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Sperm stimulants can improve fertilization rates in male-factor cases undergoing IVF to the same extent as micromanipulation by partial zona dissection (PZD) or subzonal sperm insemination (SUZI): A randomized controlled study

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Abstract

Purpose

Our purpose was to evaluate the efficacy of direct insemination (IVF), micromanipulation by partial zona dissection (PZD), and subzonal sperm insemination (SUZI) using spermtreated with pentoxifylline (PF) ± 2-deoxyadenosine (2DA).

Results

The overall fertilization rate achieved was similar for all three fertilization techniques (33.1, 30.2, and 26.9% for IVF, SUZI, and PZD, respectively). Patients who had reduced fertilization in previous IVF attempts showed improved fertilization with sperm stimulants, either PF alone or PF in combination with 2DA in standard IVF. In certain cases, SUZI or PZD gave significantly improved fertilization rates in comparison to IVF.

Conclusion

Selective use of sperm stimulants in IVF can achieve fertilization for the majority of male-factor cases. However, PZD and SUZI techniques are useful, especially when sperm stimulants fail to achieve fertilization or achieve poor fertilization in direct insemination.

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References

  1. Yovich JM, Edirisinghe WR, Cummins JM, Yovich JL: Preliminary results using pentoxifylline in a pronuclear stage tubal transfer (PROST) program for severe male factor infertility. Fertil Steril 1988;50(1):179–181

    PubMed  Google Scholar 

  2. Yovich JM, Edirisinghe WR, Cummins JM, Yovich JL: Influence of pentoxifylline in severe male factor infertility. Fertil Steril 1990;53(4)715–722

    PubMed  Google Scholar 

  3. Yovich JM, Edirisinghe WR, Yovich JL: Use of the acrosome reaction to ionophore challenge in managing patients in an assisted reproduction program: a prospective, double-blind, randomized controlled study. Fertil Steril 1994;61(5):902–910

    PubMed  Google Scholar 

  4. Mahadevan MM, Trounson AO: The influence of seminal characteristics on the success rate of human in vitro fertilisation. Fertil Steril 1984;42(3):400–405

    PubMed  Google Scholar 

  5. Tesarik J, Thebault A, Testart J: Effect of pentoxifylline on sperm movement characteristics in normozoospermic and asthenozoospermic specimens. Hum Reprod 1992;7(9):1257–1263

    PubMed  Google Scholar 

  6. Yovich JL: Pentoxifylline: Actions and applications in assisted reproduction. Hum Reprod 1993;8(11):1786–1791

    PubMed  Google Scholar 

  7. Palermo G, Joris H, Devroey P, Van Steirteghem AC: Pregnancies after intracytoplasmic injection of single spermatozoon into an oocyte. Lancet 1992;340(8810):17–19

    PubMed  Google Scholar 

  8. Van Steirteghem AC, Jiaen L, Joris H,et al.: Higher success rate by intracytoplasmic sperm injection than by subzonal insemination—Report of a second series of 300 consecutive treatment cycles. Hum Reprod 1993;8(7):1055–1060

    PubMed  Google Scholar 

  9. Cummins JM, Jequier AM: Treating male infertility needs more clinical andrology, not less. Hum Reprod 1994;9(7):1214–1219

    PubMed  Google Scholar 

  10. World Health Organization: WHO Laboratory Manual for the Examination of Human Semen and Sperm-Cervical Mucus Interaction, 3rd ed. Cambridge: Cambridge University Press, 1992, p 107

    Google Scholar 

  11. Cummins JM, Pember SM, Jequier AM, Yovich JL, Hartman PE: A test of the human sperm acrosome reaction following ionophore challenge (ARIC): Relationship to fertility and other seminal parameters. J Androl 1991;12(2):98–103

    PubMed  Google Scholar 

  12. Cohen J, Malter H, Wright G, Kort H, Massey J, Mitchell D: Partial zona dissection of human oocytes when failure of zona pellucida penetration is anticipated. Hum Reprod 1989;4(4):435–442

    PubMed  Google Scholar 

  13. Yovich JL: Assisted reproduction for male factor infertility.In A Textbook of In Vitro Fertilization and Assisted Reproduction. PR Brinsden, PA Rainsbury (eds). Carnforth, Lancashire, UK: Parthenon, 1992; pp 311–323

