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Veterinary Research Communications

, Volume 10, Issue 1, pp 317–324 | Cite as

Sulfoxidation of albendazole by a cytochrome P450-independent monooxygenase from rat liver microsomes

  • X. Fargetton
  • P. Galtier
  • P. Delatour
Article

Abstract

The in vitro biological oxidation of albendazole to albendazole sulfoxide by rat liver microsomes has been studied. This reaction corresponds to a NADPH-dependent enzymatic system, characterised by Km and Vm values of 53.6 μM and 0.59 nmole/mg protein per min.

The rate of sulfoxidation by liver microsomes of rats treated with phenobarbital, B-naphthoflavone, Aroclor 1254 and 3-methylcholanthrene was not increased. SKF 525A and metyrapone did not inhibit albendazole sulfoxidase.

Thiobenzamide and tranylcypromine decreased sulfoxidation to 48 and 52% of control values. The inhibition by tranylcypromine was competitive. Purified flavin adenine dinucleotide (FAD)-containing monooxygenase from hog liver microsomes catalysed sulfoxidation of albendazole (V=0.52 nmole/nmole enzyme per min).

The present data demonstrate that sulfoxidation of albendazole in the rat liver is not catalysed by a cytochrome P450-dependent monooxygenase and suggest that albendazole is a substrate for FAD-containing monooxygenase (FMO).

Keywords

Sulfoxide Phenobarbital Liver Microsome Flavin Albendazole 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Elsevier Science Publishers B.V 1986

Authors and Affiliations

  • X. Fargetton
    • 1
  • P. Galtier
    • 2
  • P. Delatour
    • 1
  1. 1.Laboratoire de Biochimie INRA-54.189Ecole Nationale Vétérinaire de LyonCharbonnières CédexFrance
  2. 2.Laboratoire de Pharmacologie-Toxicologie INRAToulouseFrance

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