the nutritional requirements of grasslegume mixtures.In Forage Fertilization. Ed. D A Mays, America Society of Agronomy, Madison, Wisconsin.
Bell F and Nutman P S 1971 Experiments on nitrogen fixation by nodulated lucerne. Plant and Soil Special Vol, 231–261.
Broadbent F E, Nakashima T and Chang G Y 1982 Estimation of nitrogen fixation by isotope dilution in field and green-house experiments. Agron. J. 74, 625–628.
Brophy L S, Heichel G H and Russell M P 1987 Nitrogen transfer from forage legume to grass in a systematic planting design. Crop Sci. 27, 753–758.
Burton J C 1975 Nodulation and symbiotic nitrogen fixation.In Alfalfa Science and Technology. Ed. C H Hanson. pp 229–246. America Society of Agronomy, Madison, Wisconsin.
Butler G W and Bathurst N O 1956 The underground transference of nitrogen from clover to associated grass. Proceedings of the 7th International Grassland Congress, Palmerston North, N.Z., pp 168–175.
Chujo H and Daimon H 1984 Plant growth and fate of nitrogen in mixed cropping, intercropping and crop rotation. I. Growth acceleration of some temperate grasses in early stage of mixed cropping with red clover. Japan. J. Crop Sci. 53, 213–221.
Dart P J and Wildon D C 1970 Nodulation and nitrogen fixation byVigna sinensis andVicia atropurpurea: The influence of concentration, form and site of application of combined nitrogen. Aust. J. Agric. Res. 21, 45–56.
Dilz K and Mulder E G 1962 Effect of associated growth on yield and nitrogen content of legume and grass plants. Plant and Soil 16, 229–238.
Eaglesham A R J, Hassouna S and Seegers R 1983 Fertilizer N effects on N2 fixation by cowpea and soybean. Agron. J. 75, 61–66.
Erdman L W and Ura-Mae M 1953 Strain variation ofRhizobium meliloti on three varieties ofMedicago sativa. Agron. J. 45, 625–629.
Haystead A and Marriott C 1979 Transfer of legume nitrogen to associated grass. Soil Biol. Biochem. 11, 99–104.
Heichel G H, Barnes D K and Vance C P 1981 Nitrogen fixation of alfalfa in the seeding year. Crop Sci. 21, 330–335.
Henzell E F 1962 Nitrogen fixation and transfer by some tropical and temperate pasture legumes in sand culture. Aust. J. Exp. Agric. Anim. Husb. 2, 132–140.
Lawn R J and Brun W A 1974 Symbiotic nitrogen fixation in soybeans III. Effect of supplemental nitrogen and intervarietal grafting. Crop Sci. 14, 22–25.
Ross P J, Martin A E and Henzell E F 1964 A gas-tight growth chamber for investigating gaseous nitrogen changes in the soil∶plant∶atmosphere system. Nature 204, 444–447.
Simpson J R 1965 The transference of nitrogen from pasture legumes to an associated grass under several systems of managements in pot culture. Aust. J. Agric. Res. 16, 915–926.
Ta T C, Macdonald F D H and Faris M A 1986 Excretion of assimilated nitrogen from N2 fixed by nodulated roots of alfalfa (Medicago sativa L.). Can. J. Bot. 64, 2063–2067.
Ta T C and M A Faris 1987a Species variations in the fixation and transfer of nitrogen from legumes to associated grasses. Plant and Soil 98, 265–274.
Ta T C and Faris M A 1987b Effects of alfalfa proportion and clipping frequencies on timothy-alfalfa mixtures. II Nitrogen fixation and transfer. Agron. J. 79, 820–824.
West C P and Wedin W F 1985 Dinitrogen fixation in alfalfaorchardgrass pastures. Agron. J. 77, 89–94.
Whitney A S and Kanchiro Y 1967 Pathways of nitrogen transfer in some tropical legume-grass associations. Agron. J. 59, 585–588.
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Contribution No. 1159 from the Plant Research Centre
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Burity, H.A., Ta, T.C., Faris, M.A. et al. Estimation of nitrogen fixation and transfer from alfalfa to associated grasses in mixed swards under field conditions. Plant Soil 114, 249–255 (1989). https://doi.org/10.1007/BF02220805
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DOI: https://doi.org/10.1007/BF02220805