Abstract
This study was conducted to examine the effects of varying N rates and cropping systems (mixedversus pure stand) on the suitability of oats (Avena sativa L.) for estimating N2 fixed in sequentially harvested vetch (Vicia sativa L.) over two growing seasons (1984–85 and 1985–86). The N rates were, 20 and 100 kg N ha−1 in 1984–85 and 15 and 60 kg N ha−1 in 1985–86. In the 1984–85 season, vetch at maturity derived 76 and 63% N from fixation at the high and low N rates respectively. The corresponding values for the second season were 66 and 42%. Except in the 1985–86 season when some significantly higher values of % N2 fixed were estimated by using the reference crop grown at the higher (A-value approach) than at the lower N rate (isotope-dilution approach), both approaches resulted in similar measurements of N2 fixed. In the 1984–85 season, similar values of N2 fixed were obtained using either the pure or mixed stand oats reference crops. Although in the 1985–86 season, the mixed reference crop occasionally estimated lower % N2 fixed than pure oats, total N2 fixed estimates were always similar (P<0.05). Thus, in general, N fertilization and cropping system of the reference crop did not significantly influence estimates of N2 fixation.
Similar content being viewed by others
References
AOAC 1975 Official Methods of Analysis. 12th edition (ed. W Worwitz). Washington, D.C.: Association of Official Analytical Chemists.
Broadbent F E, Nakashima T and Chang Grace Y 1982 Estimation of nitrogen fixation by isotope dilution in field and greenhouse experiments. Agron. J. 74, 625–628.
Danso S K A 1986 Review: estimation of N2 fixation by isotope dilution: An appraisal of techniques involving15N enrichment and their application — Comments. Soil Biol. Biochem. 243–244.
Danso S K A, Zapata F, Hardarson G and Fried M 1987 Nitrogen fixation in fababeans as affected by plant population density in sole or intereropped systems with barley. Soil Biol. Biochem. 19, 411–415.
Fiedler R and Proksch G 1975 The determination of nitrogen-15 by emission and mass spectrometry in biological analysis: A review. Anal. Chim. Acta. 78, 1–62.
Fried M and Broeshart H 1975 An independent measurement of the amount of nitrogen fixed by legume crops. Plant and Soil 43, 707–711.
Fried M and Middelboe V 1977 Measurement of amount of nitrogen fixed by a legume crop. Plant and Soil 47, 713–715.
Fried M, Danso S K A and Zapata F 1983 The methodology of measurement of N2 fixation by non-legumes as inferred from field experiments with legumes. Can. J. Microbiol. 29, 1053–1062.
Hardarson G, Danso S K A and Zapata F 1987 Dinitrogen fixation measurements in alfalfa-ryegrass swards using nitrogen-15 and the influence of the reference crop. Crop Sci. 28, 101–105.
Hardarson G, Zapata F and Danso S K A 1984 Effect of plant genotype and nitrogen fertilizer on symbiotic nitrogen fixation by soybean cultivars. Plant and Soil 82, 397–405.
McAuliffe C, Chamblee D S, Uribe-Arago H and Woodhouse Jr W W 1958 Influence of inorganic nitrogen on nitrogen fixation by legumes as revealed by15N. Agron. J. 55, 334–337.
Rennie R J and Dubetz S 1986 Nitrogen-15-determined nitrogen fixation in field-grown chickpea, lentil, fababean, and field pea. Agron. J. 78, 654–660.
Ruschel A P, Vose P B, Victoria R L and Salati E 1979 Comparison of isotope techniques and non-nodulating isolines to study the effect of ammonium fertilization on dinitrogen fixation in soybean.Glycine max. Plant and Soil 53, 513–525.
Ta T C and Faris M A 1987 Species variation of nitrogen from legumes to associated grasses. Plant and Soil 98, 265–274.
Walker T W, Adams A F R and Orchiston H D 1956 Fate of labeled nitrate and ammonium nitrogen when applied to grass and clover grown separately and together. Soil Sci. 81, 339–351.
Witty J F 1983 Estimating N2-fixation in the field using15N-labeled fertilizer: Some problems and solutions. Soil. Biol. Biochem. 15, 631–639.
Zapata F, Danso S K A, Hardarson G and Fried M 1987 Nitrogen translocation in field-grown fababean. Agron. J. 79, 505–509.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Papastylianou, I., Danso, S.K.A. Effect of nitrogen fertilization and cropping system of the reference crop on estimation of N2 fixation by vetch using15N methodology. Plant Soil 114, 227–233 (1989). https://doi.org/10.1007/BF02220802
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF02220802