Antonie van Leeuwenhoek

, Volume 35, Issue 1, pp 467–478 | Cite as

Preparation of extracts of culture liquids for gas-chromatographic determination of acidic fermentation products

  • H. W. Doelle
  • G. J. Manderson


An extraction method coupled with gas-chromatographic analysis has been devised for the determination of small amounts of C1-C7 branched and straight-chain saturated fatty acids as well as 18 mono-, di- and tricarboxylic acids and related compounds, the latter being analyzed either as their methylesters or as their trimethylsilyl-derivatives. Methods for extracting non-volatile acids or both volatile and non-volatile acids are described for large and small volumes of culture media. The proposed methods have been successfully applied to the determination of non-acidic fermentation products, known amounts of alcohols, and to volatile and non-volatile acids in bacterial fermentation solutions, namely a batch culture ofLactobacillus casei subsp.rhamnosus ATCC 7469. A carbon balance sheet of this organism's metabolic behaviour throughout growth is proposed.


Alcohol Extraction Method Small Volume Saturated Fatty Acid Culture Liquid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Bachur, N. R. 1965. A method for detecting organic acids on thin-layer and paper chromatograms. - Anal. Biochem.13: 463–468.Google Scholar
  2. Doelle, H. W. 1966. Preparation of extracts of culture liquids for gas-chromatographic determination of non-acidic fermentation products. - Antonie van Leeuwenhoek32: 373–380.Google Scholar
  3. Doelle, H. W. 1969. Gas Chromatographic separation and determination of micro quantities of C1-C7 branched and straight-chain saturated fatty acids. - J. Chromatog.39: 398–406.Google Scholar
  4. Doelle, H. W. andManderson, G. J. 1969. A rapid quantitative determination method for lactic acid as fermentation endproduct. - J. Gen. Microbiol.Google Scholar
  5. Huggett, A. St. G. andNixon, D. A. 1957. Enzymic determination of blood glucose. - Biochem. J.66: 12P.Google Scholar
  6. Harmon, M. A. andDoelle, H. W. 1969. Gas Chromatographic separation and determination for microquantities of the esters of the tricarboxylic acid cycle acids and related compounds. - J. Chromatog.42: 157–169.Google Scholar
  7. James, A. T. 1960. Qualitative and quantitative determination of the fatty acids by gas-liquid chromatography. IV. Preparation of stationary phases,In D. Glick, [ed.]. Methods Biochem. Anal. Vol. 8. - Interscience Publ., New York.Google Scholar
  8. Neish, A. C. 1952. Analytical methods for bacterial fermentations, 2nd revision. - Nat. Res. Council of Canada Report No. 46-8-3.Google Scholar
  9. Report by the Taxonomic Subcommittee on lactob: cilli and closely related organisms (1968). Type strains ofLactobacillus species. - Ameri in Type Culture Collection, Rockville, Maryland, U.S.A.Google Scholar
  10. Skerman, V. B. D. 1967. A guide to the identification of the genera of bacteria, 2nd ed. - Williams and Wilkins, Baltimore, p. 247.Google Scholar
  11. Ting, I. P. andDugger, W. D., Jr. 1965. Separation and detection of organic acids on silica gel. - Analyt. Biochem.12: 571–578.Google Scholar

Copyright information

© Swets & Zeitlinger 1969

Authors and Affiliations

  • H. W. Doelle
    • 1
  • G. J. Manderson
    • 1
  1. 1.Department of MicrobiologyUniversity of Queensland, Medical SchoolBrisbaneAustralia

Personalised recommendations