    Google Scholar 

  14. Parinaud J, Vieitez G, Labal B, Richoilley G: Effect of sperm pretreatments on the results of sub-zonal insemination (SUZI). Hum Reprod 1994;9(1):110–112

    PubMed  Google Scholar 

  15. Lassalle B, Testart J: Human sperm injection into the perivitelline space (SI-PVS) of hamster oocytes: Effect of sperm pretreatment by calcium-ionophore A23187 and freezingthawing on the penetration rate and polyspermy. Gamete Res 1988;20:301–311

    PubMed  Google Scholar 

  16. Palermo G, Joris H, Devroey P, Van Steirteghem AC: Induction of acrosome reaction in human spermatozoa used for subzonal insemination. Hum Reprod 1992;7(2):248–254

    PubMed  Google Scholar 

  17. Tournaye H, Wieme P, Janssens R, Verheyen G, Devroey P, Vansteirteghem A: Incubation of spermatozoa from asthenozoospermic semen samples with pentoxifylline and 2-deoxyadenosine: Variability in hyperactivation and acrosome reaction rates. Hum Reprod 1994;9(11):2038–2043

    PubMed  Google Scholar 

  18. Junk SM, Edirisinghe WR, Yovich JL: Correlation of semen parameters to fertilisation rates in IVF and micromanipulation using pentoxifylline.In the Proceedings of the IX World Congress on In Vitro Fertilization and Alternate Assisted Reproduction, Aburumichet al. (eds). Volume 1, Monduzzi Editore, International Proceedings Division, Bologna, 1995, pp 557–560

    Google Scholar 

  19. Calderon G, Veiga A, Penella J, Barri PN: Two years of assisted fertilisation by partial zona dissection in male factor infertility patients. Fertil Steril 1993;60(1):105–109

    PubMed  Google Scholar 

  20. Garrisi GJ, Chin AJ, Dolan PM,et al: Analysis of factors contributing to success in a program of micromanipulationassisted fertilization. Fertil Steril 1993;59(2):366–374

    PubMed  Google Scholar 

  21. Cohen J, Alikani M, Adler A,et al.: Microsurgical fertilization procedures—The absence of stringent criteria for patient selection. J Assist Reprod Genet 1992;9(3):197–206

    PubMed  Google Scholar 

  22. Imoedemhe DAG, Sigue AB: Subzonal multiple sperm injection in the treatment of previous failed human in vitro fertilization. Fertil Steril 1993;59(1):172–176

    PubMed  Google Scholar 

  23. Cohen J, Alikani M, Malter HE: Partial zona dissection or subzonal sperm insertion: Microsurgical fertilisation alternatives based on evaluation of sperm and embryo morphology. Fertil Steril 1991;56(4):696–706

    PubMed  Google Scholar 

  24. Bonduelle M, Legein J, Buysse A, Devroey P, Van Steirteghem A, Liebaers I: Comparative follow-up study of 130 children born after ICSI and 130 children born after IVF. Abstracts of the 10th annual meeting of the ESHRE, Brussels, 1994, p 38

  25. Ord T, Patrizio P, Balmaceda JP, Asch RH: Can severe male factor infertility be treated without micromanipulation. Fertil Steril 1993;60(1):110–115

    PubMed  Google Scholar 

  26. Svalander P, Wikland M, Jakobsson AH, Forsberg AS: Subzonal insemination (SUZI) or in vitro fertilisation (IVF) in microdroplets for the treatment of male-factor infertility. J Assist Reprod Genet 1994; 11(3):149–155

    PubMed  Google Scholar 

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Authors and Affiliations

  1. PIVET Medical Centre, 166-168 Cambridge Street, 6007, Leederville, Perth, Western Australia

    W. Rohini Edirisinghe, Stephen Junk, Jeanne M. Yovich, Barbara Bootsma & John L. Yovich

Authors
  1. W. Rohini Edirisinghe
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  2. Stephen Junk
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  3. Jeanne M. Yovich
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  4. Barbara Bootsma
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  5. John L. Yovich
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Edirisinghe, W.R., Junk, S., Yovich, J.M. et al. Sperm stimulants can improve fertilization rates in male-factor cases undergoing IVF to the same extent as micromanipulation by partial zona dissection (PZD) or subzonal sperm insemination (SUZI): A randomized controlled study. J Assist Reprod Genet 12, 312–318 (1995). https://doi.org/10.1007/BF02213710

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  • DOI: https://doi.org/10.1007/BF02213710

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