Antonie van Leeuwenhoek

, Volume 35, Issue 1, pp 467–478 | Cite as

Preparation of extracts of culture liquids for gas-chromatographic determination of acidic fermentation products

  • H. W. Doelle
  • G. J. Manderson
Article

Abstract

An extraction method coupled with gas-chromatographic analysis has been devised for the determination of small amounts of C1-C7 branched and straight-chain saturated fatty acids as well as 18 mono-, di- and tricarboxylic acids and related compounds, the latter being analyzed either as their methylesters or as their trimethylsilyl-derivatives. Methods for extracting non-volatile acids or both volatile and non-volatile acids are described for large and small volumes of culture media. The proposed methods have been successfully applied to the determination of non-acidic fermentation products, known amounts of alcohols, and to volatile and non-volatile acids in bacterial fermentation solutions, namely a batch culture ofLactobacillus casei subsp.rhamnosus ATCC 7469. A carbon balance sheet of this organism's metabolic behaviour throughout growth is proposed.

Keywords

Alcohol Extraction Method Small Volume Saturated Fatty Acid Culture Liquid 

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Copyright information

© Swets & Zeitlinger 1969

Authors and Affiliations

  • H. W. Doelle
    • 1
  • G. J. Manderson
    • 1
  1. 1.Department of MicrobiologyUniversity of Queensland, Medical SchoolBrisbaneAustralia

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