Veterinary Research Communications

, Volume 9, Issue 1, pp 239–244 | Cite as

Isolation of pigeon herpes encephalomyelitis virus in Saudi Arabia

  • M. A. Shalaby
  • M. A. El-Sisi
  • O. E. Ismail
  • A. I. Afaleque
Article
  • 29 Downloads

Abstract

A virus was isolated from the brains of pigeons suffering from nervous disorders in different localities of the Eastern Province, Kingdom of Saudi Arabia. The new isolate caused a high morbidity, ranging from 33% to 50%, and a mortality rate which reached 40%.

The virus produced pinpoint greyish pock lesions on the chorioallantoic membrane of embryonated hens' eggs and induced syncytial formation followed by rounding and lysis of the cells in chicken embryo fibroblast cultures. Virus infectivity was significantly reduced following treatment by 20% ether or chloroform. The isolated virus was identified as pigeon herpes encephalomyelitis virus by serum-neutralization, agar gel diffusion and fluorescent antibody staining techniques.

Keywords

Encephalomyelitis Chicken Embryo Staining Technique Fibroblast Culture Fluorescent Antibody 

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Copyright information

© Elsevier Science Publishers B.V 1985

Authors and Affiliations

  • M. A. Shalaby
    • 1
  • M. A. El-Sisi
    • 1
  • O. E. Ismail
    • 1
  • A. I. Afaleque
    • 1
  1. 1.College of Veterinary Medicine and Animal ResourcesKing Faisal UniversityAl-Ahsa(Saudi Arabia)

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