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Journal of Assisted Reproduction and Genetics

, Volume 11, Issue 7, pp 367–372 | Cite as

Coculture of mouse embryos with cryopreserved human oviduct epithelial cells

  • Kazuhiko Hoshi
  • Yoshie Kanno
  • Haruo Katayose
  • Kaoru Yanagida
  • Rumi Suzuki
  • Akira Sato
Animal Investigations

Abstract

Purpose

The effect of coculturing mouse embryos with cryopreserved human oviduct epithelial cells was investigated. The cryopreserved cells in Cellbanker were thawed and cultured in Richard D. Goldsby culture medium to establish monolayers. Two-cell-stage mouse embryos were cultured alone (control group) or cocultured with monolayers established from cryopreserved cells (cryopreserved coculture group) or from fresh cells (fresh coculture group). The rates of embryo development and the qualities of the blastocysts in the three groups were compared.

Results

The two coculture groups had significantly higher blastocyst development rates (cryopreserved coculture group, 81.6%; fresh coculture group, 82.2%) than the control group (63.1%). The two coculture groups had significantly more blastomeres (cryopreserved coculture group, 108.3 ± 25.9; fresh coculture group, 108.4 ± 25.1) than the control group (87.7 ± 31.9).

Conclusion

The method of cryopreservation of human oviduct epithelial cells using Cellbanker is simpler than conventional cryopreservation methods. These cryopreserved human oviduct epithelial cells may provide a constant supply of cells for coculture for in vitro fertilization and embryo transfer.

Key words

coculture cryopreservation human oviduct epithelial cell blastocyst mouse embryo 

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Copyright information

© Plenum Publishing Corporation 1994

Authors and Affiliations

  • Kazuhiko Hoshi
    • 1
  • Yoshie Kanno
    • 1
  • Haruo Katayose
    • 1
  • Kaoru Yanagida
    • 1
  • Rumi Suzuki
    • 1
  • Akira Sato
    • 1
  1. 1.Department of Obstetrics and GynecologyFukushima Medical CollegeFukushimaJapan

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