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Isolierung von Steringemischen aus Fetten und Ölen

II. Mitteilung Fällung mit Digitonin

Isolation of total sterols from fats and oils

II. Precipitation by digitonin

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Summary

Several sources of errors were encountered in frequently used procedures for the precipitation of sterols by digitonin. It was found that triterpenes and other products are also precipitated with this reagent. Quantitative isolation of the sterols requires a large excess of digitonin which cannot be completely removed from the precipitate.

Widely accepted procedures that yield “quantitative recoveries” were found to complex the sterols incompletely but to lead to precipitates that contain an excess of reagent which compen sates for this loss.

Zusammenfassung

Bei den gebräuchlichen Verfahren zur Fällung von Sterinen in Form der Digitonide wurden Fehlerquellen beobachtet. Dadurch werden sowohl die quantitative Bestimmung der Gesamtsterine nach dieser Methode unsicher als auch evtl. angeschlossene chromatographische Trennungen der Steringemische verfälscht. Arbeitsbedingungen, die „theoretische Ausbeuten” liefern, ergeben eine unvollständige Fällung, in welcher der Verlust durch einen Digitonin-Überschoß im Niederschlag „ausgeglichen” wird.

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Authors and Affiliations

  1. Bundesanstalt für Fettforschung, Piusallee 76, D-44, Münster, Bundesrepublik Deutschland

    E. Homberg &  A. Seher

Authors
  1. E. Homberg
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  2. A. Seher
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Homberg, E., Seher, A. Isolierung von Steringemischen aus Fetten und Ölen. Z Lebensm Unters Forch 149, 129–133 (1972). https://doi.org/10.1007/BF02215681

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  • DOI: https://doi.org/10.1007/BF02215681

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