Effects of soil applied herbicides on leaf nitrate reductase and crude protein in the leaf and seed of two cowpea cultivars
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A glass-house study was conducted to determine the effects of four commonly used herbicides (pendimethalin, metobromuron, metolachlor and prometryne) applied pre-emergence at rates of 0, 0.125, 0.625 and 1.25 kg ha−1, on leaf nitrate concentration (NO3−C), nitrate reductase activity (NRA), leaf crude protein and seed protein in two cowpea cultivars, 60 day (60D) and Ife brown (IB).
Control and treated plants of both cultivars showed separate peaks for NO3−C and NRA, 49 days after planting (DAP) and 35 DAP for 60D and IB respectively. Herbicide treatment generally enhanced NO3−C but tended to decrease NRA in both cultivars. Howver, metobromuron at 0.625 kg ha−1 increased NRA throughout the growth period with an optimum increase of 52.5%, over the control, at 35 DAP. Pendimethalin increased NO3−C NRA and leaf protein but did not influence seed protein appreciably. In contrast metobromuron increased NO3−C, decreased NRA, but increased seed protein by 29.6% over the control at 0.125 kg ha−1 in 60D. Metolachlor and prometryne were most inhibitory to seed protein development. In addition, metolachlor reversed the interdependence of NO3−C and NRA.
Key wordscowpea crude protein herbicides nitrate concentration nitrate reductase activity
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- Ajakaiye C O 1981 Influence of soil application of nitrogen on nitrate reductase activity, leaf and grain protein content in sorghum. Plant and Soil 60, 423–434.Google Scholar
- Eilrich C L and Hageman R H 1973 Nitrate reductase activity and its relationship to accumulation of vegetative and grain nitrogen in wheat (Triticum aestivum). Crop Sci. 13, 59–60.Google Scholar
- Esmama B V and Juliano B O 1976 The effect of subherbicidal application of simetryne and benzomarc on the N-metabolism of rice seedlings. Phillip. J. Biol. 5, 315–324.Google Scholar
- Gallagher L G, Khairy M S, Rains D W, Qualset C O and Huffaker R G 1983 Nitrogen assimilation in common wheat differing in potential nitrate reductase activity and tissue nitrate concentration. Crop Sci. 23, 913–919.Google Scholar
- Huffaker R G and Peterson J 1974 Protein turn-over in plants and possible means of its regulation. Annu. Rev. Plant Physiol. 18, 301–324.Google Scholar
- Johnson C B, Wittington W J and Blackwood G C 1976 Nitrate reductase as a predictive test for crop yield. Nature 262, 133–134.Google Scholar
- Kees H and Kramrey G 1978 Effects of grassland herbicides on the nitrate content of green forage. Gesunde Pflanzen 30 (10), 245–250.In Weed Abstr. (1979).Google Scholar
- Kleeper L A 1971 Generation of reduced nicotinamide adenine dinuoleotide for NO3-reduction in plants. Plant Physiol. 48, 580–590.Google Scholar
- Pillai C G, Davis D E and Truelove B 1979 Effects of metolachlor on germination, growth, leucine uptake and protein synthesis. Weed Sci. 27, 634–637.Google Scholar
- Radin J W 1973In vivo assay of nitrate reductase in cotton leaf discs. Plant Physiol. 51, 332–336.Google Scholar
- Schlesier G 1977 Nitratreduktase aktiver in Blättern und Früchten von verschiedener Leguminosen. Biochem. Physiol. Plfanzen 171, 511–23.Google Scholar
- Thiobodeaux P S and Jaworski E G 1975 Patterns of N-utilization in the soybean. Planta 1277, 133–147.Google Scholar
- Warmund M R, Kerr H D and Paterns E J 1985 Lipid metabolism in grain sorghum (Sorghum bicolour) treated with Alachlor plus flurazole. Weed Sci. 33, 25–28.Google Scholar
- Williams P C 1964 The colorimetric determination of total nitrogen in feeding stuffs. Analyst 89, 276–281.Google Scholar
- Wu M T, Singh B and Salunkhe D K 1972 Influence of foliar application of S-triazine compounds on fresh weight, dry weight, chemical composition and enzymatic activity of pea and sweet corn seedlings. J. Exp. Bot. 83, 793–800.Google Scholar