Journal of Assisted Reproduction and Genetics

, Volume 11, Issue 7, pp 367–372 | Cite as

Coculture of mouse embryos with cryopreserved human oviduct epithelial cells

  • Kazuhiko Hoshi
  • Yoshie Kanno
  • Haruo Katayose
  • Kaoru Yanagida
  • Rumi Suzuki
  • Akira Sato
Animal Investigations



The effect of coculturing mouse embryos with cryopreserved human oviduct epithelial cells was investigated. The cryopreserved cells in Cellbanker were thawed and cultured in Richard D. Goldsby culture medium to establish monolayers. Two-cell-stage mouse embryos were cultured alone (control group) or cocultured with monolayers established from cryopreserved cells (cryopreserved coculture group) or from fresh cells (fresh coculture group). The rates of embryo development and the qualities of the blastocysts in the three groups were compared.


The two coculture groups had significantly higher blastocyst development rates (cryopreserved coculture group, 81.6%; fresh coculture group, 82.2%) than the control group (63.1%). The two coculture groups had significantly more blastomeres (cryopreserved coculture group, 108.3 ± 25.9; fresh coculture group, 108.4 ± 25.1) than the control group (87.7 ± 31.9).


The method of cryopreservation of human oviduct epithelial cells using Cellbanker is simpler than conventional cryopreservation methods. These cryopreserved human oviduct epithelial cells may provide a constant supply of cells for coculture for in vitro fertilization and embryo transfer.

