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The enzymatic activities in the alkali-Burnt rabbit cornea

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Abstract

Alkali-burnt corneas of the rabbits with 1 N NaOH were studied periodically for enzymatic activities by biochemical methods. There was significant increase of aldolase (ALD) activity both in corneal epithelium and stroma 1, 2, 3 and 4 weeks after alkali burns. Glucose-6-phosphate dehydrogenase (G6PD) activity was significantly decreased in epithelium and was absent in stroma. Thus the breakdown of glucose would be present preferably in the Embden-Meyerhoff pathway instead of the pentose phosphate shunt. Lactate dehydrogenase (LDH) activity of corneal epithelium and stroma was significantly decreased 1, 2, 3, and 4 weeks after alkali burns and the possible pathway of glycolysis might channel to citric acid cycle, in which malate dehydrogenase (MDH) could indicate the important role in this pathway.

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Author notes

  1. V. Chayakul

    Present address: Department of Ophthalmology, Faculty of Medicine, Prince of Songkla University, Haadyai, Thailand

Authors and Affiliations

  1. Department of Ophthalmology, Faculty of Medicine, Rheinisch-Westfälische Technische Hochschule, Goethestr. 27/29, D-5100, Aachen, Federal Republic of Germany

    V. Chayakul & M. Reim

Authors
  1. V. Chayakul
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  2. M. Reim
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Additional information

Dr. Chayakul is the recipient of a research grant from the German Academic Exchange Service.

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Chayakul, V., Reim, M. The enzymatic activities in the alkali-Burnt rabbit cornea. Graefe's Arch Clin Exp Ophthalmol 218, 145–148 (1982). https://doi.org/10.1007/BF02215652

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  • DOI: https://doi.org/10.1007/BF02215652

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