Veterinary Research Communications

, Volume 10, Issue 1, pp 213–221 | Cite as

The occurrence of paranuclear vacuoles in sheep ruminal epithelium treated with distilled water in vitro and from sheep injected with a large quantity of water into the rumen

  • S. Ohwada
  • N. Ouchi
  • H. Tamate


In the histological preparation taken after animal death, paranuclear vacuoles (PV) in sheep ruminal epithelium were already present in high percentage (7.5–20.3%). With the incubation of the ruminal mucosae in distilled water (10–15°C) up to 120 min, PV occurrence did not alter. With incubation in NaCl solution, however, PV value decreased with time proportional to NaCl concentration (0.5, 0.85 or 1.5%). In the preparation taken by biopsy, on the other hand, PV were rare in ruminal epithelium (<0.3%). With the injection of warm water (10–141/ animal), however, PV occurrence in the ruminal epithelium was not affected. PV as cytoplasmic processes of the ruminal Langerhans cells are probably formed by the reaction of these cells to environmental changes in the tissue caused by animal death.


Public Health Environmental Change Veterinary Medicine Warm Water Cytoplasmic Process 
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  1. Alam, M.R. Poppi, D.P. and Sykes, A.R., 1983. Intake, digestibility and retention time of 2 forages by kids and lambs. Proc. N.Z. Soc. Anim. Prod., 43: 119–121.Google Scholar
  2. Dobson, A., 1959. Active transport through the epithelium of the reticulo-rumen sac. J. Physiol., 146: 235–251.Google Scholar
  3. Gemmell, R.T., 1973. Langerhans cells in the ruminal epithelium of the sheep. J. Ultrastruct. Res., 43: 256–259.Google Scholar
  4. Gihad, E.A., 1976. Intake, digestibility and nitrogen utilization of tropical natural grass hay by goats and sheep. J. Anim. Sci., 43: 879–883.Google Scholar
  5. Henrickson, K.B. and Habel, R.E., 1961. The morphology and sulfhydryl and disulfide reactions of the epithelium of the bovine forestomach during postnatal development. Anat. Rec., 139: 449–507.Google Scholar
  6. Hofmann, R.R., 1973. The Ruminant Stomach (Stomach structure and feeding habitats of east african game ruminant) East African Literature Bureau, Nairobi, pp. 1–345.Google Scholar
  7. Lavker, R. Chalupa, W. and Duckey, J.F., 1969. An electron microscopic investigation of rumen mucosa. J. Ultrastruct. Res., 28: 1–15.Google Scholar
  8. Nagatani, T., Kikuchi, T., Sakata, T. and Tamate, H., 1974. Identification of the so-called paranuclear vacuoles as the processes of the rumen Langerhans cells in the sheep. Tohoku J. Agr. Res., 25: 14–21.Google Scholar
  9. Ohwada, S. and Tamate, H., 1983a. Circadian rhythm of the mitotic index in the rumen epithelium of sheep. Tohoku J. Agr. Res., 33: 138–141.Google Scholar
  10. Ohwada, S. and Tamate, H., 1983b. Effect of adrenalin and noradrenalin on mitotic index in sheep rumen epithelium. Tohoku J. Agr. Res., 33: 142–145.Google Scholar
  11. Ohwada, S., Siomura, Y. and Tamate, H., 1984. A stathmokinetic study on the ruminal epithelium of sheep. Vet. Res. Commun., 8: 203–209.Google Scholar
  12. Sakata, T. and Tamate, H., 1974. Effect of the intermittent feeding in the mitotic index and the ultrastructure of basal cells of the ruminal epithelium in the sheep. Tohoku J. Agr. Res., 25: 156–163.Google Scholar
  13. Sakata, T. and Tamate, H., 1978. Influence of butyrate on the microscopic structure of ruminal mucosa in adult sheep. Jpn. J. Zootech. Sci., 49: 687–696.Google Scholar
  14. Shkolnik, A., Silanikove, N. and Brosh, A., 1983. Adaptation of desert ruminants to low quality or restricted diets and intermittent drinking. Proc. Int. Union Physiol. Sci., 15: 331.Google Scholar
  15. Steven, D.H. and Mearshall, A.B., 1970. Organization of the rumen epithelium. In: A.T. Phillipson (Editor), Physiology of Digestion and Metabolism in the Ruminant. Oriel Press, Newcastle upon Tyne, pp. 80–100.Google Scholar
  16. Tamate, H. and Fell, B. F., 1977. Cell deletion as a factor in the regulation of rumen epithelial populations. Vet. Sci. Commun., 1: 359–364.Google Scholar
  17. Tamate, H. Ishida, K. and Itikawa, O., 1964. The occurrence of of paranuclear vacuoles in the epithelial cells of rumen mucosa after treatment with distilled water in vitro. Tohoku J. Agr. Res., 14: 195–207.Google Scholar
  18. Wardrop, I.D., 1961a. Some preliminary observations on the histological development of the fore-stomachs of the lamb. I. Histological changes due to age in the period from 46 days of foetal life to 77 days of post-natal life. J. Agr. Sci., 57: 335–342.Google Scholar
  19. Wardrop, I.D., 1961b. Some preliminary observations on the histological changes due to the fore-stomachs of the lamb. II. The effects of diets on the histological development of the forestomachs of the lamb during post-natal life. J. Agr. Sci., 57: 343–346.Google Scholar

Copyright information

© Elsevier Science Publishers B.V 1986

Authors and Affiliations

  • S. Ohwada
    • 1
  • N. Ouchi
    • 1
  • H. Tamate
    • 1
  1. 1.Laboratory of Animal Morphology, Department of Animal Science, Faculty of AgricultureTohoku UniversitySendai(Japan)

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