Abstract
Virus synthesis in BALB/C mouse lung and kidney primary cultures infected with infectious bovine rhinotracheitis (IBR) virus started between 6 and 8 hours after virus inoculation and reached a maximum titer of 5.5 log10 plaque forming units (PFU) at 48 hours postinfection (PI). Cytopathic effect (CPE) in cell cultures occurred at 8–10 hours and over 90% of the cells had CPE by 48 to 72 hours PI. The bulk of the newly replicated virus (60–80%) was cell-associated as determined by plaque assay of extracellular and intracellular virus. Pulse-chase experiments demonstrated incorporation of radioactive precursors into viral DNA and protein macromolecules. Viral DNA synthesis was initiated between 2 and 4 hours PI and was maximum at 4 to 6 hours. Viral proteins were detected at 4 hours and peaked between 6 and 8 hours PI. Enzyme-linked immunosorbant assay (ELISA) confirmed synthesis of specific viral proteins, which gradually increased during the virus growth cycle. Abstract in French is given at the end of the article.
Resume
La synthèse du virus de la rhinotrachéite infectieuse bovine (virus IBR) sur des cultures primaires de poumon et de reins de souris (souche Balb/C) commençait entre 6 et 8 heures après inoculation du virus et atteignait un titre maximal de 5.5 log PFU (Plaque Forming Unit) 48 heures après infection. L'effet cytopathogenic (CPE) sur ces cultures cellulaires apparaissaient en 8 à 10 heures et plus de 90% des cellules montraient un CPE en 48 à 72 heures après infection. La détermination par la méthode des plages sous agarose, du virus extracellulaire et du virus intracellulaire, indiquait que 60–80% du virus synthétisé était associé avec les cellules. Les expériences de “pulsechase” demontraient l'incorporation des précurseurs radioactifs dans les protèines et ADN viraux. La synthèse virale d'ADN était initiée entre 2 et 4 heures après infection et était maximale en 4 à 6 heures. Les protéines virales étaient détectées en 4 heures avec un maximum de synthèse entre 6 et 8 heures après infection. Enzyme-linked immunosorbant assay (ELISA) confirmait la synthèse de protéines virales spécifiques qui montrait une augmentation graduelle pendant le cycle de replication du virus.
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Supported in part by grants 0831 from the Cooperative State Research Service of the US Department of Agriculture and published as contribution 85-160-J, Kansas Agricultural Experiment Station.
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Ghram, A., Minocha, H. Propagation of infectious bovine rhinotracheitis (bovine herpes-1) virus in murine primary cell cultures. Vet Res Commun 10, 45–55 (1986). https://doi.org/10.1007/BF02213964
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DOI: https://doi.org/10.1007/BF02213964