Veterinary Research Communications

, Volume 8, Issue 1, pp 15–23 | Cite as

Growth of Chlamydia in pig lung alveolar macrophages; Preparation of macrophages and demonstration of growth

  • J. W. C. Hood
  • D. A. McMartin
  • J. W. Harris
Research Articles

Abstract

The harvesting, storing and utilisation in tissue culture of lung alveolar macrophages from 2–3 weeks old piglets were investigated. An average yield of 4×108 cells was obtained, which in culture usually remained healthy for at least 8 days. In liquid nitrogen at −173°C about 50% of the cells were still viable after 3 months. Pig cells sustained the growth of ovineChlamydiapsittaci and a rising titre was demonstrated over 4 passages.

Keywords

Public Health Nitrogen Liquid Nitrogen Tissue Culture Veterinary Medicine 

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Copyright information

© Elsevier Science Publishers B.V. 1984

Authors and Affiliations

  • J. W. C. Hood
    • 1
  • D. A. McMartin
    • 1
  • J. W. Harris
    • 2
  1. 1.Veterinary LaboratoryMinistry of Agriculture, Fisheries and FoodLasswadeScotland
  2. 2.Ministry of Agriculture, Fisheries and FoodBeverleyEngland

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