Key words

coculture cryopreservation human oviduct epithelial cell blastocyst mouse embryo 


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  1. 1.
    Gondolfi F, Moor RM: Stimulation of early embryonic development in the sheep by co-culture with oviduct epithelial cells. J Reprod Fertil 1987;81:23–28Google Scholar
  2. 2.
    Eystone WH, Vignieri J, First NL: Co-culture of early bovine embryos with oviductal epithelium. Theriogenology 1987;27:228Google Scholar
  3. 3.
    Ouhibi N, Menezo Y, Benet G, Nicollet B: Culture of epithelial cells derived from the oviduct of different species. Hum Reprod 1989;4:229–235Google Scholar
  4. 4.
    Bongso A, Ng SC, Sathananthan H, Ng PL, Rauff M, Ratnam SS: Establishment of human ampullary cell cultures. Hum Reprod 1989;4:486–494Google Scholar
  5. 5.
    Henriksen T, Tanbo T, Abyholm T, Oppedal BR, Claussen OP, Hovig T: Epithelial cells from fallopian tube in culture. Hum Reprod 1990;5:25–31Google Scholar
  6. 6.
    Takeuchi K, Nagata Y, Sandow BA, Hodgen GD: Primary culture of human fallopian tube epithelial cells and coculture of early mouse pre-embryos. Mol Reprod Dev 1992;32:236–242Google Scholar
  7. 7.
    Freeman MR, Bastias MC, Hill GA, Osteen KG: Coculture of mouse embryos with cells isolated from the human ovarian follicle, oviduct, and uterine endometrium. Fertil Steril 1993;59:138–142Google Scholar
  8. 8.
    Bongso A, Ng SC, Sathananthan H, Ng PL, Rauff M, Ratnam S: Improved quality of human embryos when cocultured with human ampullaly cells. Hum Reprod 1989;4:706–713Google Scholar
  9. 9.
    Ouhibi N, Hamidi J, Guillaud J, Menezo Y: Co-culture of 1-cell mouse embryos on different cell supports. Hum Reprod 1990;5:737–743Google Scholar
  10. 10.
    Sakkas P, Trouson AO: Co-culture of mouse embryos with oviduct and uterine cells prepared from mice at different days of pregnancy. J Reprod Fertil 1990;90:109–118Google Scholar
  11. 11.
    Carney EW, Tobback C, Ellington JE, Foote RH: Co-culture of rabbit 2-cell embryos with rabbit oviduct epithelial cell and other somatic cells. Mol Reprod Dev 1990;27:209–215Google Scholar
  12. 12.
    Eyestone WH, First NL: Co-culture of early cattle embryos to the blastocyst stage with oviductal tissue or in conditioned medium. J Reprod Fertil 1989;85:715–720Google Scholar
  13. 13.
    Pollard JW, Xu KP, Rorie R, King WA, Betteridge KJ: Influence of various oviductal epithelial cell culture systems on the development of early cleavage stage bovine embryos in vitro. Theriogenology 1989;31:239Google Scholar
  14. 14.
    Ellington JE, Carney EW, Farrell PB, Simkin ME, Foote RH: Bovine 1–2 cell embryo development using a simle medium in three oviduct epithelial cell coculture systems. Biol Reprod 1990;43:97–104Google Scholar
  15. 15.
    Aoyagi Y, Fukui Y, Iwazumi Y, Urakawa M, Minegishi Y, Ono H: Effects of culture systems on development of invitro fertilized bovine ova into blastocysts. Theriogenology 1989;31:168Google Scholar
  16. 16.
    Menezo Y, Guerin JF, Czyba JC: Improvement of human early embryo development in vitro by coculture on monolayers of vero cells. Biol Reprod 1990;42:301–306Google Scholar
  17. 17.
    Menezo Y, Hazout A, Dumont M, Herbaut N, Nicollet B: Coculture of embryo on Vero cells and transfer of blastocysts in humans. Hum Reprod 1992;7(Suppl 1):101–106Google Scholar
  18. 18.
    Weimer K, Amborski G, Denniston R, Godke R: Use of a hormone-treated fetal uterine fibroblast monolayer for in vitro development for bovine embryos. Theriogenology 1987;27:294–299Google Scholar
  19. 19.
    Wiemer KE, Cohen J, Amborski GF, Wright G, Wiker S, Munyakazi L, Godke RA: In-vitro development and implantation of human embryos following culture on fetal bovine uterin fibroblast cells. Hum Reprod 1989;4:595–600Google Scholar
  20. 20.
    Menezo Y, Nicollet B, Herbaut N, Andre D: Freezing cocultured human blastocyst. Fertil Steril 1992;58:977–980Google Scholar
  21. 21.
    Wiemer KE, Hoffman DI, Maxson WS, Eager S, Muhlberger B, Fiore I, Cuervo M: Embryonic morphology and rate of implantation of human embryos following co-culture on bovine oviductal epithelial cells. Hum Reprod 1993;8:97–101Google Scholar
  22. 22.
    Bongso A, Ng SC, Fong CY, Ratnam S: Cocultures: A new lead in embryo quality improvement for assisted reproduction. Fertil Steril 1991;56:179–191Google Scholar
  23. 23.
    Bongso A, Ng SC, Fong CY, Ratnam S: Improved fertilization rates of human oocytes in co-culture. J Vitro Fertil Embryo Transfer 1991;8:216–221Google Scholar
  24. 24.
    Bongso A, Ng SC, Fong CY, Anandakumar C, Marshall B, Edirisinghe R, Ratnam S: Improved pregnancy rate after transfer of embryo grown in human fallopian tubal cell coculture. Fertil Steril 1992;58:569–574Google Scholar
  25. 25.
    Butzow R: The human fallopian tube contains placental protein 5. Hum Reprod 1989;4:17–20Google Scholar

Copyright information

© Plenum Publishing Corporation 1994

Authors and Affiliations

  • Kazuhiko Hoshi
    • 1
  • Yoshie Kanno
    • 1
  • Haruo Katayose
    • 1
  • Kaoru Yanagida
    • 1
  • Rumi Suzuki
    • 1
  • Akira Sato
    • 1
  1. 1.Department of Obstetrics and GynecologyFukushima Medical CollegeFukushimaJapan

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