Preparation of extracts of culture liquids for gas-chromatographic determination of acidic fermentation products
- 28 Downloads
An extraction method coupled with gas-chromatographic analysis has been devised for the determination of small amounts of C1-C7 branched and straight-chain saturated fatty acids as well as 18 mono-, di- and tricarboxylic acids and related compounds, the latter being analyzed either as their methylesters or as their trimethylsilyl-derivatives. Methods for extracting non-volatile acids or both volatile and non-volatile acids are described for large and small volumes of culture media. The proposed methods have been successfully applied to the determination of non-acidic fermentation products, known amounts of alcohols, and to volatile and non-volatile acids in bacterial fermentation solutions, namely a batch culture ofLactobacillus casei subsp.rhamnosus ATCC 7469. A carbon balance sheet of this organism's metabolic behaviour throughout growth is proposed.
KeywordsAlcohol Extraction Method Small Volume Saturated Fatty Acid Culture Liquid
Unable to display preview. Download preview PDF.
- Bachur, N. R. 1965. A method for detecting organic acids on thin-layer and paper chromatograms. - Anal. Biochem.13: 463–468.Google Scholar
- Doelle, H. W. 1966. Preparation of extracts of culture liquids for gas-chromatographic determination of non-acidic fermentation products. - Antonie van Leeuwenhoek32: 373–380.Google Scholar
- Doelle, H. W. 1969. Gas Chromatographic separation and determination of micro quantities of C1-C7 branched and straight-chain saturated fatty acids. - J. Chromatog.39: 398–406.Google Scholar
- Doelle, H. W. andManderson, G. J. 1969. A rapid quantitative determination method for lactic acid as fermentation endproduct. - J. Gen. Microbiol.Google Scholar
- Huggett, A. St. G. andNixon, D. A. 1957. Enzymic determination of blood glucose. - Biochem. J.66: 12P.Google Scholar
- Harmon, M. A. andDoelle, H. W. 1969. Gas Chromatographic separation and determination for microquantities of the esters of the tricarboxylic acid cycle acids and related compounds. - J. Chromatog.42: 157–169.Google Scholar
- James, A. T. 1960. Qualitative and quantitative determination of the fatty acids by gas-liquid chromatography. IV. Preparation of stationary phases,In D. Glick, [ed.]. Methods Biochem. Anal. Vol. 8. - Interscience Publ., New York.Google Scholar
- Neish, A. C. 1952. Analytical methods for bacterial fermentations, 2nd revision. - Nat. Res. Council of Canada Report No. 46-8-3.Google Scholar
- Report by the Taxonomic Subcommittee on lactob: cilli and closely related organisms (1968). Type strains ofLactobacillus species. - Ameri in Type Culture Collection, Rockville, Maryland, U.S.A.Google Scholar
- Skerman, V. B. D. 1967. A guide to the identification of the genera of bacteria, 2nd ed. - Williams and Wilkins, Baltimore, p. 247.Google Scholar
- Ting, I. P. andDugger, W. D., Jr. 1965. Separation and detection of organic acids on silica gel. - Analyt. Biochem.12: 571–578.Google Scholar